To Plate or to Simply Unfreeze, That Is the Question for Optimal Plasmid Extraction

| Gabriel GonzalezGabriel Gonzalez | 0 Comment
heraeus-targets

Many molecular biology purposes require quick plasmid DNA extraction, spurring a number of research on easy methods to pace up the method. It’s frequently instructed in normal laboratory protocols to plate out frozen glycerol bacterial shares previous to micro organism incubation in liquid media and subsequent plasmid extraction, though the rationale for that is usually unexplained (apart from for the isolation of single colonies). Given the commonality and significance of this laboratory operation, such a apply is time-consuming and laborious.

To check the impression of this apply and the choice direct culturing technique, we investigated the affiliation between bacterial cell mass and its potential affect on plasmid yields from the two strategies. Our outcomes confirmed no distinction with preplating for 7 out of eight plasmid constructs used within the research, suggesting that direct glycerol restoration would not result in poorer plasmid yields. The findings assist the rationale for direct glycerol restoration for plasmid extraction, with out the necessity of an intermediate preplating step.

 

A plasmid toolbox for managed gene expression throughout the Proteobacteria

 

Managed gene expression is prime for the research of gene operate and our capability to engineer micro organism. Nonetheless, there’s at the moment no easy-to-use genetics toolbox that permits managed gene expression in a variety of numerous species. To facilitate the event of genetics techniques in a quick, straightforward, and standardized method, we constructed and examined a plasmid meeting toolbox that may allow the identification of well-regulated promoters in lots of Proteobacteria and doubtlessly past. Every plasmid consists of 4 classes of genetic components (i) the origin of replication, (ii) resistance marker, (iii) promoter-regulator and (iv) reporter.

The plasmids might be effectively assembled utilizing ligation-independent cloning, and any gene of curiosity might be simply inserted instead of the reporter. We examined this toolbox in 9 totally different Proteobacteria and recognized regulated promoters with over fifty-fold induction vary in eight of those micro organism. We additionally constructed variant libraries that enabled the identification of promoter-regulators with diversified expression ranges and elevated inducible fold change relative to the unique promoter. A number of over 50 plasmids, which include the entire toolbox’s genetic components, can be found for neighborhood use and can allow straightforward building and testing of genetics techniques in each mannequin and non-model micro organism.

 

Genetic reprogramming of wire blood derived endothelial colony forming cells in direction of human induced pluripotent stem cells utilizing episomal plasmids

 

Goal: This research aimed to isolate human umbilical wire blood derived endothelial colony forming cells (ECFCs) adopted by their integration free reprogramming in direction of induced pluripotent stem cells (iPSCs) and molecular characterization of each cell sorts utilizing multicolour flowcytometery and immunofluorescence respectively.

Strategies: The wire blood was collected from 37-39 weeks of gestational ages after C-section ex-utero from Dow College Hospital. The ECFCs remoted after ficoll primarily based separation of wire blood mononuclear cells (CBMNCs) which on emergence characterised by way of move cytometry and reprogrammed in direction of induced pluripotent stem cells (iPSCs) utilizing episomal vectors, the iPSCs had been characterised utilizing immunofluorescence. The research was performed at Stem Cells and Regenerative lab, Dow Analysis Institute of Biotechnology and Biomedical Sciences, Dow College of well being sciences OJHA campus. The research time length was about one yr (October 2017-October 2018); research design was in vitro experimental. The pattern dimension of the research was n=3.

Outcomes: The remoted ECFCs had been evaluated utilizing flowcytometery which confirmed constructive expression for CD31, CD34, CD146 cell floor markers and adverse for CD90. The profitable reprogramming of ECFCs in direction of iPSCs was confirmed by immunofluorescence utilizing OCT-4 which is taken into account to be a grasp regulator of pluripotency.

Conclusions: To the perfect of our information this research was the primary try and integration free reprogramming of wire blood derived endothelial colony forming cells in direction of induced pluripotent stem utilizing episomal plasmids. Cells which have been remoted from wire blood and people which have been reprogrammed each have potential therapeutic purposes in regenerative medication.

Increasing the SiMPl Plasmid Toolbox for Use with Spectinomycin/Streptomycin

 

We not too long ago developed the SiMPl plasmid toolbox, which is constituted by pairs of plasmids, generically indicated as pSiMPlx_N and pSiMPlx_C, which might be stably maintained in Escherichia coli with a single antibiotic x. The strategy exploits the cut up intein gp41-1 to reconstitute the enzyme conferring resistance towards the antibiotic x, whereby every enzyme fragment is expressed from one of many plasmids within the pair. pSiMPl plasmids are at the moment accessible to be used with ampicillin, kanamycin, chloramphenicol, hygromycin, and puromycin. Right here, we introduce one other pair to be used with spectinomycin/streptomycin, broadening the appliance spectrum of the SiMPl toolbox. To search out purposeful splice websites in aminoglycoside adenylyltransferase, we apply a streamlined technique wanting solely on the flexibility of native cysteine and serine residues, which we first validated splitting the enzymes conferring resistance towards ampicillin, kanamycin, chloramphenicol, and hygromycin. This technique might be used sooner or later to separate different enzymes conferring resistance towards antibiotics.

heraeus-targets

heraeus-targets

To Plate or to Merely Unfreeze, That Is the Query for Optimum Plasmid Extraction

 

Many molecular biology purposes require quick plasmid DNA extraction, spurring a number of research on easy methods to pace up the method. It’s frequently instructed in normal laboratory protocols to plate out frozen glycerol bacterial shares previous to micro organism incubation in liquid media and subsequent plasmid extraction, though the rationale for that is usually unexplained (apart from for the isolation of single colonies). Given the commonality and significance of this laboratory operation, such a apply is time-consuming and laborious.

To check the impression of this apply and the choice direct culturing technique, we investigated the affiliation between bacterial cell mass and its potential affect on plasmid yields from the two strategies. Our outcomes confirmed no distinction with preplating for 7 out of eight plasmid constructs used within the research, suggesting that direct glycerol restoration wouldn’t result in poorer plasmid yields. The findings assist the rationale for direct glycerol restoration for plasmid extraction, with out the necessity of an intermediate preplating step.

The Ferric Citrate Uptake System Encoded in a Novel bla CTX-M-3– and bla TEM-1-Harboring Conjugative Plasmid Contributes to the Virulence of Escherichia coli

 

Escherichia coli is one main explanation for bacterial infections and might horizontally purchase antimicrobial resistance and virulence genes by way of conjugation. As a result of conjugative plasmids can quickly unfold amongst micro organism of various species, the plasmids carrying each antimicrobial resistance and virulence genes might pose a vital risk to public well being. Due to this fact, the identification and characterization of those plasmids might facilitate a greater understanding of E. coli pathogenesis and the event of recent methods in opposition to E. coli infections. As a result of iron uptake capability is a possible virulence trait of micro organism, we screened for E. coli conjugative plasmids capable of confer each iron uptake capability and ampicillin resistance. The plasmid pEC41, which was derived from the bacteremia medical isolate EC41, was recognized. EC41, which carried the fimHbla CTX-M-3 was initially recognized. pEC41 carried bla CTX-M-3 and bla TEM-1. The ferric citrate uptake (fec) system was recognized in pEC41 and was accountable for conferring iron uptake capability.

The fec system contributes to the pathogenesis of EC41 in systemic infections however not in urinary tract infections (UTIs). Nonetheless, this method promoted aggressive health of a cystitis-associated medical isolate to colonize urinary tracts. Moreover, the distribution of the fec system was associated to E. coli isolates related to human bacteremia and UTIs. In abstract, the current research recognized a novel conjugative plasmid, pEC41, which conferred each antimicrobial resistance and an additional iron uptake capability to E. coli. The iron uptake capability was encoded within the fec system and contributed to E. coli pathogenesis. This research is the primary to indicate that the fec system is a virulence think about E. coli.

 

TransferTip ES cell transfer
E5195000079 PK25
EUR 727.2
Monoclonal Anti-Poly-His (6xhis) (His-tag) IgG
HISP14-M 100 ug
EUR 578.4
100µL Transfer Pipettes
TP100 N/A
EUR 15
Description: For pipetting 100µL samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, disposable, easy-to-use. Quantity: 10 pipettes.
1000µL Transfer Pipettes
TP1000 N/A
EUR 15
Description: For pipetting 1000µL samples. To be used with the Histamine Quick Test Strips. Key Features: Single use, disposable, easy-to-use. Quantity: 10 pipettes.
20µL Transfer Pipettes
TP20 N/A
EUR 15
Description: For pipetting 20µL samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, disposable, easy-to-use. Quantity: 10 pipettes.
200µL Transfer Pipettes
TP200 N/A
EUR 15
Description: For pipetting 200µL samples. To be used with the Histamine Quick Test Strips. Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.
300µL Transfer Pipettes
TP300 N/A
EUR 15
Description: For pipetting 300µL samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.
400µL Transfer Pipettes
TP400 N/A
EUR 15
Description: For pipetting 400µL samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.
50µL Transfer Pipettes
TP50 N/A
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Propane Transfer System
M-CEM-37015 1 UNIT
EUR 688
Description: Propane Transfer System
Thermal Transfer Ribbon
HIS3233 EACH
EUR 181.2
Transfer Rack 0.5 mL
E3880000305 EACH
EUR 52.8
Goat Anti-Poly-His (6xhis) (His-tag) IgG, aff pure
HISP16-A 100 ug
EUR 489.6
Rat cholest erolester transfer protein/lipid transfer protein ELISA kit
E02C0732-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat cholest erolester transfer protein/lipid transfer protein ELISA kit
E02C0732-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat cholest erolester transfer protein/lipid transfer protein ELISA kit
E02C0732-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat cholest erolester transfer protein/lipid transfer protein ELISA kit
E06C0732-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat cholest erolester transfer protein/lipid transfer protein ELISA kit
E06C0732-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat cholest erolester transfer protein/lipid transfer protein ELISA kit
E06C0732-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog cholest erolester transfer protein/lipid transfer protein ELISA kit
E08C0732-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog cholest erolester transfer protein/lipid transfer protein ELISA kit
E08C0732-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog cholest erolester transfer protein/lipid transfer protein ELISA kit
E08C0732-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E07C0732-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E07C0732-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E07C0732-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human cholest erolester transfer protein/lipid transfer protein ELISA kit
E01C0732-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human cholest erolester transfer protein/lipid transfer protein ELISA kit
E01C0732-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human cholest erolester transfer protein/lipid transfer protein ELISA kit
E01C0732-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit
E03C0732-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit
E03C0732-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit
E03C0732-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit
E04C0732-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit
E04C0732-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit
E04C0732-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit
E09C0732-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit
E09C0732-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit
E09C0732-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Phospholipid transfer Antibody
20-abx110096
  • EUR 493.2
  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
SPINE Puck Transfer Tool
M-SP-PTT-1 1 UNIT
EUR 386
Description: SPINE Puck Transfer Tool
Monoclonal Anti-Poly-His (6xhis) (His-tag) antibody, ascites
HISP11-M 100 ul
EUR 578.4
Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E05C0732-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E05C0732-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E05C0732-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
6400 Thermal Transfer Ribbon
804777 EACH
EUR 45.72
pET-(-30)GFP-9xGGS-Cre-6xHis Plasmid
PVT17107 2 ug
EUR 390
Transfer Rack 1.5/2.0 mL
E3880000151 EACH
EUR 49.2
Goat Anti-Poly-His (6xhis) (His-tag) IgG-FITC conjugate
HISP16-FITC 100 ul
EUR 416.4
SSRL Cassette Transfer Handle
M-CP-111-003 1 UNIT
EUR 460
Description: SSRL Cassette Transfer Handle
Human cholest erolester transfer protein/lipid transfer protein,CETP/LTP ELISA Kit
201-12-1516 96 tests
EUR 528
  • This cholest erolester transfer protein/lipid transfer protein ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit
GA-E1532HM-48T 48T
EUR 346.8
Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit
GA-E1532HM-96T 96T
EUR 559.2
Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit
QY-E03917 96T
EUR 433.2
Human cholest erolester transfer protein/lipid transfer protein,CETP/LTP ELISA Kit
YLA0018HU-48T 48T
EUR 435
Human cholest erolester transfer protein/lipid transfer protein,CETP/LTP ELISA Kit
YLA0018HU-96T 96T
EUR 562.5
Cryo Transfer Face for SEM
M-CEM-37000-S 1 UNIT
EUR 3295
Description: Cryo Transfer Face for SEM
Cryo Transfer Face for TEM
M-CEM-37000-T 1 UNIT
EUR 3185
Description: Cryo Transfer Face for TEM
Rabbit Anti-Poly-His (6xhis) (His-tag) IgG-IR700DX conjugate
HISP17-IR7 100 ul
EUR 489.6
Phospholipid transfer Antibody (HRP)
20-abx108612
  • EUR 493.2
  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Phospholipid transfer Antibody (FITC)
20-abx107192
  • EUR 493.2
  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Laboratory Thermal Transfer Printer
BAL4730 EACH
EUR 1458
BioPhotometer data transfer software
E6132854007 EACH
EUR 464.4
Thermal Transfer Ribbon 1x2.7 OD
HIS3524 EACH
EUR 244.8
Phospholipid transfer Antibody (Biotin)
20-abx105774
  • EUR 493.2
  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Human Transfer factor ELISA Kit
ELA-E1333h 96 Tests
EUR 988.8
Transfer Pipette 15mL Large Bulb
ARG1836 PK250
EUR 34.34
Xpert Native Transfer Buffer(10X)
P9001-050 500ml
EUR 111.6
Xpert Native Transfer Buffer(10X)
P9001-100 1L
EUR 165.6
Phospholipid transfer Polyclonal Antibody
42297-100ul 100ul
EUR 399.6
PAGE Gel Transfer Buffer, 10 x
GR103018 1 L
EUR 79
SuperBlot Western Transfer buffer 10X
DG-WEST 500ml
EUR 136.56
  • Product category: Biochemicals/Biological Buffers/Protein Related
Bovine Glycolipid transfer protein (GLTP)
1-CSB-YP009539BO
  • EUR 814.8
  • EUR 402
  • EUR 2606.4
  • EUR 1261.2
  • EUR 1730.4
  • EUR 522
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 25.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Bovine Glycolipid transfer protein(GLTP) expressed in Yeast
Bovine Glycolipid transfer protein (GLTP)
1-CSB-EP009539BO
  • EUR 733.2
  • EUR 370.8
  • EUR 2192.4
  • EUR 1126.8
  • EUR 1461.6
  • EUR 476.4
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 39.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Bovine Glycolipid transfer protein(GLTP) expressed in E.coli
Human Transfer factor,TF ELISA Kit
201-12-1733 96 tests
EUR 528
  • This Transfer factor ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Human Transfer factor,TF ELISA Kit
CN-04584H1 96T
EUR 520.8
Human Transfer factor,TF ELISA Kit
CN-04584H2 48T
EUR 340.8
Human Transfer factor(TF)ELISA Kit
GA-E1749HM-48T 48T
EUR 346.8
Human Transfer factor(TF)ELISA Kit
GA-E1749HM-96T 96T
EUR 559.2
Human Transfer factor(TF)ELISA Kit
QY-E00085 96T
EUR 472.8
Human Transfer factor,TF ELISA Kit
YLA1849HU-48T 48T
EUR 435
Human Transfer factor,TF ELISA Kit
YLA1849HU-96T 96T
EUR 562.5
Human Phospholipid transfer protein (PLTP)
1-CSB-YP018212HU
  • EUR 516
  • EUR 280.8
  • EUR 1809.6
  • EUR 770.4
  • EUR 1210.8
  • EUR 349.2
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 55.1 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Phospholipid transfer protein(PLTP) expressed in Yeast
Human Phospholipid transfer protein (PLTP)
1-CSB-EP018212HU
  • EUR 456
  • EUR 256.8
  • EUR 1570.8
  • EUR 672
  • EUR 1047.6
  • EUR 314.4
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 80.1 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Phospholipid transfer protein(PLTP) expressed in E.coli
Glycolipid Transfer Protein (GLTP) Protein
20-abx262570
  • EUR 393.6
  • EUR 7676.4
  • EUR 276
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.
Glycolipid Transfer Protein (GLTP) Antibody
abx233495-100ug 100 ug
EUR 610.8
  • Shipped within 5-12 working days.
Glycolipid Transfer Protein (GLTP) Antibody
20-abx112809
  • EUR 878.4
  • EUR 477.6
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.
Glycolipid Transfer Protein (GLTP) Antibody
20-abx128414
  • EUR 510
  • EUR 159.6
  • EUR 1446
  • EUR 693.6
  • EUR 393.6
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
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