To Plate or to Simply Unfreeze, That Is the Question for Optimal Plasmid Extraction

heraeus-targets

Many molecular biology purposes require quick plasmid DNA extraction, spurring a number of research on easy methods to pace up the method. It’s frequently instructed in normal laboratory protocols to plate out frozen glycerol bacterial shares previous to micro organism incubation in liquid media and subsequent plasmid extraction, though the rationale for that is usually unexplained (apart from for the isolation of single colonies). Given the commonality and significance of this laboratory operation, such a apply is time-consuming and laborious.

To check the impression of this apply and the choice direct culturing technique, we investigated the affiliation between bacterial cell mass and its potential affect on plasmid yields from the two strategies. Our outcomes confirmed no distinction with preplating for 7 out of eight plasmid constructs used within the research, suggesting that direct glycerol restoration would not result in poorer plasmid yields. The findings assist the rationale for direct glycerol restoration for plasmid extraction, with out the necessity of an intermediate preplating step.

 

plasmid toolbox for managed gene expression throughout the Proteobacteria

 

Managed gene expression is prime for the research of gene operate and our capability to engineer micro organism. Nonetheless, there’s at the moment no easy-to-use genetics toolbox that permits managed gene expression in a variety of numerous species. To facilitate the event of genetics techniques in a quick, straightforward, and standardized method, we constructed and examined a plasmid meeting toolbox that may allow the identification of well-regulated promoters in lots of Proteobacteria and doubtlessly past. Every plasmid consists of 4 classes of genetic components (i) the origin of replication, (ii) resistance marker, (iii) promoter-regulator and (iv) reporter.

The plasmids might be effectively assembled utilizing ligation-independent cloning, and any gene of curiosity might be simply inserted instead of the reporter. We examined this toolbox in 9 totally different Proteobacteria and recognized regulated promoters with over fifty-fold induction vary in eight of those micro organism. We additionally constructed variant libraries that enabled the identification of promoter-regulators with diversified expression ranges and elevated inducible fold change relative to the unique promoter. A number of over 50 plasmids, which include the entire toolbox’s genetic components, can be found for neighborhood use and can allow straightforward building and testing of genetics techniques in each mannequin and non-model micro organism.

 

Genetic reprogramming of wire blood derived endothelial colony forming cells in direction of human induced pluripotent stem cells utilizing episomal plasmids

 

Goal: This research aimed to isolate human umbilical wire blood derived endothelial colony forming cells (ECFCs) adopted by their integration free reprogramming in direction of induced pluripotent stem cells (iPSCs) and molecular characterization of each cell sorts utilizing multicolour flowcytometery and immunofluorescence respectively.

Strategies: The wire blood was collected from 37-39 weeks of gestational ages after C-section ex-utero from Dow College Hospital. The ECFCs remoted after ficoll primarily based separation of wire blood mononuclear cells (CBMNCs) which on emergence characterised by way of move cytometry and reprogrammed in direction of induced pluripotent stem cells (iPSCs) utilizing episomal vectors, the iPSCs had been characterised utilizing immunofluorescence. The research was performed at Stem Cells and Regenerative lab, Dow Analysis Institute of Biotechnology and Biomedical Sciences, Dow College of well being sciences OJHA campus. The research time length was about one yr (October 2017-October 2018); research design was in vitro experimental. The pattern dimension of the research was n=3.

Outcomes: The remoted ECFCs had been evaluated utilizing flowcytometery which confirmed constructive expression for CD31, CD34, CD146 cell floor markers and adverse for CD90. The profitable reprogramming of ECFCs in direction of iPSCs was confirmed by immunofluorescence utilizing OCT-4 which is taken into account to be a grasp regulator of pluripotency.

Conclusions: To the perfect of our information this research was the primary try and integration free reprogramming of wire blood derived endothelial colony forming cells in direction of induced pluripotent stem utilizing episomal plasmids. Cells which have been remoted from wire blood and people which have been reprogrammed each have potential therapeutic purposes in regenerative medication.

Increasing the SiMPl Plasmid Toolbox for Use with Spectinomycin/Streptomycin

 

We not too long ago developed the SiMPl plasmid toolbox, which is constituted by pairs of plasmids, generically indicated as pSiMPlx_N and pSiMPlx_C, which might be stably maintained in Escherichia coli with a single antibiotic x. The strategy exploits the cut up intein gp41-1 to reconstitute the enzyme conferring resistance towards the antibiotic x, whereby every enzyme fragment is expressed from one of many plasmids within the pair. pSiMPl plasmids are at the moment accessible to be used with ampicillin, kanamycin, chloramphenicol, hygromycin, and puromycin. Right here, we introduce one other pair to be used with spectinomycin/streptomycin, broadening the appliance spectrum of the SiMPl toolbox. To search out purposeful splice websites in aminoglycoside adenylyltransferase, we apply a streamlined technique wanting solely on the flexibility of native cysteine and serine residues, which we first validated splitting the enzymes conferring resistance towards ampicillin, kanamycin, chloramphenicol, and hygromycin. This technique might be used sooner or later to separate different enzymes conferring resistance towards antibiotics.

heraeus-targets

heraeus-targets

To Plate or to Merely Unfreeze, That Is the Query for Optimum Plasmid Extraction

 

Many molecular biology purposes require quick plasmid DNA extraction, spurring a number of research on easy methods to pace up the method. It’s frequently instructed in normal laboratory protocols to plate out frozen glycerol bacterial shares previous to micro organism incubation in liquid media and subsequent plasmid extraction, though the rationale for that is usually unexplained (apart from for the isolation of single colonies). Given the commonality and significance of this laboratory operation, such a apply is time-consuming and laborious.

To check the impression of this apply and the choice direct culturing technique, we investigated the affiliation between bacterial cell mass and its potential affect on plasmid yields from the two strategies. Our outcomes confirmed no distinction with preplating for 7 out of eight plasmid constructs used within the research, suggesting that direct glycerol restoration wouldn’t result in poorer plasmid yields. The findings assist the rationale for direct glycerol restoration for plasmid extraction, with out the necessity of an intermediate preplating step.

The Ferric Citrate Uptake System Encoded in a Novel bla CTX-M-3– and bla TEM-1-Harboring Conjugative Plasmid Contributes to the Virulence of Escherichia coli

 

Escherichia coli is one main explanation for bacterial infections and might horizontally purchase antimicrobial resistance and virulence genes by way of conjugation. As a result of conjugative plasmids can quickly unfold amongst micro organism of various species, the plasmids carrying each antimicrobial resistance and virulence genes might pose a vital risk to public well being. Due to this fact, the identification and characterization of those plasmids might facilitate a greater understanding of E. coli pathogenesis and the event of recent methods in opposition to E. coli infections. As a result of iron uptake capability is a possible virulence trait of micro organism, we screened for E. coli conjugative plasmids capable of confer each iron uptake capability and ampicillin resistance. The plasmid pEC41, which was derived from the bacteremia medical isolate EC41, was recognized. EC41, which carried the fimHbla CTX-M-3 was initially recognized. pEC41 carried bla CTX-M-3 and bla TEM-1. The ferric citrate uptake (fec) system was recognized in pEC41 and was accountable for conferring iron uptake capability.

The fec system contributes to the pathogenesis of EC41 in systemic infections however not in urinary tract infections (UTIs). Nonetheless, this method promoted aggressive health of a cystitis-associated medical isolate to colonize urinary tracts. Moreover, the distribution of the fec system was associated to E. coli isolates related to human bacteremia and UTIs. In abstract, the current research recognized a novel conjugative plasmid, pEC41, which conferred each antimicrobial resistance and an additional iron uptake capability to E. coli. The iron uptake capability was encoded within the fec system and contributed to E. coli pathogenesis. This research is the primary to indicate that the fec system is a virulence think about E. coli.

 

Recombinant Thermobifida Fusca Cutinase(C-6xHis)
CR10-1mg 1mg
EUR 2486
Description: Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl,1mM 2-mercaptoethanol,2mM MnCl2,150mM NaCl,pH7.5.
Recombinant Thermobifida Fusca Cutinase(C-6xHis)
CR10-500ug 500ug
EUR 1755
Description: Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl,1mM 2-mercaptoethanol,2mM MnCl2,150mM NaCl,pH7.5.
Recombinant Thermobifida Fusca Cutinase(C-6xHis)
CR10-50ug 50ug
EUR 496
Description: Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl,1mM 2-mercaptoethanol,2mM MnCl2,150mM NaCl,pH7.5.
pET- NLS- Cas9- 6xHis
PVT10639 2 ug
EUR 301
pET-dCas9-VP64-6xHis
PVT17702 2 ug
EUR 300
pET-6xHis-(-30)GFP
PVT17881 2 ug
EUR 341
pLenti-CARM1 shRNA-2 Plasmid
PVTBAV03691-2 2 ug
EUR 356
pLenti-CTLA4 shRNA-2 Plasmid
PVTBAV05689-2 2 ug
EUR 356
pLenti-FOXM1 shRNA-2 Plasmid
PVTBAV08732-2 2 ug
EUR 356
pLenti-JUN shRNA-2 Plasmid
PVTBAV11741-2 2 ug
EUR 356
pLenti-LHX6 shRNA-2 Plasmid
PVTBAV12881-2 2 ug
EUR 356
pLenti-MAGEA3 shRNA-2 Plasmid
PVTBAV13661-2 2 ug
EUR 356
pLenti-RUNX3 shRNA-2 Plasmid
PVTBAV20583-2 2 ug
EUR 356
pLenti-Slc7a11 shRNA-2 Plasmid
PVTBAV21973-2 2 ug
EUR 356
pLenti-STAT3 shRNA-2 Plasmid
PVTBAV22921-2 2 ug
EUR 356
pLenti-XRCC5 shRNA-2 Plasmid
PVTBAV26238-2 2 ug
EUR 356
Mouse Anti-6xHIS Tag monoclonal antibody
CABT-BL8767 100ug
EUR 559
pDONR223-CD73 Plasmid
PVTB00480-2 2 ug
EUR 356
Multi Fusion-Tagged recombinant Protein 52-Kda containing 16-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, Flag, S-tag, HA, KT3, E2, Au1, Au5, 6xHis-tags) for ELISA/Western
MFPM52-C 100 ul
EUR 286
Plasmid Midi Kit I
K1314-2
EUR 262
Plasmid Midi Kit II
K1315-2
EUR 262
Plasmid ezFilter Mega3 Kit
K1320-2
EUR 343
Plasmid ezFilter Mega6 Kit
K1321-2
EUR 370
Plasmid ezFilter Mega10 Kit
K1322-2
EUR 452
pCR4-TOPO-RNF135 Plasmid
PVTB01189-2 2 ug
EUR 356
pGEM-Ltf(Q25R) Plasmid
PVTB50084-2 2 ug
EUR 356
Monoclonal Anti-Poly-His (6xhis) (His-tag) IgG
HISP14-M 100 ug
EUR 482
ToxOut? Endofree Plasmid Midi Kit
K1328-2
EUR 207
ToxOut? Endofree Plasmid Maxi Kit
K1330-2
EUR 234
ToxOut? Endofree Plasmid Mega3 Kit
K1332-2
EUR 316
ToxOut? Endofree Plasmid Mega6 Kit
K1333-2
EUR 370
ToxOut? Endofree Plasmid Mega10 Kit
K1334-2
EUR 425
pMD19-T-LEO1(E225G) Plasmid
PVTB01141-2 2 ug
EUR 356
Plasmid ezFilter Midi Kit I, Centrifuge
K1316-2
EUR 262
Plasmid ezFilter Midi Kit II, Centrifuge
K1317-2
EUR 262
Express Plasmid Midiprep Kit (25 min)
K1323-2
EUR 262
Express Plasmid Maxiprep Kit (25 min)
K1324-2
EUR 283
ToxOut? Endofree ezFilter Plasmid Midi Kit
K1329-2
EUR 207
ToxOut? Endofree ezfilter Plasmid Maxi Kit
K1331-2
EUR 234
ToxOut? Express Endofree Plasmid Midi Kit
K1335-2
EUR 234
ToxOut? Express Endofree Plasmid Maxi Kit
K1336-2
EUR 262
pCDH-EF1-CymR-T2A-Puro (plasmid)
QM200PA-2 10 ug
EUR 597
  • Category: Lentiviral Technology
pCDH-EF1-CymR-T2A-Neo (plasmid)
QM400PA-2 10 ug
EUR 581
  • Category: Lentiviral Technology
Phospholipid transfer Antibody
20-abx110096
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Monoclonal Anti-Poly-His (6xhis) (His-tag) antibody, ascites
HISP11-M 100 ul
EUR 482
Melanoma; Clone HMB45 (Concentrate)
A00019-C.2 0.2 ml
EUR 236
2-Propyl-β-D-glucuronide, 2 mg
0904-2 2 mg
EUR 162
HER-2 / c-erbB-2 / neu / CD340; Clone HRB2/451 (Concentrate)
RA0108-C.1 0.1 ml
EUR 125
HER-2 / c-erbB-2 / neu / CD340; Clone HRB2/451 (Concentrate)
RA0108-C.5 0.5 ml
EUR 300
Mitomycin C
2713-2
EUR 120
Goat Anti-Poly-His (6xhis) (His-tag) IgG, aff pure
HISP16-A 100 ug
EUR 408
Goat Anti-Poly-His (6xhis) (His-tag) IgG-FITC conjugate
HISP16-FITC 100 ul
EUR 347
Rabbit Anti-Poly-His (6xhis) (His-tag) IgG-IR700DX conjugate
HISP17-IR7 100 ul
EUR 408
IL-2 Interleukin-2 Human Recombinant Protein, His Tag
PROTP60568-2 Regular: 10ug
EUR 317
Description: Interleukin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 133 amino acids fragment (21-153) having a molecular weight of 20kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. _x000D_ The IL-2 His is purified by proprietary chromatographic techniques._x000D_
Rat cholest erolester transfer protein/lipid transfer protein ELISA kit
E02C0732-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit
E03C0732-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit
E03C0732-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit
E03C0732-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog cholest erolester transfer protein/lipid transfer protein ELISA kit
E08C0732-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog cholest erolester transfer protein/lipid transfer protein ELISA kit
E08C0732-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog cholest erolester transfer protein/lipid transfer protein ELISA kit
E08C0732-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat cholest erolester transfer protein/lipid transfer protein ELISA kit
E06C0732-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat cholest erolester transfer protein/lipid transfer protein ELISA kit
E06C0732-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat cholest erolester transfer protein/lipid transfer protein ELISA kit
E06C0732-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit
E04C0732-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit
E04C0732-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit
E04C0732-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E07C0732-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E07C0732-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E07C0732-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat cholest erolester transfer protein/lipid transfer protein ELISA kit
E02C0732-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat cholest erolester transfer protein/lipid transfer protein ELISA kit
E02C0732-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human cholest erolester transfer protein/lipid transfer protein ELISA kit
E01C0732-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human cholest erolester transfer protein/lipid transfer protein ELISA kit
E01C0732-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human cholest erolester transfer protein/lipid transfer protein ELISA kit
E01C0732-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit
E09C0732-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit
E09C0732-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit
E09C0732-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
BMP-2 Bone Morphogenetic Protein-2 Human Recombinant Protein, Monomer
PROTP12643-2 Regular: 20ug
EUR 317
Description: Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 115 amino acids (283-396) and having a molecular mass of 13009 Dalton. ;The BMP-2 is purified by proprietary chromatographic techniques.
PG LIVER 2 EA*
59423-2 2 EA
EUR 192.24
Individual Reaction Mix 2
G065-2 200 reactions
EUR 167
Phospholipid transfer Antibody (Biotin)
20-abx105774
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Phospholipid transfer Antibody (FITC)
20-abx107192
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Phospholipid transfer Antibody (HRP)
20-abx108612
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Phospholipid transfer Polyclonal Antibody
42297-100ul 100ul
EUR 333
pCMV-C-HA-Bcl-2 Plasmid
PVTB00307-2c 2 ug
EUR 356

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