Methyl fresh

MethylFlash Methylated DNA 5-mC

Principle & Procedure

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at 450 nm.

The amount of methylated DNA is proportional to the OD intensity measured, which can be calculated with the kit’s included formulas for relative methylation status of two different DNA samples or absolute quantification of 5-methylcytosine (5mC) using a standard curve.

Product Overview
We recommend using a newer version of this product: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in an ELISA-like microplate-based format. Compared to LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash  technology directly quantifies current global DNA methylation.

This kit is also specifically optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.

Product Features

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a further refinement of our previous Methylamp ™ global DNA methylation quantification kits by simplifying the workflow and improving the consistency of results. It uses an innovative method to enable background signals to be extremely low, eliminating the plate drying and blocking steps. Compared to chromatography-based methods such as HPLC or mass spectrometry, this product provides a cost-effective way to accurately measure levels of 5-methylcytosine. The kit has the following advantages and features:

Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.
High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA and accepts as low as 50 ng of input genomic DNA.
Optimized antibody and enhancer solutions allow high specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.
Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.
Strip-well microplate format makes the assay flexible: manual or high throughput analysis.

Easy, Fast, and Flexible

The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 4 hours. The strip-well microplate format allows for a flexible assay in manual or high throughput analysis. Universal positive and negative controls are included with the kit for quantifying methylated DNA from any species such as mammals, plants, fungi, bacteria, and viruses.

methylflash methylated dna5-mc quantification kit

methylflash methylated dna5-mc quantification kit

Safe and Convenient

All the needed reagents, including negative controls and positive controls, for quantification of global DNA methylation are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for DNA digestion / denaturation, radioactivity, extraction, or chromatography.

Highly Sensitive and Specific
The novel procedure and proprietary kit compositions allow for accurate quantification of methylated DNA to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.2 ng of methylated DNA.

 

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D6334149 5 ug Ask for price
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Methylated DNA Control

D6434149 5 ug Ask for price
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

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Description: Ask the seller for details

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Monkey (Rhesus) Methylated DNA Control

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Monkey (Cynomolgus) Methylated DNA Control

D6534149-Cy 5 ug Ask for price
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D6234148-PP 2x5 ug
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Methylated & Low-Methylated DNA Matching Pair - Human Lymphocyte cell line

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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)

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EUR 904.05
Description: reagents widely cited

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  • 100 ug
  • 1 mg
  • 200 ug
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Methylated-DNA--protein-cysteine methyltransferase Polyclonal Antibody

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1 KB DNA LADDER, 500UL PER KIT

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MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

P-1030 96 reactions
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Description: Ask the seller for details

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)

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ELISA kit for Human Methylated-DNA--protein-cysteine methyltransferase

EK4201 96 tests
EUR 553
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ELISA kit for Dog Methylated-DNA--protein-cysteine methyltransferase

EK4202 96 tests
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Description: Enzyme-linked immunosorbent assay kit for quantification of Dog Methylated-DNA--protein-cysteine methyltransferase in samples from serum, plasma, tissue homogenates and other biological fluids.

Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit

EH2056 96T
EUR 567.6
  • Detection range: 0.156-10 ng/ml
  • Uniprot ID: P16455
  • Alias: MGMT(Methylated-DNA--protein-cysteine methyltransferase)/6-O-methylguanine-DNA methyltransferase/MGMT/O-6-methylguanine-DNA-alkyltransferase
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

ELISA kit for Human Methylated-DNA--protein-cysteine methyltransferase (MGMT)

KTE62232-48T 48T
EUR 332
  • O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens because of its preference for pairing with thymine during DNA replication. This adduct in DNA is removed by a ubiquitous and unique repair p
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Description: Quantitative sandwich ELISA for measuring Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Methylated-DNA--protein-cysteine methyltransferase (MGMT)

KTE62232-5platesof96wells 5 plates of 96 wells
EUR 2115
  • O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens because of its preference for pairing with thymine during DNA replication. This adduct in DNA is removed by a ubiquitous and unique repair p
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Methylated-DNA--protein-cysteine methyltransferase (MGMT)

KTE62232-96T 96T
EUR 539
  • O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens because of its preference for pairing with thymine during DNA replication. This adduct in DNA is removed by a ubiquitous and unique repair p
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Methylated Lysine Antibody

abx448445-100ug 100 ug
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abx448362-100ug 100 ug
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Methylated Lysine Antibody (FITC)

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Methylated Lysine Antibody (RPE)

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Methylated Lysine Antibody (Streptavidin)

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  • Shipped within 5-12 working days.

Methylated Lysine Antibody (Biotin)

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  • Shipped within 5-12 working days.

Methylated Lysine Antibody (HRP)

abx448447-400ul 400 ul
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Pan Methylated Lysine Antibody

abx217631-100ug 100 ug
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  • Shipped within 5-10 working days.