MethylFlash Methylated DNA 5-mC

Principle & Procedure

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at 450 nm.

The amount of methylated DNA is proportional to the OD intensity measured, which can be calculated with the kit’s included formulas for relative methylation status of two different DNA samples or absolute quantification of 5-methylcytosine (5mC) using a standard curve.

Product Overview
We recommend using a newer version of this product: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in an ELISA-like microplate-based format. Compared to LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash  technology directly quantifies current global DNA methylation.

This kit is also specifically optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.

Product Features

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a further refinement of our previous Methylamp ™ global DNA methylation quantification kits by simplifying the workflow and improving the consistency of results. It uses an innovative method to enable background signals to be extremely low, eliminating the plate drying and blocking steps. Compared to chromatography-based methods such as HPLC or mass spectrometry, this product provides a cost-effective way to accurately measure levels of 5-methylcytosine. The kit has the following advantages and features:

Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.
High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA and accepts as low as 50 ng of input genomic DNA.
Optimized antibody and enhancer solutions allow high specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.
Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.
Strip-well microplate format makes the assay flexible: manual or high throughput analysis.

Easy, Fast, and Flexible

The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 4 hours. The strip-well microplate format allows for a flexible assay in manual or high throughput analysis. Universal positive and negative controls are included with the kit for quantifying methylated DNA from any species such as mammals, plants, fungi, bacteria, and viruses.

methylflash methylated dna5-mc quantification kit

Safe and Convenient

All the needed reagents, including negative controls and positive controls, for quantification of global DNA methylation are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for DNA digestion / denaturation, radioactivity, extraction, or chromatography.

Highly Sensitive and Specific
The novel procedure and proprietary kit compositions allow for accurate quantification of methylated DNA to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.2 ng of methylated DNA.

MethylFlash 5-mC RNA Methylation ELISA Easy Kit (Fluorometric)
P-9009	EpiGentek	EUR 595.92 EUR 387.2 EUR 639.1	48 reactions 48 reactions 96 reactions

MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric)
P-1037	EpiGentek	EUR 1137.06 EUR 463.1 EUR 775.5	96 Assays 48 Assays 96 Assays

Rat Methylated DNA Control
D6434149	Biochain	5 ug	Ask for price

Human Methylated DNA Control
D6255815	Biochain	5 ug	EUR 128

Mouse Methylated DNA Control
D6334149	Biochain	5 ug	Ask for price

Methylamp Universal Methylated DNA Kit
P-1011	EpiGentek	EUR 684.66 EUR 292.6 EUR 442.2	20 Modifications 10 Modifications 20 Modifications

MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)
P-1036	EpiGentek	EUR 1084.86 EUR 424.6 EUR 738.1	96 Assays 48 Assays 96 Assays

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric)
P-1041	EpiGentek	EUR 1137.06 EUR 442.2 EUR 760.1	96 Assays 48 Assays 96 Assays

Methylamp Methylated DNA Capture (MeDIP) Kit
P-1015	EpiGentek	EUR 1050.06 EUR 424.6 EUR 712.8	48 Reactions 24 Reactions 48 Reactions

Monkey (Rhesus) Methylated DNA Control
D6534149	Biochain	5 ug	Ask for price

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)
P-1032	EpiGentek	EUR 1067.46 EUR 396 EUR 696.3	96 reactions 48 reactions 96 reactions

Methylamp Universal Methylated DNA Preparation Kit
P-1019	EpiGentek	40 µg	EUR 416.7

Monkey (Cynomolgus) Methylated DNA Control
D6534149-Cy	Biochain	5 ug	Ask for price

SuperSense Methylated DNA Quantification Kit
P-1021	EpiGentek	EUR 1137.06 EUR 442.2 EUR 760.1	96 Assays 48 Assays 96 Assays

EpiQuik Methylated DNA Immunoprecipitation Kit
P-2019	EpiGentek	EUR 1067.46 EUR 451 EUR 724.9	48 Reactions 24 Reactions 48 Reactions

Fully CpG Methylated Human Genomic DNA
X10000	EpiGentek	10 µg/100 µl	Ask for price

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)
P-1039	EpiGentek	EUR 945.66 EUR 360.8 EUR 625.9	96 Assays 48 Assays 96 Assays

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Fluorometric)
P-1040	EpiGentek	EUR 945.66 EUR 360.8 EUR 625.9	96 Assays 48 Assays 96 Assays

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit
P-2020	EpiGentek	EUR 1067.46 EUR 442.2 EUR 710.6	48 Reactions 24 Reactions 48 Reactions

Methylated-DNA--protein-cysteine methyltransferase Antibody
20-abx109997	Abbexa	EUR 493.2 EUR 2214 EUR 718.8 EUR 218.4 EUR 360	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

Methylated & Low-Methylated DNA Matched Pair - Human Lymphocyte cell line
D6254874-PP	Biochain	2x5 ug	EUR 351

MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)
P-9015	EpiGentek	EUR 945.66 EUR 380.6 EUR 625.9	96 Assays 48 Assays 96 Assays

Methylated-DNA--protein-cysteine methyltransferase Polyclonal Antibody
42551-100ul	SAB	100ul	EUR 399.6

Methylated & Low-Methylated DNA Matched Pair - Human Peripheral Blood Leukocyte
D6234148-PP	Biochain	2x5 ug	EUR 351

Methylated Lysine Antibody
abx448445-100ug	Abbexa	100 ug	EUR 644.4
Shipped within 5-12 working days.

ELISA kit for Dog Methylated-DNA--protein-cysteine methyltransferase
EK4202	SAB	96 tests	EUR 865.2
Description: Enzyme-linked immunosorbent assay kit for quantification of Dog Methylated-DNA--protein-cysteine methyltransferase in samples from serum, plasma, tissue homogenates and other biological fluids.

Dog MGMT/ Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
E0070Do	Sunlong	1 Kit	EUR 860.4

ELISA kit for Human Methylated-DNA--protein-cysteine methyltransferase
EK4201	SAB	96 tests	EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Methylated-DNA--protein-cysteine methyltransferase in samples from serum, plasma, tissue homogenates and other biological fluids.

Human MGMT/ Methylated-DNA--protein-cysteine methyltransferase ELISA Kit
E1588Hu	Sunlong	1 Kit	EUR 685.2

Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit
EH2056	FN Test	96T	EUR 681.12
Detection range: 0.156-10 ng/ml Uniprot ID: P16455 Alias: MGMT(Methylated-DNA--protein-cysteine methyltransferase)/6-O-methylguanine-DNA methyltransferase/MGMT/O-6-methylguanine-DNA-alkyltransferase
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Methylated-DNA--protein-cysteine methyltransferase Polyclonal Conjugated Antibody
C42551	SAB	100ul	EUR 476.4

Pan Methylated Lysine Antibody
abx217631-100ug	Abbexa	100 ug	EUR 526.8
Shipped within 5-10 working days.

Pan Methylated Lysine Antibody
20-abx159635	Abbexa	EUR 326.4 EUR 276	100 ug 50 ug
Shipped within 5-10 working days.

Methylated Lysine Antibody (APC)
abx448362-100ug	Abbexa	100 ug	EUR 710.4
Shipped within 5-12 working days.

Methylated Lysine Antibody (RPE)
abx448404-100ug	Abbexa	100 ug	EUR 710.4
Shipped within 5-12 working days.

Methylated Lysine Antibody (HRP)
abx448447-400ul	Abbexa	400 ul	EUR 727.2
Shipped within 5-12 working days.

Pan Methylated Lysine Antibody
DF7728	Affbiotech	200ul	EUR 420

Antibody for Methylated Lysine
SPC-158D	Stressmarq	0.1mg	EUR 434.4
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification o Show more
Description: A polyclonal antibody for Methylated Lysine from Species Independent. The antibody is produced in rabbit after immunization with Methylated KLH Conjugated. The Antibody is tested and validated for WB, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:20000), ICC/IF (1:200). This Methylated Lysine antibody is unconjugated.

Antibody for Methylated Lysine
SPC-158D-A390	Stressmarq	0.1mg	EUR 490.8
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification o Show more
Description: A polyclonal antibody for Methylated Lysine from Species Independent. The antibody is produced in rabbit after immunization with Methylated KLH Conjugated. The Antibody is tested and validated for WB, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:20000), ICC/IF (1:200). This Methylated Lysine antibody is conjugated to ATTO 390.

Antibody for Methylated Lysine
SPC-158D-A488	Stressmarq	0.1mg	EUR 489.6
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification o Show more
Description: A polyclonal antibody for Methylated Lysine from Species Independent. The antibody is produced in rabbit after immunization with Methylated KLH Conjugated. The Antibody is tested and validated for WB, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:20000), ICC/IF (1:200). This Methylated Lysine antibody is conjugated to ATTO 488.

Antibody for Methylated Lysine
SPC-158D-A565	Stressmarq	0.1mg	EUR 489.6
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification o Show more
Description: A polyclonal antibody for Methylated Lysine from Species Independent. The antibody is produced in rabbit after immunization with Methylated KLH Conjugated. The Antibody is tested and validated for WB, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:20000), ICC/IF (1:200). This Methylated Lysine antibody is conjugated to ATTO 565.