Methyl fresh

MethylFlash Methylated DNA 5-mC

Principle & Procedure

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at 450 nm.

The amount of methylated DNA is proportional to the OD intensity measured, which can be calculated with the kit’s included formulas for relative methylation status of two different DNA samples or absolute quantification of 5-methylcytosine (5mC) using a standard curve.

Product Overview
We recommend using a newer version of this product: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in an ELISA-like microplate-based format. Compared to LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash  technology directly quantifies current global DNA methylation.

This kit is also specifically optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.

Product Features

The MethylFlash  Methylated DNA Quantification Kit (Colorimetric) is a further refinement of our previous Methylamp ™ global DNA methylation quantification kits by simplifying the workflow and improving the consistency of results. It uses an innovative method to enable background signals to be extremely low, eliminating the plate drying and blocking steps. Compared to chromatography-based methods such as HPLC or mass spectrometry, this product provides a cost-effective way to accurately measure levels of 5-methylcytosine. The kit has the following advantages and features:

Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.
High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA and accepts as low as 50 ng of input genomic DNA.
Optimized antibody and enhancer solutions allow high specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.
Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.
Strip-well microplate format makes the assay flexible: manual or high throughput analysis.

Easy, Fast, and Flexible

The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 4 hours. The strip-well microplate format allows for a flexible assay in manual or high throughput analysis. Universal positive and negative controls are included with the kit for quantifying methylated DNA from any species such as mammals, plants, fungi, bacteria, and viruses.

methylflash methylated dna5-mc quantification kit

methylflash methylated dna5-mc quantification kit

Safe and Convenient

All the needed reagents, including negative controls and positive controls, for quantification of global DNA methylation are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for DNA digestion / denaturation, radioactivity, extraction, or chromatography.

Highly Sensitive and Specific
The novel procedure and proprietary kit compositions allow for accurate quantification of methylated DNA to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.2 ng of methylated DNA.

 

MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)

P-1034-48 48 Assays
EUR 387.2

MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)

P-1034-96 96 Assays
EUR 712.8

MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)

P-1034
  • EUR 1050.06
  • EUR 387.20
  • EUR 712.80
  • 96 Assays
  • 48 Assays
  • 96 Assays

Human Methylated DNA Control

D6255815 5 ug
EUR 187.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Mouse Methylated DNA Control

D6334149 5 ug Ask for price
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Methylated DNA Control

D6434149 5 ug Ask for price
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Rhesus) Methylated DNA Control

D6534149 5 ug Ask for price
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Cynomolgus) Methylated DNA Control

D6534149-Cy 5 ug Ask for price
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Methylamp Universal Methylated DNA Kit 

P-1011-1 10 Modifications
EUR 292.6

Methylamp Universal Methylated DNA Kit 

P-1011-2 20 Modifications
EUR 442.2

SuperSense Methylated DNA Quantification Kit

P-1021-48 48 Assays
EUR 442.2

SuperSense Methylated DNA Quantification Kit

P-1021-96 96 Assays
EUR 760.1

EpiQuik Methylated DNA Immunoprecipitation Kit 

P-2019-24 24 Reactions
EUR 451

EpiQuik Methylated DNA Immunoprecipitation Kit 

P-2019-48 48 Reactions
EUR 724.9

Methylamp Universal Methylated DNA Kit 

P-1011
  • EUR 684.66
  • EUR 292.60
  • EUR 442.20
  • 20 Modifications
  • 10 Modifications
  • 20 Modifications

SuperSense Methylated DNA Quantification Kit

P-1021
  • EUR 1137.06
  • EUR 442.20
  • EUR 760.10
  • 96 Assays
  • 48 Assays
  • 96 Assays

EpiQuik Methylated DNA Immunoprecipitation Kit 

P-2019
  • EUR 1067.46
  • EUR 451.00
  • EUR 724.90
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric) 

P-1037-48 48 Assays
EUR 463.1

MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric) 

P-1037-96 96 Assays
EUR 775.5

MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric) 

P-1037
  • EUR 1137.06
  • EUR 463.10
  • EUR 775.50
  • 96 Assays
  • 48 Assays
  • 96 Assays

Methylated & Low-Methylated DNA Matching Pair - Human Peripheral Blood Leukocyte

D6234148-PP 2x5 ug
EUR 411.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Methylated & Low-Methylated DNA Matching Pair - Human Lymphocyte cell line

D6254874-PP 2x5 ug
EUR 411.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Methylated-DNA--protein-cysteine methyltransferase Antibody

20-abx109997
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Methylamp Methylated DNA Capture (MeDIP) Kit 

P-1015-24 24 Reactions
EUR 424.6

Methylamp Methylated DNA Capture (MeDIP) Kit 

P-1015-48 48 Reactions
EUR 712.8

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit 

P-2020-24 24 Reactions
EUR 442.2

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit 

P-2020-48 48 Reactions
EUR 710.6

Methylamp Methylated DNA Capture (MeDIP) Kit 

P-1015
  • EUR 1050.06
  • EUR 424.60
  • EUR 712.80
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

Methylamp Universal Methylated DNA Preparation Kit

P-1019 40 µg
EUR 416.7

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit 

P-2020
  • EUR 1067.46
  • EUR 442.20
  • EUR 710.60
  • 48 Reactions
  • 24 Reactions
  • 48 Reactions

Fully CpG Methylated Human Genomic DNA

X10000 10 µg/100 µl Ask for price

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

Methylated-DNA--protein-cysteine methyltransferase Polyclonal Antibody

42551-100ul 100ul
EUR 399.6

MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)

P-1036-48 48 Assays
EUR 424.6

MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)

P-1036-96 96 Assays
EUR 738.1

MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)

P-1036
  • EUR 1084.86
  • EUR 424.60
  • EUR 738.10
  • 96 Assays
  • 48 Assays
  • 96 Assays

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) 

P-1041-48 48 Assays
EUR 442.2

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) 

P-1041-96 96 Assays
EUR 760.1

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) 

P-1041
  • EUR 1137.06
  • EUR 442.20
  • EUR 760.10
  • 96 Assays
  • 48 Assays
  • 96 Assays

Methylated-DNA--protein-cysteine methyltransferase Polyclonal Conjugated Antibody

C42551 100ul
EUR 476.4