Principle & Procedure
The MethylFlash Methylated DNA Quantification Kit (Colorimetric) contains all reagents necessary for the quantification of global DNA methylation. In this assay, DNA is bound to strip wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at 450 nm.
The amount of methylated DNA is proportional to the OD intensity measured, which can be calculated with the kit’s included formulas for relative methylation status of two different DNA samples or absolute quantification of 5-methylcytosine (5mC) using a standard curve.
Product Overview
We recommend using a newer version of this product: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
The MethylFlash Methylated DNA Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in an ELISA-like microplate-based format. Compared to LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash technology directly quantifies current global DNA methylation.
This kit is also specifically optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.
Product Features
The MethylFlash Methylated DNA Quantification Kit (Colorimetric) is a further refinement of our previous Methylamp ™ global DNA methylation quantification kits by simplifying the workflow and improving the consistency of results. It uses an innovative method to enable background signals to be extremely low, eliminating the plate drying and blocking steps. Compared to chromatography-based methods such as HPLC or mass spectrometry, this product provides a cost-effective way to accurately measure levels of 5-methylcytosine. The kit has the following advantages and features:
Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent.
High sensitivity, of which the detection limit can be as low as 0.2 ng of methylated DNA and accepts as low as 50 ng of input genomic DNA.
Optimized antibody and enhancer solutions allow high specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA.
Universal positive and negative controls are included, which are suitable for quantifying methylated DNA from any species.
Strip-well microplate format makes the assay flexible: manual or high throughput analysis.
Easy, Fast, and Flexible
The entire colorimetric assay has easy-to-follow steps for convenience and speed, which can be completed in 4 hours. The strip-well microplate format allows for a flexible assay in manual or high throughput analysis. Universal positive and negative controls are included with the kit for quantifying methylated DNA from any species such as mammals, plants, fungi, bacteria, and viruses.

methylflash methylated dna5-mc quantification kit
Safe and Convenient
All the needed reagents, including negative controls and positive controls, for quantification of global DNA methylation are conveniently packaged in the kit. The direct colorimetric quantification of DNA samples replaces obsolete or inferior methods and eliminates the need for DNA digestion / denaturation, radioactivity, extraction, or chromatography.
Highly Sensitive and Specific
The novel procedure and proprietary kit compositions allow for accurate quantification of methylated DNA to be achieved with high sensitivity and specificity. The detection limit of the input DNA can be as low as 0.2 ng of methylated DNA.
Mouse Methylated DNA Control |
D6334149 | Biochain | 5 ug | Ask for price |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Rat Methylated DNA Control |
D6434149 | Biochain | 5 ug | Ask for price |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric) |
P-1037 | EpiGentek | 96 Assays | EUR 947.55 |
Description: Ask the seller for details |
Monkey (Rhesus) Methylated DNA Control |
D6534149 | Biochain | 5 ug | Ask for price |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Monkey (Cynomolgus) Methylated DNA Control |
D6534149-Cy | Biochain | 5 ug | Ask for price |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Methylamp Universal Methylated DNA Kit |
P-1011 | EpiGentek | 20 Modifications | EUR 570.55 |
Description: kits suitable for this type of research |
SuperSense Methylated DNA Quantification Kit |
P-1021 | EpiGentek | 96 Assays | EUR 947.55 |
Description: fast delivery possible |
EpiQuik Methylated DNA Immunoprecipitation Kit |
P-2019 | EpiGentek | 48 Reactions | EUR 889.55 |
Description: Ask the seller for details |
Methylated & Low-Methylated DNA Matching Pair - Human Peripheral Blood Leukocyte |
D6234148-PP | Biochain | 2x5 ug | EUR 343 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Methylated & Low-Methylated DNA Matching Pair - Human Lymphocyte cell line |
D6254874-PP | Biochain | 2x5 ug | EUR 343 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Methylated-DNA--protein-cysteine methyltransferase Antibody |
20-abx109997 | Abbexa | - EUR 411.00
- EUR 1845.00
- EUR 599.00
- EUR 182.00
- EUR 300.00
| - 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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- Shipped within 5-10 working days.
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Fully CpG Methylated Human Genomic DNA |
X10000 | EpiGentek | 10 µg/100 µl | Ask for price |
Description: The best epigenetics products |
Methylamp Methylated DNA Capture (MeDIP) Kit |
P-1015 | EpiGentek | 48 Reactions | EUR 875.05 |
Description: Ask the seller for details |
Methylamp Universal Methylated DNA Preparation Kit |
P-1019 | EpiGentek | 40 µg | EUR 347.25 |
Description: The best epigenetics products |
EpiQuik Tissue Methylated DNA Immunoprecipitation Kit |
P-2020 | EpiGentek | 48 Reactions | EUR 889.55 |
Description: The best epigenetics products |
MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric) |
P-1036 | EpiGentek | 96 Assays | EUR 904.05 |
Description: reagents widely cited |
100 BP DNA LADDER, 500UL PER KIT |
M-DNA-100BP | CORNING | 1/pk | EUR 73 |
Description: Bioscience Mol Bio; DNA Ladder |
1 KB DNA LADDER, 500UL PER KIT |
M-DNA-1KB | CORNING | 1/pk | EUR 70 |
Description: Bioscience Mol Bio; DNA Ladder |
Methylated-DNA--protein-cysteine methyltransferase Polyclonal Antibody |
42551-100ul | SAB | 100ul | EUR 333 |
MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric) |
P-1041 | EpiGentek | 96 Assays | EUR 947.55 |
Description: kits suitable for this type of research |
Methylated-DNA--protein-cysteine methyltransferase Polyclonal Conjugated Antibody |
C42551 | SAB | 100ul | EUR 397 |
MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric) |
P-1030 | EpiGentek | 96 reactions | EUR 860.55 |
Description: Ask the seller for details |
MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) |
P-1032 | EpiGentek | 96 reactions | EUR 889.55 |
Description: The best epigenetics products |
Dog MGMT/ Methylated-DNA--protein-cysteine methyltransferase ELISA Kit |
E0070Do | Sunlong | 1 Kit | EUR 717 |
Human MGMT/ Methylated-DNA--protein-cysteine methyltransferase ELISA Kit |
E1588Hu | Sunlong | 1 Kit | EUR 571 |
ELISA kit for Human Methylated-DNA--protein-cysteine methyltransferase |
EK4201 | SAB | 96 tests | EUR 553 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Methylated-DNA--protein-cysteine methyltransferase in samples from serum, plasma, tissue homogenates and other biological fluids. |
ELISA kit for Dog Methylated-DNA--protein-cysteine methyltransferase |
EK4202 | SAB | 96 tests | EUR 721 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Dog Methylated-DNA--protein-cysteine methyltransferase in samples from serum, plasma, tissue homogenates and other biological fluids. |
Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit |
EH2056 | FN Test | 96T | EUR 567.6 |
- Detection range: 0.156-10 ng/ml
- Uniprot ID: P16455
- Alias: MGMT(Methylated-DNA--protein-cysteine methyltransferase)/6-O-methylguanine-DNA methyltransferase/MGMT/O-6-methylguanine-DNA-alkyltransferase
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
ELISA kit for Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) |
KTE62232-48T | Abbkine | 48T | EUR 332 |
- O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens because of its preference for pairing with thymine during DNA replication. This adduct in DNA is removed by a ubiquitous and unique repair p
- Show more
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Description: Quantitative sandwich ELISA for measuring Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) |
KTE62232-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2115 |
- O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens because of its preference for pairing with thymine during DNA replication. This adduct in DNA is removed by a ubiquitous and unique repair p
- Show more
|
Description: Quantitative sandwich ELISA for measuring Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) |
KTE62232-96T | Abbkine | 96T | EUR 539 |
- O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens because of its preference for pairing with thymine during DNA replication. This adduct in DNA is removed by a ubiquitous and unique repair p
- Show more
|
Description: Quantitative sandwich ELISA for measuring Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Pan Methylated Lysine |
DF7728 | Affbiotech | 200ul | EUR 304 |
Description: Pan Methylated Lysine Rabbit pAbdetects endogenous levels of total Pan Methylated Lysine Rabbit pAb. |
Methylated Lysine Antibody |
abx448445-100ug | Abbexa | 100 ug | EUR 537 |
- Shipped within 5-12 working days.
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Pan Methylated Lysine Antibody |
20-abx159635 | Abbexa | | |
- Shipped within 5-10 working days.
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Pan Methylated Lysine Antibody |
abx217631-100ug | Abbexa | 100 ug | EUR 439 |
- Shipped within 5-10 working days.
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Methylated Lysine Antibody (APC) |
abx448362-100ug | Abbexa | 100 ug | EUR 592 |
- Shipped within 5-12 working days.
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Methylated Lysine Antibody (FITC) |
abx448376-100ug | Abbexa | 100 ug | EUR 578 |
- Shipped within 5-12 working days.
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Methylated Lysine Antibody (PerCP) |
abx448396-100ug | Abbexa | 100 ug | EUR 592 |
- Shipped within 5-12 working days.
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Methylated Lysine Antibody (RPE) |
abx448404-100ug | Abbexa | 100 ug | EUR 592 |
- Shipped within 5-12 working days.
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Methylated Lysine Antibody (Streptavidin) |
abx448412-100ug | Abbexa | 100 ug | EUR 592 |
- Shipped within 5-12 working days.
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Methylated Lysine Antibody (Biotin) |
abx448446-400ul | Abbexa | 400 ul | EUR 578 |
- Shipped within 5-12 working days.
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