PRINCIPLE & PROCEDURE
The EpiQuik Circulating Full Histone H3 Quantification Bundle (Colorimetric) is designed for measuring entire histone H3 in plasma or serum. In an assay with this tools, the histone H3 proteins throughout the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.
What’s DNA Methylation?
DNA methylation occurs by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island throughout the promoter space of a gene is methylated, expression of the gene is repressed. Plenty of human diseases akin to most cancers occur when DNA methylation is not going to be appropriately established and/or maintained.
DNA methylation is a dynamic and reversible course of that may result in a variety of intermediate modification varieties. 5-mC remodeled from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is further modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the equivalent TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.
Which DNA Methylation System is Correct for Your Experiment?
There are a variety of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important considerations, selecting the right approach is most important for the success of the analysis.
Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If completely different researchers have already completed the legwork, you might as properly reap the advantages of this accessible information as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely completely different and attention-grabbing, reviewing the beforehand completed work fastidiously to find out potential obstacles or points that the sooner researchers encountered.
epigentek
In the event you’re undecided which approach will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you can assess whether or not or not your sample accommodates 5-mC or 5-hmC and the best way so much. Then you can resolve the following step and which approach is appropriate to utilize. 2) For many who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing could very properly be used.
This method can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing may meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or a variety of genomic areas, targeted bisulfite-sequencing or targeted oxidative bisulfite-sequencing will work.
Methylated DNA Quantification
DNA methylation performs a mandatory operate in common organismal enchancment and cellular differentiation in bigger organisms. Gene expression and the occasion of just about all styles of most cancers are moreover tied to DNA methylation. For example, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency because of a variety of environmental influences and has been proposed as a molecular marker in a variety of natural processes akin to most cancers. World quantification of DNA methylation is important for understanding the roles that gene expression and silencing play in most cancers enchancment and completely different diseases.
 BOK siRNA |
| 20-abx909215 | Abbexa | | |
- Shipped within 5-10 working days.
|
BOK siRNA |
| 20-abx909216 | Abbexa | | |
- Shipped within 5-10 working days.
|
 BOK Antibody |
| 1-CSB-PA892353LA01HU | Cusabio | | |
- Form: Liquid
- Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
|
Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
BOK Antibody |
| 1-CSB-PA904266 | Cusabio | | |
- Form: Liquid
- Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
|
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100 |
 Bok antibody |
| 70R-11577 | Fitzgerald | 100 ug | EUR 536.4 |
Description: Rabbit polyclonal Bok antibody |
 BOK Peptide |
| 42-325P | ProSci | 0.1 mg | EUR 405.6 |
Description: (IN) BOK Peptide |
BOK Antibody |
| 24253-100ul | SAB | 100ul | EUR 468 |
BOK Antibody |
| 3357-002mg | ProSci | 0.02 mg | EUR 206.18 |
- Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
|
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation. |
BOK Antibody |
| 3357-01mg | ProSci | 0.1 mg | EUR 523.7 |
- Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
|
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation. |
BOK Peptide |
| 3357P | ProSci | 0.05 mg | EUR 197.7 |
Description: (NT) BOK peptide |
Bok antibody |
| 20R-1439 | Fitzgerald | 100 ug | EUR 781.2 |
Description: Rabbit polyclonal Bok antibody |
BOK siRNA |
| 20-abx900655 | Abbexa | | |
- Shipped within 5-10 working days.
|
 Bok Antibody |
| 3043-100 | Biovision | each | EUR 430.8 |
