PRINCIPLE & PROCEDURE

The EpiQuik  Circulating Full Histone H3 Quantification Bundle (Colorimetric) is designed for measuring entire histone H3 in plasma or serum. In an assay with this tools, the histone H3 proteins throughout the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.

What’s DNA Methylation?

DNA methylation occurs by the covalent addition of a methyl group (CH³) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island throughout the promoter space of a gene is methylated, expression of the gene is repressed. Plenty of human diseases akin to most cancers occur when DNA methylation is not going to be appropriately established and/or maintained.

DNA methylation is a dynamic and reversible course of that may result in a variety of intermediate modification varieties. 5-mC remodeled from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is further modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the equivalent TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.

Which DNA Methylation System is Correct for Your Experiment?

There are a variety of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important considerations, selecting the right approach is most important for the success of the analysis.

Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If completely different researchers have already completed the legwork, you might as properly reap the advantages of this accessible information as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely completely different and attention-grabbing, reviewing the beforehand completed work fastidiously to find out potential obstacles or points that the sooner researchers encountered.

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In the event you’re undecided which approach will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you can assess whether or not or not your sample accommodates 5-mC or 5-hmC and the best way so much. Then you can resolve the following step and which approach is appropriate to utilize. 2) For many who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing could very properly be used.

This method can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing may meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or a variety of genomic areas, targeted bisulfite-sequencing or targeted oxidative bisulfite-sequencing will work.

Methylated DNA Quantification

DNA methylation performs a mandatory operate in common organismal enchancment and cellular differentiation in bigger organisms. Gene expression and the occasion of just about all styles of most cancers are moreover tied to DNA methylation. For example, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency because of a variety of environmental influences and has been proposed as a molecular marker in a variety of natural processes akin to most cancers. World quantification of DNA methylation is important for understanding the roles that gene expression and silencing play in most cancers enchancment and completely different diseases.

Bok antibody
20R-1439	Fitzgerald	100 ug	EUR 651
Description: Rabbit polyclonal Bok antibody

Bok antibody
70R-11577	Fitzgerald	100 ug	EUR 447
Description: Rabbit polyclonal Bok antibody

BOK Antibody
1-CSB-PA892353LA01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

BOK Antibody
1-CSB-PA904266	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Form: Liquid Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

BOK Antibody
DF3829	Affbiotech	200ul	EUR 304
Description: BOK Antibody detects endogenous levels of total BOK.

BOK Antibody
1-CSB-PA437965	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Form: Liquid Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:20-1:100

BOK siRNA
20-abx900655	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

BOK siRNA
20-abx909215	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

BOK siRNA
20-abx909216	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

BOK Antibody
ABD3829	Lifescience Market	100 ug	EUR 438

HEK-293T Telomerase Over-Expressing Cell Pellet
abx069991-1Pellet	Abbexa	1 Pellet	EUR 398
Shipped within 1-3 working days.

BOK Polyclonal Antibody
29816-100ul	SAB	100ul	EUR 252

BOK Polyclonal Antibody
29816-50ul	SAB	50ul	EUR 187

BOK Rabbit pAb
A16354-100ul	Abclonal	100 ul	EUR 308

BOK Rabbit pAb
A16354-200ul	Abclonal	200 ul	EUR 459

BOK Rabbit pAb
A16354-20ul	Abclonal	20 ul	EUR 183

BOK Rabbit pAb
A16354-50ul	Abclonal	50 ul	EUR 223

Polyclonal Bok Antibody
APR00010G	Leading Biology	0.1mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Bok . This antibody is tested and proven to work in the following applications:

BOK Blocking Peptide
DF3829-BP	Affbiotech	1mg	EUR 195

Polyclonal BOK Antibody
APR06350G	Leading Biology	0.1 mg	EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BOK . This antibody is tested and proven to work in the following applications:

BOK Polyclonal Antibody
ABP57917-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90 Applications tips:
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90