PRINCIPLE & PROCEDURE
The EpiQuik Circulating Full Histone H3 Quantification Bundle (Colorimetric) is designed for measuring entire histone H3 in plasma or serum. In an assay with this tools, the histone H3 proteins throughout the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.
What’s DNA Methylation?
DNA methylation occurs by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island throughout the promoter space of a gene is methylated, expression of the gene is repressed. Plenty of human diseases akin to most cancers occur when DNA methylation is not going to be appropriately established and/or maintained.
DNA methylation is a dynamic and reversible course of that may result in a variety of intermediate modification varieties. 5-mC remodeled from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is further modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the equivalent TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.
Which DNA Methylation System is Correct for Your Experiment?
There are a variety of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important considerations, selecting the right approach is most important for the success of the analysis.
Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If completely different researchers have already completed the legwork, you might as properly reap the advantages of this accessible information as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely completely different and attention-grabbing, reviewing the beforehand completed work fastidiously to find out potential obstacles or points that the sooner researchers encountered.
epigentek
In the event you’re undecided which approach will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you can assess whether or not or not your sample accommodates 5-mC or 5-hmC and the best way so much. Then you can resolve the following step and which approach is appropriate to utilize. 2) For many who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing could very properly be used.
This method can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing may meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or a variety of genomic areas, targeted bisulfite-sequencing or targeted oxidative bisulfite-sequencing will work.
Methylated DNA Quantification
DNA methylation performs a mandatory operate in common organismal enchancment and cellular differentiation in bigger organisms. Gene expression and the occasion of just about all styles of most cancers are moreover tied to DNA methylation. For example, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency because of a variety of environmental influences and has been proposed as a molecular marker in a variety of natural processes akin to most cancers. World quantification of DNA methylation is important for understanding the roles that gene expression and silencing play in most cancers enchancment and completely different diseases.
 BOK siRNA | |||
| 20-abx909215 | Abbexa |
|
|
| |||
BOK siRNA | |||
| 20-abx909216 | Abbexa |
|
|
| |||
 BOK Antibody | |||
| 1-CSB-PA437965 | Cusabio |
|
|
| |||
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:20-1:100 | |||
BOK Antibody | |||
| 1-CSB-PA904266 | Cusabio |
|
|
| |||
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100 | |||
BOK Antibody | |||
| 1-CSB-PA892353LA01HU | Cusabio |
|
|
| |||
Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 | |||
BOK Antibody | |||
| DF3829 | Affbiotech | 200ul | EUR 304 |
Description: BOK Antibody detects endogenous levels of total BOK. | |||
BOK Antibody | |||
| ABD3829 | Lifescience Market | 100 ug | EUR 438 |
 Bok Antibody | |||
| 3043-100 | Biovision | EUR 359 | |
 HEK-293T Telomerase Over-Expressing Cell Pellet | |||
| abx069991-1Pellet | Abbexa | 1 Pellet | EUR 398 |
| |||
 BOK Polyclonal Antibody | |||
| ES9443-100ul | ELK Biotech | 100ul | EUR 279 |
Description: A Rabbit Polyclonal antibody against BOK from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA | |||
BOK Polyclonal Antibody | |||
| ES9443-50ul | ELK Biotech | 50ul | EUR 207 |
Description: A Rabbit Polyclonal antibody against BOK from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA | |||
 BOK Rabbit pAb | |||
| A16354-100ul | Abclonal | 100 ul | EUR 308 |
BOK Rabbit pAb | |||
| A16354-200ul | Abclonal | 200 ul | EUR 459 |
BOK Rabbit pAb | |||
| A16354-20ul | Abclonal | 20 ul | EUR 183 |
BOK Rabbit pAb | |||
| A16354-50ul | Abclonal | 50 ul | EUR 223 |
BOK Polyclonal Antibody | |||
| ABP57917-003ml | Abbkine | 0.03ml | EUR 158 |
| |||
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90 | |||
BOK Polyclonal Antibody | |||
| ABP57917-01ml | Abbkine | 0.1ml | EUR 289 |
| |||
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90 | |||
BOK Polyclonal Antibody | |||
| ABP57917-02ml | Abbkine | 0.2ml | EUR 414 |
| |||
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90 | |||
 Anti-BOK antibody | |||
| STJ72368 | St John's Laboratory | 100 µg | EUR 359 |
 Polyclonal BOK Antibody | |||
| APR06350G | Leading Biology | 0.1 mg | EUR 659 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BOK . This antibody is tested and proven to work in the following applications: | |||
Anti-BOK antibody | |||
| STJ190601 | St John's Laboratory | 200 µl | EUR 197 |
Description: Unconjugated Rabbit polyclonal to BOK | |||
