Bioassay Know-how Laboratory

bioassay

This serological assay may be utilized for functions like characterizing immune responses to viral an an infection by determining the presence of viral antigen-specific antibodies in contaminated and recovered affected particular person sera. At Progressive Evaluation, we provide reliable, fixed merchandise that ship reliable, fixed outcomes.

Molecular Enhancements determine, SARS-CoV-2 IgG Seroconversion ELISA Tools and product amount, SARSCOV2GKT.

This SARS-CoV-2 IgG Seroconversion ELISA Tools from Progressive Evaluation is supposed for the qualitative willpower of SARS-CoV-2 specific antibodies of IgG class in plasma or serum samples. Strip successfully format. Reagents for as a lot as 96 assessments. For evaluation solely. 

This assay has been notably developed using the SARS-CoV-2 receptor binding space (RBD) of the spike protein. This delivers reliable and fixed outcomes with a extreme diploma of reproducibility.Â

This assay could also be carried out in 70 minutes, and has an basic sensitivity of 97-100% and specificity of 100%.

Principle of the Assay:

SARS-CoV-2 specific antibodies will bind to the purified recombinant HEK cell derived receptor-binding space (RBD) of the SARS-CoV-2 spike protein coated on the microtiter plate. After acceptable washing steps, horseradish peroxidase labeled polyclonal anti-human IgG secondary antibody binds to the captured protein. Further secondary antibody is washed away and TMB substrate is used for shade progress at 450 nm. Samples that exceed a determined cut-off price are designated optimistic by this assay.

Further Particulars:

  • Detection Aim: SARS-CoV-2 specific antibodies of isotype IgG
  • Dilution of Samples: A 1:51 dilution of serum samples is normally beneficial for best outcomes.
  • Anticipated Values: Important seroconversion as demonstrated by antibodies specific to every the full-length SARS-CoV-2 spike protein and the RBD space has been detected as early as two days put up symptom onset.
  • Sensitivity: 97% for samples obtained larger than 14 days after symptom onset (n=37), 100% for samples obtained larger than 21 days after symptom onset (n=32).
  • Specificity: 100% (n=89)
  • P.c Settlement: Positive 100%; Damaging 91%
  • Storage: Retailer all bundle elements at 4°C upon arrival. Return any unused microplate strips to the plate pouch with desiccant. Reconstituted controls may be saved at -80°C for later use. Retailer all totally different unused bundle elements at 4°C. This bundle should not be used previous the expiration date.

Damaging outcomes do not preclude acute SARS-CoV-2 an an infection. If acute an an infection is suspected, direct testing for SARS-CoV-2 is important. Outcomes from antibody testing should not be used to diagnose or exclude acute SARS-CoV-2 an an infection. Constructive outcomes may be as a consequence of earlier or present an an infection with non-SARS-CoV-2 coronavirus strains, comparable to KHU1, NL63, OC43, or 229E.

bioassay

bioassay

 

About Bioassay Know-how Laboratory

BT Lab current high-quality and cheap ELISA devices for lab researchers. They’ve a rich pipeline of 6,000+ ELISA kits along with Sandwich ELISA kits and Aggressive EIA (Enzyme Immunoassay) kits.

Product Disclaimer

This supplies is purchased for in-vitro use only for manufacturing and evaluation. This supplies is not applicable for human or animal use. Whereas we make every effort to ensure the safety of our merchandise, we advise coping with any natural provides with customary precautions as if capable of spreading infectious sickness. The statements herin are equipped for informational capabilities solely to be used solely in your consideration, investigation, and verification.

Please keep in mind the image pictured is for illustrative capabilities solely, and your product packaging and look might vary.

Nuclear/Cytosol Fractionation Kit

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Mitochondria/Cytosol Fractionation Kit

K2103-100 100 assays
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Nuclear/Cytosol Fractionation Kit

K266-25
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Mitochondria/Cytosol Fractionation Kit

K256-25
EUR 240

Nuclear/Cytosol Fractionation Kit

K2111-25 25 assays
EUR 251

Mitochondria/Cytosol Fractionation Kit

K2103-25 25 assays
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MCB Glutathione Detection Kit (100 assays)

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Acetylcholinesterase Activity Assay Kit - 100 Assays

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AccuGreen High Sensitivity dsDNA Quantitation Kit (100 assays)

31066-T 100kit
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Description: Minimum order quantity: 1 unit of 100kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Kit (100 assays)

31069-T 1kit
EUR 124
Description: Minimum order quantity: 1 unit of 1kit

Mitochondria Fractionation Kit

55R-1306 25 assays
EUR 296
Description: Mitochondria Fractionation Kit for use in the research laboratory

Nuclear Fractionation Kit

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Description: Nuclear Fractionation Kit for use in the research laboratory

Annexin V, R-phycoerythrin conjugate (100 assays)

9-29045
  • EUR 149.00
  • EUR 312.00
  • 100uL
  • 500uL
Description: Minimum order quantity: 1 unit of 500uL

Annexin V, Allophycocyanin crosslinked conjugate (100 assays)

9-29057
  • EUR 149.00
  • EUR 312.00
  • 100uL
  • 500uL
Description: Minimum order quantity: 1 unit of 500uL

JC-1 Mitochondrial Membrane Potential Detection Kit (100 assays)

30001 1KIT
EUR 301
Description: Minimum order quantity: 1 unit of 1KIT

AccuGreen High Sensitivity dsDNA Quantitation Solution (100 assays)

31068-T 100kit
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Description: Minimum order quantity: 1 unit of 100kit

AccuGreenâ„¢ Broad Range dsDNA Quantitation Solution (100 assays)

31070-T 1kit
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Description: Minimum order quantity: 1 unit of 1kit

Nuclear/Cytosolic Fractionation Kit

AKR-171 20 preps
EUR 276
Description: Cell Biolabs? Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells.  The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours.  The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.

Nuclear/Cytosolic Fractionation Kit

AKR-172 100 preps
EUR 664
Description: Cell Biolabs? Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells.  The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours.  The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.

FractionPREP? Cell Fractionation Kit

K270-50
EUR 550

FractionPREP? Cell Fractionation Kit

K2115-50 50 assays
EUR 599

NucView 488 and MitoView 633 Apoptosis Assay Kit (100 assays)

30062 1KIT
EUR 429
Description: Minimum order quantity: 1 unit of 1KIT

NucView 488 and RedDot 2 Apoptosis & Necrosis Kit (100 assays)

30072 1KIT
EUR 382
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30008-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

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Description: Minimum order quantity: 1 unit of 1KIT

Cytosol/Particulate Separation Kit

55R-1356 50 assays
EUR 551
Description: Cytosol/Particulate Separation Kit for use in the research laboratory

Cytosol/Particulate Separation Kit

K267-50
EUR 403

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ViaFluor 488 SE Cell Proliferation Kit, Trial Size (1 vial, 100 assays)

30086-T 1KIT
EUR 104
Description: Minimum order quantity: 1 unit of 1KIT

LB-100

B4846-100 100 mg
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AIM-100

A3148-100 100 mg
EUR 1859
Description: AIM-100 is a small inhibitor of Ack1 tyrosine kinase with IC50 value of 24 nM [1]. AIM-100 mimicked ATP and inhibited the activity of Ack1 significantly and specifically.

FractionPREP Cell Fractionation system

55R-1359 50 assays
EUR 758
Description: Cell Fractionation system for use in the research laboratory

ReadiPrep™ Nuclear/Cytoplasmic Fractionation Kit

60000 50 Tests
EUR 306
  • R-phrase: R20, R21, R22
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  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

ReadiPrep™ Mitochondrial/Cytoplasmic Fractionation Kit

60005 50 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

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Etoposide (100 mM)

1043-100
EUR 153

S-100 Antibody

3958-100
EUR 316

 

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