This serological assay may be utilized for functions like characterizing immune responses to viral an an infection by determining the presence of viral antigen-specific antibodies in contaminated and recovered affected particular person sera. At Progressive Evaluation, we provide reliable, fixed merchandise that ship reliable, fixed outcomes.
Molecular Enhancements determine, SARS-CoV-2 IgG Seroconversion ELISA Tools and product amount, SARSCOV2GKT.
This SARS-CoV-2 IgG Seroconversion ELISA Tools from Progressive Evaluation is supposed for the qualitative willpower of SARS-CoV-2 specific antibodies of IgG class in plasma or serum samples. Strip successfully format. Reagents for as a lot as 96 assessments. For evaluation solely.Â
This assay has been notably developed using the SARS-CoV-2 receptor binding space (RBD) of the spike protein. This delivers reliable and fixed outcomes with a extreme diploma of reproducibility.Â
This assay could also be carried out in 70 minutes, and has an basic sensitivity of 97-100% and specificity of 100%.
Principle of the Assay:
SARS-CoV-2 specific antibodies will bind to the purified recombinant HEK cell derived receptor-binding space (RBD) of the SARS-CoV-2 spike protein coated on the microtiter plate. After acceptable washing steps, horseradish peroxidase labeled polyclonal anti-human IgG secondary antibody binds to the captured protein. Further secondary antibody is washed away and TMB substrate is used for shade progress at 450 nm. Samples that exceed a determined cut-off price are designated optimistic by this assay.
Further Particulars:
- Detection Aim: SARS-CoV-2 specific antibodies of isotype IgG
- Dilution of Samples: A 1:51 dilution of serum samples is normally beneficial for best outcomes.
- Anticipated Values: Important seroconversion as demonstrated by antibodies specific to every the full-length SARS-CoV-2 spike protein and the RBD space has been detected as early as two days put up symptom onset.
- Sensitivity:Â 97% for samples obtained larger than 14 days after symptom onset (n=37), 100% for samples obtained larger than 21 days after symptom onset (n=32).
- Specificity:Â 100% (n=89)
- P.c Settlement: Positive 100%; Damaging 91%
- Storage: Retailer all bundle elements at 4°C upon arrival. Return any unused microplate strips to the plate pouch with desiccant. Reconstituted controls may be saved at -80°C for later use. Retailer all totally different unused bundle elements at 4°C. This bundle should not be used previous the expiration date.
Damaging outcomes do not preclude acute SARS-CoV-2 an an infection. If acute an an infection is suspected, direct testing for SARS-CoV-2 is important. Outcomes from antibody testing should not be used to diagnose or exclude acute SARS-CoV-2 an an infection. Constructive outcomes may be as a consequence of earlier or present an an infection with non-SARS-CoV-2 coronavirus strains, comparable to KHU1, NL63, OC43, or 229E.
bioassay
About Bioassay Know-how Laboratory
BT Lab current high-quality and cheap ELISA devices for lab researchers. They’ve a rich pipeline of 6,000+ ELISA kits along with Sandwich ELISA kits and Aggressive EIA (Enzyme Immunoassay) kits.
Product Disclaimer
This supplies is purchased for in-vitro use only for manufacturing and evaluation. This supplies is not applicable for human or animal use. Whereas we make every effort to ensure the safety of our merchandise, we advise coping with any natural provides with customary precautions as if capable of spreading infectious sickness. The statements herin are equipped for informational capabilities solely to be used solely in your consideration, investigation, and verification.
Please keep in mind the image pictured is for illustrative capabilities solely, and your product packaging and look might vary.
Nuclear/Cytosol Fractionation Kit |
K2111-100 | ApexBio | 100 assays | EUR 652.8 |
Nuclear/Cytosol Fractionation Kit |
K2111-25 | ApexBio | 25 assays | EUR 301.2 |
Mitochondria/Cytosol Fractionation Kit |
K256-100 | Biovision | each | EUR 614.4 |
Mitochondria/Cytosol Fractionation Kit |
K256-25 | Biovision | each | EUR 288 |
Mitochondria/Cytosol Fractionation Kit |
K2103-100 | ApexBio | 100 assays | EUR 634.8 |
Mitochondria/Cytosol Fractionation Kit |
K2103-25 | ApexBio | 25 assays | EUR 301.2 |
Nuclear/Cytosolic Fractionation Kit |
AKR-171 | Cell Biolabs | 20 preps | EUR 331.2 |
Description: Cell Biolabs? Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting. |
Nuclear/Cytosolic Fractionation Kit |
AKR-172 | Cell Biolabs | 100 preps | EUR 796.8 |
Description: Cell Biolabs? Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting. |
Nuclear Fractionation Kit |
55R-1307 | Fitzgerald | 25 assays | EUR 393.6 |
Description: Nuclear Fractionation Kit for use in the research laboratory |
FractionPREP? Cell Fractionation Kit |
K270-50 | Biovision | each | EUR 660 |
FractionPREP? Cell Fractionation Kit |
K2115-50 | ApexBio | 50 assays | EUR 718.8 |
Mitochondria Fractionation Kit |
55R-1306 | Fitzgerald | 25 assays | EUR 355.2 |
Description: Mitochondria Fractionation Kit for use in the research laboratory |
ReadiPrepâ„¢ Nuclear/Cytoplasmic Fractionation Kit |
60000-50Tests | AAT Bioquest | 50 Tests | EUR 334 |
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Description: The ReadiPrep™ Nuclear/Cytoplasmic Fractionation Kit offers stepwise isolation of nuclear and cytoplasmic extract from mammalian cells or tissue. |
ReadiPrep™ Nuclear/Cytoplasmic Fractionation Kit |
60000 | AAT Bioquest | 50 Tests | EUR 334 |
ReadiPrepâ„¢ Mitochondrial/Cytoplasmic Fractionation Kit |
60005-50Tests | AAT Bioquest | 50 Tests | EUR 334 |
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Description: The ReadiPrepâ„¢ Mitochondrial/Cytoplasmic Fractionation Kit offers a convenient method for isolating mitochondria and cytoplasmic fractions from mammalian cells or tissue. |
ReadiPrep™ Mitochondrial/Cytoplasmic Fractionation Kit |
60005 | AAT Bioquest | 50 Tests | EUR 334 |
FractionPREP Cell Fractionation system |
55R-1359 | Fitzgerald | 50 assays | EUR 909.6 |
Description: Cell Fractionation system for use in the research laboratory |
Adipose Protein Fractionation Kit (water-soluble/insoluble) |
P523-2 | 101Bio | NULL | Ask for price |
Adipose Protein Fractionation Kit (water-soluble/insoluble) |
P523-20 | 101Bio | NULL | Ask for price |
Adipose Protein Fractionation Kit (water-soluble/insoluble) |
P523-80 | 101Bio | NULL | Ask for price |
Goat Anti-Mouse IgG, Salt Fractionation (Bulk) |
41319-GAF-BLK | Alpha Diagnostics | 1g; bulk | Ask for price |
Goat Anti-Bovine IgG, Salt Fractionation (Bulk) |
60319-CAF-BLK | Alpha Diagnostics | 1g; bulk | Ask for price |
Goat Anti-Bovine IgG, Salt Fractionation (Bulk) |
60319-GAF-BLK | Alpha Diagnostics | 1g; bulk | Ask for price |
Donkey Anti-Sheep IgG, Salt Fractionation (Bulk) |
30326-CAS-BLK | Alpha Diagnostics | 1g; bulk | Ask for price |
Fractionation Purified Rabbit Anti-Mouse Albumin Polyclonal Antibody |
rAlbAb-FP | Polysciences Europe GmbH | NULL | EUR 355 |
Description: Antibodies |
Goat IgG Fractionated Purified |
IGTIGGGF10GM | Innovative research | each | EUR 1058 |
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Description: Goat IgG Fractionated Purified |
Goat IgG Fractionated Purified |
IGTIGGGF1GM | Innovative research | each | EUR 211 |
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Description: Goat IgG Fractionated Purified |
Human IgG Fractionated Purified |
IHUIGGGF10GM | Innovative research | each | EUR 2240 |
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Description: Human IgG Fractionated Purified |
Human IgG Fractionated Purified |
IHUIGGGF1GM | Innovative research | each | EUR 269 |
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Description: Human IgG Fractionated Purified |
Horse IgG Fractionated Purified |
IHSIGGGF10GM | Innovative research | each | EUR 1264 |
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Description: Horse IgG Fractionated Purified |
Horse IgG Fractionated Purified |
IHSIGGGF1GM | Innovative research | each | EUR 464 |
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Description: Horse IgG Fractionated Purified |
Mouse IgG Fractionated Purified |
IMSIGGGF100MG | Innovative research | each | EUR 360 |
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Description: Mouse IgG Fractionated Purified |
Mouse IgG Fractionated Purified |
IMSIGGGF1GM | Innovative research | each | EUR 1104 |
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Description: Mouse IgG Fractionated Purified |
Mouse IgG Fractionated Purified |
IMSIGGGF500MG | Innovative research | each | EUR 592 |
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Description: Mouse IgG Fractionated Purified |
Rabbit IgG Fractionated Purified |
IRBIGGGF100GM | Innovative research | each | EUR 23917 |
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Description: Rabbit IgG Fractionated Purified |