|Concentrated HIV-1 p24 Antigen Standard (0.3mL), SIGMAFAST™ OPD tablet sets (5 sets)|
|Store at 2° to 8°C|
|Sufficient for 5 x 96 determinations|
PRINCIPLE OF THE TEST
Many specimens and tissue culture samples contain HIV-1 viral loads greater than the range of the standard curve of commercially available antigen ELISA’s. In order to quantify such samples, a series of expensive and time consuming dilutions must be made to adjust the sample concentration. The RETRO-TEK HIV-1 p24 Extended Range Kit includes reagents that extend the standard curve of the up to 4 ng/mL for either the HIV-1 p24 ELISA (catalog #: 0801111 or 0801200) or the HIV-1 p24 Antigen ELISA 2.0 (catalog #: 0801002 or 0801008). Therefore, samples containing p24 antigen up to 4 ng/mL may be accurately quantified without extensive serial dilution.
• Concentrated HIV-1 p24 Antigen Standard (0.3mL): Contains detergent disrupted, heat inactivated viral antigen at a concentration of 80 ng/mL, added protein, detergent and 2-chloroacetamide.
• Substrate (5 sets): SIGMAFASTTM OPD tablet sets.
Contains: 5 OPD tablets (o-phenylenediamine dihydrochloride) [silver foil]
Contains: 5 urea-hydrogen peroxide tablets [gold foil]
SIGMAFASTTM is a registered trademark of the Sigma-Aldrich Company.
Storage: Store all kit reagents at 2°-8°C. Materials Required but Not Supplied
• HIV-1 p24 Antigen ELISA (catalog #: 0801111 or 0801200) or
HIV-1 p24 Antigen ELISA 2.0 (catalog #: 0801002 or 0801008)
• Distilled or deionized water
• Test tubes and racks for preparing specimen and standard dilutions
• Adjustable micropipettes, single and multichannel
• Incubator capable of maintaining 37º± 1º C
• Graduated cylinders and assorted beakers
• Automatic microplate washer or manual vacuum aspiration equipment
• 1% sodium hypochlorite as disinfectant. May be prepared from household bleach
• OPD is a possible carcinogen. Avoid contact. Use gloves when handling tablets.
• Use Universal Precautions when handling test specimens and HIV-1 p24 Antigen Standard.*
• Disposal: When examining human source material or other potentially infectious specimens, adhere to all applicable local, state and federal regulations regarding disposal of hazardous materials.
• To avoid cross-contamination, use separate pipet tips for each specimen.
* from MMWR, June 24,1988, Vol. 37, No.24, pp. 377-382, 387-8
Allow all reagents to reach room temperature before use. Proceed with the RETRO-TEK HIV-1 p24 ELISA according to the product insert with the following changes:
1. Replace the HIV-1 p24 Antigen Standard supplied with the p24 ELISA kit with the HIV-1 p24 Antigen Extended Range Standard.
2. Substitute the TMB Substrate supplied with the p24 ELISA kit with the OPD Substrate Solution and incubate microplate for 30 minutes at room temperature.
Please Note: Due to plate reader differences, the high standard absorbance values may be out of range. Read plate at 435nm or 405nm if necessary.
CALCULATION AND INTERPRETATION OF RESULTS
Calculate the mean absorbance for each p24 standard and test sample. Plot the concentration of HIV-1 p24 Antigen Standard (pg/mL) on the X-axis versus the mean optical density (OD) for each standard on the Y-axis. The HIV-1 p24 concentration of unknown samples is best determined by using a 4-parameter logistic regression. Alternatively, a point-to-point algorithm may be used. Only readings which fall within the range of the standard curve may be quantified. Be sure to correct for all dilutions, including the 1.1 dilution made during the addition of Lysing Buffer.
Below is an example of a typical standard curve for the RETRO-TEK HIV-1 p24 Antigen ELISA (ZEPTOMETRIX catalog # 0801111 or 0801200).
|SmartCheck™ Tri-color Extended Range Plus Protein Marker, 13 bands 9-245 kDA, 2x250ul|
|PR5013-TC||Benchmark Scientific||1 each|| |
|EMPTY RACK FOR AXYGEN MULTIRACK 200UL EXTENDED LENGTH, 300UL EXTENDED LENTGH, AND 1000 UL EXTENDED LENGTH|
Description: Non Filter Tips; Multi Rack Tips
|Extended Warranty 50|
|EW-50||Next Advance||1each|| |
Description: Extended warranty on a BB50-DX Bullet Blender, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.
|Extended Warranty BBX|
|EW-BBX||Next Advance||1each|| |
Description: Extended warranty on a BBX series Bullet Blender, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.
|Extended Warranty BBY|
|EW-BBY||Next Advance||1each|| |
Description: Extended warranty on a BBY series Bullet Blender, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.
|Extended Warranty GOLD|
|EW-GOLD||Next Advance||1each|| |
Description: Extended warranty on a GOLD series Bullet Blender, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.
|Extended Warranty BlotBot|
|EW-FRB||Next Advance||1each|| |
Description: Extended warranty on a Freedom Rocker BlotBot, from 2 to 4 years. Includes priority on repairs, parts, labor and domestic shipping.
|Prestige Autoclave 2100 Extended|
|AUT4502||Scientific Laboratory Supplies||EACH|| |
|Prestige Autoclave 2100 Extended+|
|AUT4504||Scientific Laboratory Supplies||EACH|| |
|Axygen Empty rack for MultiRack 200uL extended length 300uL and 1000 uL extended length|
|PIP1328||Scientific Laboratory Supplies||PK5|| |
|Extended Fine Tip Mini Pastette|
|127-P423-000||Scientific Laboratory Supplies||PK400|| |
|Rat Extended Synaptotagmin 1 (ESYT1) ELISA Kit|
|abx391308-96tests||Abbexa||96 tests|| |
|Kinesis Extended Nut 1.2mm Hole; SS|
|CHR2091||Scientific Laboratory Supplies||PK5|| |
|Arium Extended Connection Set Pure|
|WAT4348||Scientific Laboratory Supplies||EACH|| |
|Mouse Extended Synaptotagmin 2 (ESYT2) ELISA Kit|
|abx389231-96tests||Abbexa||96 tests|| |
|Mouse Extended Synaptotagmin 1 (ESYT1) ELISA Kit|
|abx389232-96tests||Abbexa||96 tests|| |
|Mouse Extended Synaptotagmin 3 (ESYT3) ELISA Kit|
|abx389233-96tests||Abbexa||96 tests|| |
|Human Extended Synaptotagmin 2 (ESYT2) ELISA Kit|
|abx384873-96tests||Abbexa||96 tests|| |
|Human Extended Synaptotagmin 3 (ESYT3) ELISA Kit|
|abx384874-96tests||Abbexa||96 tests|| |
|Mouse Extended synaptotagmin- 1, Esyt1 ELISA KIT|
|ELI-48047m||Lifescience Market||96 Tests|| |
|Human Extended synaptotagmin- 3, ESYT3 ELISA KIT|
|ELI-48048h||Lifescience Market||96 Tests|| |
|Human Extended synaptotagmin- 2, ESYT2 ELISA KIT|
|ELI-26958h||Lifescience Market||96 Tests|| |
|Mouse Extended synaptotagmin- 2, Esyt2 ELISA KIT|
|ELI-32779m||Lifescience Market||96 Tests|| |
|Human Extended synaptotagmin- 1, ESYT1 ELISA KIT|
|ELI-09959h||Lifescience Market||96 Tests|| |
|Mouse Extended synaptotagmin- 3, Esyt3 ELISA KIT|
|ELI-09960m||Lifescience Market||96 Tests|| |
|Human Extended synaptotagmin-1 (ESYT1) ELISA Kit|
|abx392223-96tests||Abbexa||96 tests|| |
|Kinesis Extended Nut 1/16 Hole; SS|
|CHR2097||Scientific Laboratory Supplies||PK5|| |
|Human Extended synaptotagmin-1 (MBC2)|
Description: Recombinant Human Extended synaptotagmin-1(MBC2),partial expressed in E.coli
|Antibody to Hepatitis C Virus ELISA Kit (Extended)|
|DEIA062||Creative Diagnostics||96T|| |
Description: This kit is an enzyme-linked immunosorbent assay for qualitative detection of antibodies to hepatitis C virus in human serum or plasma. It is intended forscreening blood donors and diagnosing patients related to infection with hepatitis C virus.
|Arium Extended Connection Set Ultrapure|
|WAT4350||Scientific Laboratory Supplies||EACH|| |
|Kinesis Extended Nut 1/16 Hole; Brass|
|CHR2093||Scientific Laboratory Supplies||PK5|| |
|Extended Synaptotagmin 2 (ESYT2) Antibody|