epigentek chromoplasts chromatin

Epigentek Chromoplasts Chromatin DNA interaction With ChIP Extraction Kit

Background Information

Chromatin immunoprecipitaton (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

epigentek chromoplasts chromatin

epigentek chromoplasts chromatin

Product Overview

The ChromaFlash Chromatin Extraction Package deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle could be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your complete course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Helpful and versatile: the bundle is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 106  cells or per 50 mg tissues.

Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.

Principle & Course of
The ChromaFlash™ Plant Chromatin Extraction Package deal accommodates the entire reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

ChromaFlash Plant Chromatin Extraction Kit 

P-2022-050 50 Extractions
EUR 193.6

ChromaFlash Plant Chromatin Extraction Kit 

P-2022
  • EUR 331.44
  • EUR 193.60
  • 50 Extractions
  • 50 Extractions

ExoDNA? Extraction Kit

K1242-25
EUR 705.6

ExoDNA? Extraction Kit

K1242-50
EUR 1057.2

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Membrane Protein Extraction Kit

55R-1357 50 assays
EUR 888
Description: Membrane Protein Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit

55R-1358 500 assays
EUR 457.2
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Quick Gel Extraction Kit

20-abx098085
  • EUR 343.20
  • EUR 243.60
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Up RNA Extraction Kit

20-abx098087
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.

Plant RNA extraction Kit

20-abx098088
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.

Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal

Plant Protein Extraction Kit

BSP004 20Preps
EUR 133.08
  • Product category: Molecular Biology Kits/Protein - Extraction/Plant

Nuclear Protein Extraction Kit

BSP009 50Preps
EUR 106.98
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal

FFPE Protein Extraction Kit

BSP058 50Preps
EUR 122.64
  • Product category: Molecular Biology Kits/Protein - Extraction/FFPE Tissue

Membrane Protein Extraction Kit

K2113-50 50 assays
EUR 652.8

Mammalian Cell Extraction Kit

K2114-500 500 assays
EUR 385.2

Membrane Protein Extraction Kit

K268-50
EUR 646.8

Plant Tissue Extraction Kit

K296-50
EUR 548.4

Total Protein Extraction Kit

K3011010 1 kit
EUR 444
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Mammalian Cell Extraction Kit

K269-500
EUR 352.8

Exosomal RNA Extraction Kit

P200R,-part-of-P200 NULL
EUR 0

Exosomal DNA Extraction Kit

P230-25 NULL
EUR 0

Exosomal DNA Extraction Kit

P230-50 NULL
EUR 0

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

EpiQuik Nuclear Extraction Kit 

OP-0002-1 100 Extractions
EUR 211.2

Membrane Protein Extraction Kit

MPE01-12S 0.5 ml
EUR 117.02
Description: This product includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes.

EpiQuik Nuclear Extraction Kit 

OP-0002
  • EUR 350.58
  • EUR 211.20
  • 100 Assays
  • 100 Extractions

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

EpiQuik Chromatin Accessibility Assay Kit 

P-1047-48 48 Reactions
EUR 611.6

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002-1 24 Reactions
EUR 312.4