Background Information
Chromatin immunoprecipitaton (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

epigentek chromoplasts chromatin
Product Overview
The ChromaFlash Chromatin Extraction Package deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle could be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.
- Terribly fast course of: your complete course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
- Helpful and versatile: the bundle is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
- Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues. Yield of chromatin is roughly 4 ug per 106 cells or per 50 mg tissues.
Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.
Principle & Course of
The ChromaFlash™ Plant Chromatin Extraction Package deal accommodates the entire reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.
ChromaFlash Chromatin Isolation & Shearing Kit |
P-2023 | EpiGentek | | - 100 Preperations
- 100 Preparations
|
miRNA Extraction Kit |
K1456-50 | Biovision | 50 Preps | EUR 661.2 |
ExoDNA? Extraction Kit |
K1242-25 | Biovision | each | EUR 705.6 |
ExoDNA? Extraction Kit |
K1242-50 | Biovision | each | EUR 1057.2 |
EpiQuik Chromatin Accessibility Assay Kit |
P-1047 | EpiGentek | | |
Up RNA Extraction Kit |
20-abx098087 | Abbexa | | |
- Shipped within 5-10 working days.
|
EpiQuik Chromatin Immunoprecipitation (ChIP) Kit |
P-2002 | EpiGentek | - EUR 1084.86
- EUR 312.4
- EUR 500.5
- EUR 738.1
| - 96 Reactions
- 24 Reactions
- 48 Reactions
- 96 Reactions
|
Quick Gel Extraction Kit |
20-abx098085 | Abbexa | | |
- Shipped within 5-10 working days.
|
Plant RNA extraction Kit |
20-abx098088 | Abbexa | | |
- Shipped within 5-10 working days.
|
FFPE Protein Extraction Kit |
BSP058 | Bio Basic | 50Preps | EUR 122.64 |
- Product category: Molecular Biology Kits/Protein - Extraction/FFPE Tissue
|
Plant Tissue Extraction Kit |
K296-50 | Biovision | each | EUR 548.4 |
Exosomal RNA Extraction Kit |
P200R,-part-of-P200 | 101Bio | NULL | Ask for price |
Exosomal DNA Extraction Kit |
P230-25 | 101Bio | NULL | Ask for price |
Exosomal DNA Extraction Kit |
P230-50 | 101Bio | NULL | Ask for price |
Gel Extraction Kit (100 preps) |
9K-006-0001 | Bio Basic | 100preps | EUR 283.42 |
- Product category: Molecular Biology Kits/DNA - Extraction/Gel Extraction
|
Gel Extraction Kit (200 preps) |
9K-006-0002 | Bio Basic | 200preps, 200prep | EUR 418.09 |
- Product category: Molecular Biology Kits/DNA - Extraction/Gel Extraction
|
Total Protein Extraction Kit |
BSP003 | Bio Basic | 50Preps | EUR 130.99 |
- Product category: Molecular Biology Kits/Protein - Extraction/Animal
|
Plant Protein Extraction Kit |
BSP004 | Bio Basic | 20Preps | EUR 133.08 |
- Product category: Molecular Biology Kits/Protein - Extraction/Plant
|
Total Protein Extraction Kit |
K3011010 | Biochain | 1 kit | EUR 367 |
Bacterial RNA Extraction Kit |
R403-01 | Vazyme | 100 rxn | EUR 186 |
Mammalian Cell Extraction Kit |
55R-1358 | Fitzgerald | 500 assays | EUR 457.2 |
Description: Mammalian Cell Extraction Kit for use in the research laboratory |
Mammalian Cell Extraction Kit |
K269-500 | Biovision | each | EUR 352.8 |
Mammalian Cell Extraction Kit |
K2114-500 | ApexBio | 500 assays | EUR 385.2 |
Up Plus RNA Extraction Kit |
20-abx098093 | Abbexa | | |
- Shipped within 5-10 working days.
|
Chromatin protein Cren7 Antibody (HRP) |
20-abx109204 | Abbexa | - EUR 493.2
- EUR 2214
- EUR 718.8
- EUR 218.4
- EUR 360
| - 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
- Shipped within 5-10 working days.
|
Nuclear Protein Extraction Kit |
BSP009 | Bio Basic | 50Preps | EUR 106.98 |
- Product category: Molecular Biology Kits/Protein - Extraction/Animal
|
EpiQuik Nuclear Extraction Kit |
OP-0002 | EpiGentek | | - 100 Assays
- 100 Extractions
|
Triton Extraction Buffer(TEB), for Histon Extraction |
T8300-100 | GenDepot | 100ml | EUR 151.2 |
Triton Extraction Buffer(TEB), for Histon Extraction |
T8300-500 | GenDepot | 500ml | EUR 170.4 |
DNA Extraction Kit in Tubes |
D100T | Cygnus Technologies | 1 kit (50 tubes) | EUR 562.8 |
- Product category: DNA and RNA extraction and purification
|
Description: DNA Extraction Kit in Tubes by Cygnus Technologies is available in Europe via Gentaur. |
DNA Extraction Kit in Wells |
D100W | Cygnus Technologies | 1 kit (96 wells plate) | EUR 562.8 |
- Product category: DNA and RNA extraction and purification
|
Description: DNA Extraction Kit in Wells by Cygnus Technologies is available in Europe via Gentaur. |
Membrane Protein Extraction Kit |
55R-1357 | Fitzgerald | 50 assays | EUR 888 |
Description: Membrane Protein Extraction Kit for use in the research laboratory |