epigentek chromoplasts chromatin

Epigentek Chromoplasts Chromatin DNA interaction With ChIP Extraction Kit

Background Information

Chromatin immunoprecipitaton (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

epigentek chromoplasts chromatin

epigentek chromoplasts chromatin

Product Overview

The ChromaFlash Chromatin Extraction Package deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle could be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your complete course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Helpful and versatile: the bundle is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 106  cells or per 50 mg tissues.

Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.

Principle & Course of
The ChromaFlash™ Plant Chromatin Extraction Package deal accommodates the entire reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023
  • EUR 324.48
  • EUR 189.2
  • 100 Preperations
  • 100 Preparations

EpiQuik Chromatin Accessibility Assay Kit 

P-1047
  • EUR 910.86
  • EUR 611.6
  • 48 Assays
  • 48 Reactions

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

ExoDNA? Extraction Kit

K1242-25 each
EUR 705.6

ExoDNA? Extraction Kit

K1242-50 each
EUR 1057.2

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002
  • EUR 1084.86
  • EUR 312.4
  • EUR 500.5
  • EUR 738.1
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

Up RNA Extraction Kit

20-abx098087
  • EUR 309.6
  • EUR 126
  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.

Chromatin protein Cren7 Antibody (HRP)

20-abx109204
  • EUR 493.2
  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Quick Gel Extraction Kit

20-abx098085
  • EUR 343.2
  • EUR 243.6
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Plant RNA extraction Kit

20-abx098088
  • EUR 309.6
  • EUR 126
  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.

Chromatin protein Cren7 Antibody (FITC)

20-abx107787
  • EUR 493.2
  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Chromatin Modifying Protein 2A Protein

20-abx261989
  • EUR 5388
  • EUR 393.6
  • EUR 276
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Chromatin Modifying Protein 2B Protein

20-abx262025
  • EUR 5388
  • EUR 393.6
  • EUR 276
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Chromatin Modifying Protein 4A Protein

20-abx263203
  • EUR 276
  • EUR 1779.6
  • EUR 393.6
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.

Chromatin Modifying Protein 1A Protein

20-abx263326
  • EUR 1930.8
  • EUR 393.6
  • EUR 276
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.

Chromatin Protein Cren7 (creN7) Antibody

20-abx109437
  • EUR 493.2
  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

ATRX, Chromatin Remodeler (ATRX) Antibody

20-abx325123
  • EUR 376.8
  • EUR 292.8
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

ATRX, Chromatin Remodeler (ATRX) Antibody

abx331300-100ul 100 ul
EUR 510
  • Shipped within 5-10 working days.

Gel Extraction Kit (100 preps)

9K-006-0001 100preps
EUR 283.42
  • Product category: Molecular Biology Kits/DNA - Extraction/Gel Extraction

Gel Extraction Kit (200 preps)

9K-006-0002 200preps, 200prep
EUR 418.09
  • Product category: Molecular Biology Kits/DNA - Extraction/Gel Extraction

FFPE Protein Extraction Kit

BSP058 50Preps
EUR 122.64
  • Product category: Molecular Biology Kits/Protein - Extraction/FFPE Tissue

Exosomal RNA Extraction Kit

P200R,-part-of-P200 NULL Ask for price

Exosomal DNA Extraction Kit

P230-25 NULL Ask for price

Exosomal DNA Extraction Kit

P230-50 NULL Ask for price

Plant Tissue Extraction Kit

K296-50 each
EUR 548.4

Chromatin protein Cren7 Antibody (Biotin)

20-abx106375
  • EUR 493.2
  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Total Protein Extraction Kit

BSP003 50Preps
EUR 130.99
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal

Plant Protein Extraction Kit

BSP004 20Preps
EUR 133.08
  • Product category: Molecular Biology Kits/Protein - Extraction/Plant

Total Protein Extraction Kit

K3011010 1 kit
EUR 367

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 186

Up Plus RNA Extraction Kit

20-abx098093
  • EUR 444
  • EUR 126
  • 100 rxns
  • 2 rxns
  • Shipped within 5-10 working days.

Mammalian Cell Extraction Kit

55R-1358 500 assays
EUR 457.2
Description: Mammalian Cell Extraction Kit for use in the research laboratory