Background Information
Chromatin immunoprecipitaton (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.
epigentek chromoplasts chromatin
Product Overview
The ChromaFlash Chromatin Extraction Package deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle could be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.
- Terribly fast course of: your complete course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
- Helpful and versatile: the bundle is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
- Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues. Yield of chromatin is roughly 4 ug per 106 cells or per 50 mg tissues.
Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.
Principle & Course of
The ChromaFlash™ Plant Chromatin Extraction Package deal accommodates the entire reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.
 ChromaFlash Plant Chromatin Extraction Kit |
| P-2022-050 | EpiGentek | 50 Extractions | EUR 193.6 |
ChromaFlash Plant Chromatin Extraction Kit |
| P-2022 | EpiGentek | | - 50 Extractions
- 50 Extractions
|
 ExoDNA? Extraction Kit |
| K1242-25 | Biovision | | EUR 705.6 |
ExoDNA? Extraction Kit |
| K1242-50 | Biovision | | EUR 1057.2 |
 miRNA Extraction Kit |
| K1456-50 | Biovision | 50 Preps | EUR 661.2 |
 Frit Kit |
| FRIT-KIT | Next Advance | 1each | EUR 148.8 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
 Membrane Protein Extraction Kit |
| 55R-1357 | Fitzgerald | 50 assays | EUR 888 |
Description: Membrane Protein Extraction Kit for use in the research laboratory |
 Mammalian Cell Extraction Kit |
| 55R-1358 | Fitzgerald | 500 assays | EUR 457.2 |
Description: Mammalian Cell Extraction Kit for use in the research laboratory |
 Quick Gel Extraction Kit |
| 20-abx098085 | Abbexa | | |
- Shipped within 5-10 working days.
|
 Up RNA Extraction Kit |
| 20-abx098087 | Abbexa | | |
- Shipped within 5-10 working days.
|
 Plant RNA extraction Kit |
| 20-abx098088 | Abbexa | | |
- Shipped within 5-10 working days.
|
 Total Protein Extraction Kit |
| BSP003 | Bio Basic | 50Preps | EUR 130.99 |
- Product category: Molecular Biology Kits/Protein - Extraction/Animal
|
 Plant Protein Extraction Kit |
| BSP004 | Bio Basic | 20Preps | EUR 133.08 |
- Product category: Molecular Biology Kits/Protein - Extraction/Plant
|
 Nuclear Protein Extraction Kit |
| BSP009 | Bio Basic | 50Preps | EUR 106.98 |
- Product category: Molecular Biology Kits/Protein - Extraction/Animal
|
 FFPE Protein Extraction Kit |
| BSP058 | Bio Basic | 50Preps | EUR 122.64 |
- Product category: Molecular Biology Kits/Protein - Extraction/FFPE Tissue
|
Membrane Protein Extraction Kit |
| K2113-50 | ApexBio | 50 assays | EUR 652.8 |
Mammalian Cell Extraction Kit |
| K2114-500 | ApexBio | 500 assays | EUR 385.2 |
Membrane Protein Extraction Kit |
| K268-50 | Biovision | | EUR 646.8 |
 Plant Tissue Extraction Kit |
| K296-50 | Biovision | | EUR 548.4 |
Total Protein Extraction Kit |
| K3011010 | Biochain | 1 kit | EUR 444 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Mammalian Cell Extraction Kit |
| K269-500 | Biovision | | EUR 352.8 |
 Exosomal RNA Extraction Kit |
| P200R,-part-of-P200 | 101Bio | NULL | EUR 0 |
 Exosomal DNA Extraction Kit |
| P230-25 | 101Bio | NULL | EUR 0 |
Exosomal DNA Extraction Kit |
| P230-50 | 101Bio | NULL | EUR 0 |
 Bacterial RNA Extraction Kit |
| R403-01 | Vazyme | 100 rxn | EUR 186 |
 EpiQuik Nuclear Extraction Kit |
| OP-0002-1 | EpiGentek | 100 Extractions | EUR 211.2 |
Membrane Protein Extraction Kit |
| MPE01-12S | ProFoldin | 0.5 ml | EUR 117.02 |
Description: This product includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes. |
EpiQuik Nuclear Extraction Kit |
| OP-0002 | EpiGentek | | - 100 Assays
- 100 Extractions
|
 Column Packing Kit |
| PACK-KIT | Next Advance | 1pack | EUR 1242 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
 PCR Mycoplasma Detection Kit |
| M034-Kit | TOKU-E | Kit | EUR 319.2 |
 EpiQuik Chromatin Accessibility Assay Kit |
| P-1047-48 | EpiGentek | 48 Reactions | EUR 611.6 |
 Kit ) EpiQuik Chromatin Immunoprecipitation (ChIP) Kit |
| P-2002-1 | EpiGentek | 24 Reactions | EUR 312.4 |
