The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum)).
• Extracts highly pure total RNA (including small RNA fraction) from exosomes
• Recovers protein and RNA from the same sample
• Compatible with all protocols for exosome isolation
The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). It is suitable for studies of RNA expression (specifically miRNA), processing, or function. The kit allows recovery of both RNA and proteins from the same purified exosome preparation. One portion of the sample is subjected to organic extraction, followed by immobilization of RNA on glass-fiber filters to purify total RNA or enrich for small RNA species. The other portion is then used directly for common protein applications such as Western blotting.
High yields of ultra-pure RNA can be prepared in about 30–60 minutes. The RNA can then be used in a variety of downstream applications, including qRT-PCR, high-throughput sequencing (e.g., using the Ion Torent™ PGM™, Ion Torrent Proton, or SOLiD sequencing instruments), microarray analysis, solution hybridization assays, and blot hybridization.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
buffer a exosome protein extraction kit
exoEasy Maxi Kit
For purification of exosomes and other extracellular vesicles (EVs) from plasma, serum and cell culture supernatant
Features
- Isolate intact exosomes and other extracellular vesicles
- Suitable for functional testing and physical and biochemical analysis
- Purify exosomes and other extracellular vesicles in 25 minutes
- Process multiple samples in parallel using a simple spin-column procedure
- Use with up to 4 ml of plasma or serum or 32 ml of cell culture supernatant
- Suitable for MISEV-compliant protein characterization
Product Details
The exoEasy Maxi Kit uses membrane affinity spin columns to efficiently isolate exosomes and other extracellular vesicles from serum, plasma, cell culture supernatant and other biological fluids. The maxi column format allows the use of large sample volumes, including up to 4 ml plasma or serum or up to 32 ml cell culture supernatant. The procedure is very fast, consistent and highly suited for isolation of EVs for functional analysis of exosomes and other extracellular vesicles.
Performance
The exoEasy protocol for purification of exosomes and other extracellular vesicles is not only faster than ultracentrifugation, but also yields a cleaner preparation. Scanning electron microscopy shows that while both techniques deliver intact vesicles of the expected size, the preparation from ultracentrifugation also contains many smaller structures that do not match the expected size or shape for EVs (see figure “ Intact vesicles are eluted from the exoEasy membrane with higher purity compared with ultracentrifugation”).
 Leukemia - ALL Exosome RNA |
| P241-ALL | 101Bio | NULL | EUR 0 |
 Leukemia - AML Exosome RNA |
| P241-AML | 101Bio | NULL | EUR 0 |
 Bladder Cancer Exosome RNA |
| P241-BL | 101Bio | NULL | EUR 0 |
 Breast Cancer Exosome RNA |
| P241-BR | 101Bio | NULL | EUR 0 |
 Leukemia - CLL Exosome RNA |
| P241-CLL | 101Bio | NULL | EUR 0 |
 Leukemia - CML Exosome RNA |
| P241-CML | 101Bio | NULL | EUR 0 |
 Colorectal Cancer Exosome RNA |
| P241-CO | 101Bio | NULL | EUR 0 |
 Crohn's Disease Exosome RNA |
| P241-CR | 101Bio | NULL | EUR 0 |
 Kidney Cancer Exosome RNA |
| P241-KN | 101Bio | NULL | EUR 0 |
 Lung Cancer Exosome RNA |
| P241-LG | 101Bio | NULL | EUR 0 |
 Liver Cancer Exosome RNA |
| P241-LV | 101Bio | NULL | EUR 0 |
 Nasopharyngeal Cancer Exosome RNA |
| P241-NA | 101Bio | NULL | EUR 0 |
 Prostate Cancer Exosome RNA |
| P241-PR | 101Bio | NULL | EUR 0 |
 Rheumatoid Arthritis Exosome RNA |
| P241-RA | 101Bio | NULL | EUR 0 |
 Thyroid Cancer Exosome RNA |
| P241-TH | 101Bio | NULL | EUR 0 |
 ELISA Kit) Human Total PSA (t-PSA) ELISA Kit |
| PRB-5049-TOTAL | Cell Biolabs | 96 assays | EUR 572 |
 Navy RNA Lysis Kit 50 |
| NAVYE1-RNA | Next Advance | 50pack | EUR 198 |
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
 Navy RNA Lysis Kit 250 |
| NAVYE5-RNA | Next Advance | 250pack | EUR 651 |
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
 Green RNA Lysis Kit 50 |
| GREENE1-RNA | Next Advance | 50pack | EUR 180 |
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
 Green RNA Lysis Kit 250 |
| GREENE5-RNA | Next Advance | 250pack | EUR 571 |
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
 Pink RNA Lysis Kit 50 |
| PINKE1-RNA | Next Advance | 50pack | EUR 178 |
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 Pink RNA Lysis Kit 250 |
| PINKE5-RNA | Next Advance | 250pack | EUR 561 |
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 YELLOW RNA Lysis Kit 50 |
| YELLOWE1-RNA | Next Advance | 50pack | EUR 192 |
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 YELLOW RNA Lysis Kit 250 |
| YELLOWE5-RNA | Next Advance | 250pack | EUR 624 |
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 Red RNA Lysis Kit 50 |
| REDE1-RNA | Next Advance | 50pack | EUR 192 |
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 Red RNA Lysis Kit 250 |
| REDE5-RNA | Next Advance | 250pack | EUR 622 |
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 Exosome RNA) Diabetes (Type 1) Exosome RNA |
| P241-DB1 | 101Bio | NULL | EUR 0 |
 Exosome RNA) Diabetes (Type 2) Exosome RNA |
| P241-DB2 | 101Bio | NULL | EUR 0 |
 Leukemia - Hairy Cell Exosome RNA |
| P241-HC | 101Bio | NULL | EUR 0 |
 Leukemia - Multiple Myeloma Exosome RNA |
| P241-MM | 101Bio | NULL | EUR 0 |
 Exosome RNA) Melanoma (skin cancer) Exosome RNA |
| P241-MN | 101Bio | NULL | EUR 0 |
 Non-Hodgkin lymphoma Exosome RNA |
| P241-NH | 101Bio | NULL | EUR 0 |
 Systemic Lupus Erythematosus Exosome RNA |
| P241-SL | 101Bio | NULL | EUR 0 |
 Uterine Cervical Cancer Exosome RNA |
| P241-UC | 101Bio | NULL | EUR 0 |
