buffer a exosome protein extraction kit

Isolation Kit of Total Exosome RNA & Protein

The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum)).
• Extracts highly pure total RNA (including small RNA fraction) from exosomes
• Recovers protein and RNA from the same sample
• Compatible with all protocols for exosome isolation

The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). It is suitable for studies of RNA expression (specifically miRNA), processing, or function. The kit allows recovery of both RNA and proteins from the same purified exosome preparation. One portion of the sample is subjected to organic extraction, followed by immobilization of RNA on glass-fiber filters to purify total RNA or enrich for small RNA species. The other portion is then used directly for common protein applications such as Western blotting.

High yields of ultra-pure RNA can be prepared in about 30–60 minutes. The RNA can then be used in a variety of downstream applications, including qRT-PCR, high-throughput sequencing (e.g., using the Ion Torent™ PGM™, Ion Torrent Proton, or SOLiD sequencing instruments), microarray analysis, solution hybridization assays, and blot hybridization.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

buffer a exosome protein extraction kit

buffer a exosome protein extraction kit

exoEasy Maxi Kit

For purification of exosomes and other extracellular vesicles (EVs) from plasma, serum and cell culture supernatant

Features

 

  • Isolate intact exosomes and other extracellular vesicles
  • Suitable for functional testing and physical and biochemical analysis
  • Purify exosomes and other extracellular vesicles in 25 minutes
  • Process multiple samples in parallel using a simple spin-column procedure
  • Use with up to 4 ml of plasma or serum or 32 ml of cell culture supernatant
  • Suitable for MISEV-compliant protein characterization

Product Details

The exoEasy Maxi Kit uses membrane affinity spin columns to efficiently isolate exosomes and other extracellular vesicles from serum, plasma, cell culture supernatant and other biological fluids. The maxi column format allows the use of large sample volumes, including up to 4 ml plasma or serum or up to 32 ml cell culture supernatant. The procedure is very fast, consistent and highly suited for isolation of EVs for functional analysis of exosomes and other extracellular vesicles.

Performance

The exoEasy protocol for purification of exosomes and other extracellular vesicles is not only faster than ultracentrifugation, but also yields a cleaner preparation. Scanning electron microscopy shows that while both techniques deliver intact vesicles of the expected size, the preparation from ultracentrifugation also contains many smaller structures that do not match the expected size or shape for EVs (see figure “ Intact vesicles are eluted from the exoEasy membrane with higher purity compared with ultracentrifugation”).

Leukemia - ALL Exosome RNA

P241-ALL NULL
EUR 0

Leukemia - AML Exosome RNA

P241-AML NULL
EUR 0

Bladder Cancer Exosome RNA

P241-BL NULL
EUR 0

Breast Cancer Exosome RNA

P241-BR NULL
EUR 0

Leukemia - CLL Exosome RNA

P241-CLL NULL
EUR 0

Leukemia - CML Exosome RNA

P241-CML NULL
EUR 0

Colorectal Cancer Exosome RNA

P241-CO NULL
EUR 0

Crohn's Disease Exosome RNA

P241-CR NULL
EUR 0

Kidney Cancer Exosome RNA

P241-KN NULL
EUR 0

Lung Cancer Exosome RNA

P241-LG NULL
EUR 0

Liver Cancer Exosome RNA

P241-LV NULL
EUR 0

Nasopharyngeal Cancer Exosome RNA

P241-NA NULL
EUR 0

Prostate Cancer Exosome RNA

P241-PR NULL
EUR 0

Rheumatoid Arthritis Exosome RNA

P241-RA NULL
EUR 0

Thyroid Cancer Exosome RNA

P241-TH NULL
EUR 0

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL 96 assays
EUR 572

RNA isolater Total RNA Extraction Reagent

R401-01 100 ml
EUR 133

Green RNA Lysis Kit 50

GREENE1-RNA 50pack
EUR 180
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250

GREENE5-RNA 250pack
EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Navy RNA Lysis Kit 50

NAVYE1-RNA 50pack
EUR 198
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250

NAVYE5-RNA 250pack
EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Pink RNA Lysis Kit 50

PINKE1-RNA 50pack
EUR 178
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250

PINKE5-RNA 250pack
EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50

REDE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250

REDE5-RNA 250pack
EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50

YELLOWE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250

YELLOWE5-RNA 250pack
EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

LiqBiopsy Tumor Exosome RNA Kit

K1254-24
EUR 1110

Diabetes (Type 1) Exosome RNA

P241-DB1 NULL
EUR 0

Diabetes (Type 2) Exosome RNA

P241-DB2 NULL
EUR 0

Leukemia - Hairy Cell Exosome RNA

P241-HC NULL
EUR 0

Leukemia - Multiple Myeloma Exosome RNA

P241-MM NULL
EUR 0

Melanoma (skin cancer) Exosome RNA

P241-MN NULL
EUR 0

Non-Hodgkin lymphoma Exosome RNA

P241-NH NULL
EUR 0