buffer a exosome protein extraction kit

Isolation Kit of Total Exosome RNA & Protein

The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum)).
• Extracts highly pure total RNA (including small RNA fraction) from exosomes
• Recovers protein and RNA from the same sample
• Compatible with all protocols for exosome isolation

The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). It is suitable for studies of RNA expression (specifically miRNA), processing, or function. The kit allows recovery of both RNA and proteins from the same purified exosome preparation. One portion of the sample is subjected to organic extraction, followed by immobilization of RNA on glass-fiber filters to purify total RNA or enrich for small RNA species. The other portion is then used directly for common protein applications such as Western blotting.

High yields of ultra-pure RNA can be prepared in about 30–60 minutes. The RNA can then be used in a variety of downstream applications, including qRT-PCR, high-throughput sequencing (e.g., using the Ion Torent™ PGM™, Ion Torrent Proton, or SOLiD sequencing instruments), microarray analysis, solution hybridization assays, and blot hybridization.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

buffer a exosome protein extraction kit

buffer a exosome protein extraction kit

exoEasy Maxi Kit

For purification of exosomes and other extracellular vesicles (EVs) from plasma, serum and cell culture supernatant

Features

 

  • Isolate intact exosomes and other extracellular vesicles
  • Suitable for functional testing and physical and biochemical analysis
  • Purify exosomes and other extracellular vesicles in 25 minutes
  • Process multiple samples in parallel using a simple spin-column procedure
  • Use with up to 4 ml of plasma or serum or 32 ml of cell culture supernatant
  • Suitable for MISEV-compliant protein characterization

Product Details

The exoEasy Maxi Kit uses membrane affinity spin columns to efficiently isolate exosomes and other extracellular vesicles from serum, plasma, cell culture supernatant and other biological fluids. The maxi column format allows the use of large sample volumes, including up to 4 ml plasma or serum or up to 32 ml cell culture supernatant. The procedure is very fast, consistent and highly suited for isolation of EVs for functional analysis of exosomes and other extracellular vesicles.

Performance

The exoEasy protocol for purification of exosomes and other extracellular vesicles is not only faster than ultracentrifugation, but also yields a cleaner preparation. Scanning electron microscopy shows that while both techniques deliver intact vesicles of the expected size, the preparation from ultracentrifugation also contains many smaller structures that do not match the expected size or shape for EVs (see figure “ Intact vesicles are eluted from the exoEasy membrane with higher purity compared with ultracentrifugation”).

Lung Cancer Exosome RNA

P241-LG NULL Ask for price

Normal human exosome RNA

P240 NULL Ask for price

Leukemia - ALL Exosome RNA

P241-ALL NULL Ask for price

Leukemia - AML Exosome RNA

P241-AML NULL Ask for price

Leukemia - CLL Exosome RNA

P241-CLL NULL Ask for price

Leukemia - CML Exosome RNA

P241-CML NULL Ask for price

Liver Cancer Exosome RNA

P241-LV NULL Ask for price

Breast Cancer Exosome RNA

P241-BR NULL Ask for price

Crohn's Disease Exosome RNA

P241-CR NULL Ask for price

Kidney Cancer Exosome RNA

P241-KN NULL Ask for price

Bladder Cancer Exosome RNA

P241-BL NULL Ask for price

Thyroid Cancer Exosome RNA

P241-TH NULL Ask for price

Prostate Cancer Exosome RNA

P241-PR NULL Ask for price

Colorectal Cancer Exosome RNA

P241-CO NULL Ask for price

Rheumatoid Arthritis Exosome RNA

P241-RA NULL Ask for price

Nasopharyngeal Cancer Exosome RNA

P241-NA NULL Ask for price

Diabetes (Type 1) Exosome RNA

P241-DB1 NULL Ask for price

Diabetes (Type 2) Exosome RNA

P241-DB2 NULL Ask for price

Leukemia - Hairy Cell Exosome RNA

P241-HC NULL Ask for price

LiqBiopsy Tumor Exosome RNA Kit

K1254-24 each
EUR 1332

Melanoma (skin cancer) Exosome RNA

P241-MN NULL Ask for price

Uterine Corpus Cancer Exosome RNA

P241-UT NULL Ask for price

Non-Hodgkin lymphoma Exosome RNA

P241-NH NULL Ask for price

Uterine Cervical Cancer Exosome RNA

P241-UC NULL Ask for price

SeraMir Exosome RNA Ampflication Kit

RA800A-1 20 reactions
EUR 846

Leukemia - Multiple Myeloma Exosome RNA

P241-MM NULL Ask for price

Systemic Lupus Erythematosus Exosome RNA

P241-SL NULL Ask for price

Exosome RNA Helicase MTR4 (MTR4) Antibody

abx149899-100ug 100 ug
EUR 526.8
  • Shipped within 5-10 working days.

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

TotalExoRNA? Overall exosome immunocapture and RNA extraction kit (Biological Fluids/Cell Media, 20 reactions)

K1220-20 each
EUR 1462.8

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS NULL Ask for price

Exosome RNA Helicase MTR4 (MTR4/SKIV2L2) Antibody

20-abx004268
  • EUR 493.2
  • EUR 710.4
  • EUR 218.4
  • EUR 376.8
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set

RA810A-1 20 profiles
EUR 469

Rat SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set

RA812A-1 20 profiles
EUR 469