Recombinant Proteinase K RNases and DNases

Applications

• Recombinant proteinase K is extremely efficient on native proteins and therefore can be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.

• It is used for the analysis of membrane structures by modifying proteins and glycoproteins on the cell surface.

• Particularly suitable for isolating nucleic acids for amplification reactions.

• Promotes cell lysis by activating a bacterial autolytic factor.

• Used to remove cell debris during colony lift preparation and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.

Reconstitution Recommendations

Recombinant proteinase K is a nonspecific serine protease that is useful for general protein digestion.

Proteinase K is a highly reactive serine protease that shows the ability to digest native proteins, thus inactivating enzymes such as DNase and RNase without resorting to a denaturation process. It is the most potent proteinase among all the proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic, or hydrophobic amino acids.

Recombinant proteinase K is used in the isolation or preparation of high molecular weight nucleic acids. It is highly pure and has a higher specific activity and is more stable at room temperature compared to native proteinase K.

Recombinant proteinase K is expressed from the yeast Pichia pastoris with a cloned gene encoding the endolytic protease of Engyodontium album (Tritirachium album).

For the preparation of the stock solution, we suggest reconstituting the lyophilized powder in a buffer (20 mM Tris, 1 mM CaCl2, 2% glycerol, pH 7.4).

Proteinase K Recombinant Protein

Calcium is important for the thermostability of proteinase K and the pH value is critical for high solubility

The use of cryoprotectant glycerol to a final concentration of 50% in the stock solution is to prevent freeze / thaw damage. Proteinase K retained 99% of the activity after 12 freeze / thaw cycles in the stock solution. Avoid repeated freezing and thawing to avoid protein precipitation if glycerol is not added.

Proteinase K solution remains active in a wide pH range (4.0-12.5, often used in the 7.5-9.0 range) and in a wide temperature range of 25-75 ° C. The solution remains sterile and stable for at least 12 months at 4 ° C.

DNA / RNA Damage Antibody
abx440040-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx440321-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx440602-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx440883-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx441164-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx441445-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx441726-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx442007-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx442850-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx443130-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx443410-100ug	Abbexa	100 ug	EUR 644.4
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx443691-100ug	Abbexa	100 ug	EUR 678
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx443972-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx442288-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

DNA / RNA Damage Antibody
abx442569-100ug	Abbexa	100 ug	EUR 661.2
Shipped within 5-12 working days.

Monoclonal antibody for DNA/RNA Damage
SMC-155C	Stressmarq	0.025mg	EUR 228
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

Monoclonal antibody for DNA/RNA Damage
SMC-155D	Stressmarq	0.1mg	EUR 398.4
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is not conjugated.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A390	Stressmarq	0.1mg	EUR 454.8
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Dog | Rabbit DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 390.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A488	Stressmarq	0.1mg	EUR 453.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 488.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A565	Stressmarq	0.1mg	EUR 453.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 565.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A594	Stressmarq	0.1mg	EUR 453.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 594.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A633	Stressmarq	0.1mg	EUR 453.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 633.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A655	Stressmarq	0.1mg	EUR 453.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 655.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A680	Stressmarq	0.1mg	EUR 453.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 680.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-A700	Stressmarq	0.1mg	EUR 453.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with ATTO 700.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-ALP	Stressmarq	0.1mg	EUR 446.4
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Alkaline Phosphatase.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-APC	Stressmarq	0.1mg	EUR 452.4
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-APCCY7	Stressmarq	0.1mg	EUR 540
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with APC/Cy7.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-BI	Stressmarq	0.1mg	EUR 448.8
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Biotin.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY350	Stressmarq	0.1mg	EUR 471.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 350.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY405	Stressmarq	0.1mg	EUR 457.2
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 405.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY488	Stressmarq	0.1mg	EUR 445.2
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 488.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY594	Stressmarq	0.1mg	EUR 447.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 594.

Monoclonal antibody for DNA/RNA Damage
SMC-155D-DY633	Stressmarq	0.1mg	EUR 441.6
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are Show more
Description: A monoclonal antibody from clone 15A3 against Human | Mouse | Rat | Bovine | Monkey | Rabbit | Pig | Dog | Hamster | Chicken | Sheep | Guinea Pig (Cavia porcellus) DNA/RNA Damage. The host species for the production of this antibody is Mouse. The antigen used for immunization is 8-hydroxy-guanosine-BSA and ?casein conjugates. The antibody is tested and validated for IHC, ICC/IF, ELISA, DB, IP, FCM, FA assays with the following recommended dilutions: IHC (1:1000). This MAb for DNA/RNA Damage is conjugated with Dylight 633.