purofied native dsDNA

DNA preferentially bind DNA presented on PolIV

ABSTRACT
While immunoglobulin G (IgG) antibodies against double-stranded (ds) DNA are serological markers of systemic lupus erythematosus (SLE), not all anti-DNA (anti-DNA) antibodies are positively damaged. It has been suggested that anti-DNA-induced renal damage may be associated with differences in the fine specificity of antibodies. In an effort to gain insight into their fine binding properties, we investigated the cross-reactivity of two human lupus anti-dsDNA monoclonal IgGs (B3 to RH14) to the recently described PolIV. These autoantibodies have distinct pathogenic properties in mice with severe combined immunodeficiency (SCID). Although both antibodies cause proteinuria, only RH14 induces the early histological features of lupus nephritis.

Both RH14 and B3 bound PolIV; however, they showed a significant difference in their reactivity to the PolIV – dsDNA complex. Although RH14 showed significant activity to the complex, the binding of B3 to PolIV complexed with dsDNA was almost abolished. In addition, there was a significant difference in the way lupus is recognized by naked dsDNA and which is presented on PolIV. Although 67% of lupus sera bound to bare dsDNA, ≈ 90% of these sera (93% calf thymus DNA; 90% synthetic oligonucleotide) responded to the complex when the dsDNA was presented on PolIV.

purofied native dsDNA

purofied native dsDNA

Thus, anti-dsDNA IgG, which is likely to exist in lupus patients, can be distinguished between those that recognize dsDNA in the context of PolIV and those that do not. This difference in binding ability can help differentiate those dsDNA antibodies that are more pathogenic.

Key words: autoantibodies, autoimmunity, human, lupus, PolIV

 

ADDITIONAL INFORMATION
Product NamePurified Native dsDNA, Calf Thymus, ELISA/Antigen grade
Supplier nameAlpha Diagnostic
Size1 mg
FormPowder
Datasheet URLView Datasheet
Product TypeAntibody Purification
PublicationsPublication List

Anti-dsDNA antibodies anti-ssDNA antibodies

2.1. Anti-DNA antibodies and their correlation with the pathogenesis of SLE
Systemic lupus erythematosus (SLE) is a chronic, potentially fatal autoimmune disease characterized by exacerbations and remissions with a variety of clinical manifestations affecting multiple organ systems, including the skin, kidneys, joints, cardiovascular and nervous systems.

A hallmark of systemic lupus erythematosus is the production of a number of IgG and IgM autoantibodies directed against one or more nuclear components, the most common of which are double-stranded (ds) DNA and / or single-stranded (ss) DNA. Both anti-ssDNA and anti-dsDNA are involved in disease development and have been eluted from the kidneys of experimental mouse models and patients with SLE [17]. Clinicians consider anti-dsDNA autoantibodies to be relatively disease-specific, while anti-ssDNA is non-specific.

The reason for the difference in disease specificity is that for antibodies against dsDNA, the sensitivity of the test is 60%, while for these antibodies the variability is 30-70% [18]. The level of anti-DNA antibodies varies in the plasma of different patients with SLE, with high levels of anti-ssDNA and / or anti-dsDNA antibodies being associated with flare-ups of symptoms [4, 5]. As a result, the level of anti-DNA antibodies in the serum of patients is used to monitor the activity and progression of the disease [4-6].

The exact mechanisms leading to the production of anti-DNA antibodies remain unknown. Subgroups of B-cell producers vary according to different authors [19-21]. In addition, the mechanisms of pathogenicity of anti-DNA antibodies and immune complexes in SLE are controversial.

Part of the pathogenicity may be due to the direct hydrolytic and cytotoxic activity of anti-DNA antibodies on cells of various tissues and organs affected by the disease [18]. Pathogenic anti-DNA antibodies are able to interact at the tissue level with alpha actinin in the renal glomeruli.

At the cellular level, antibodies have been shown to react with various cell surface proteins (eg, Myosin 1), presumably to allow them to penetrate the cell [22–25]. Upon entering the cell, the anti-DNA antibody is translocated to the nucleus, where it binds to the DNA and subsequently hydrolyzes it. An alternative mechanism by which an anti-DNA antibody may lead to autoimmune disease is its interaction with cell death receptors, which initiates a proapoptotic signal that apparently leads to tumor cell death in vitro and possibly lupus B-cell death in vivo [12, 15 , 26, 27].

Although this phenomenon is due in part to caspase activation, the possible link between DNA hydrolysis and programmed cell death, as well as the underlying mechanisms in SLE, are not clearly understood [12]. The differences between natural and pathogenic anti-DNA autoantibodies are summarized in Figure 1.

Preferentially Expressed Antigen In Melanoma (PRAME) Antibody

20-abx104373
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Human Preferentially Expressed Antigen In Melanoma ELISA Kit

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Description: Human Preferentially Expressed Antigen In Melanoma ELISA Kit

Melanoma antigen preferentially expressed in tumors Antibody

20-abx109324
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Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)

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Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)

4-RPH323Hu02
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  • Predicted Molecular Mass (KD): 36.9kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Preferentially Expressed Antigen In Melanoma expressed in: E.coli

Human Preferentially Expressed Antigen In Melanoma (PRAME) Protein

20-abx068652
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Human Preferentially Expressed Antigen In Melanoma (PRAME) Protein

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1-CSB-YP018603HU
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Description: Recombinant Human Melanoma antigen preferentially expressed in tumors(PRAME) expressed in Yeast

Human Melanoma antigen preferentially expressed in tumors (PRAME)

1-CSB-EP018603HU
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  • MW: 61.9 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Melanoma antigen preferentially expressed in tumors(PRAME) expressed in E.coli

Human Preferentially Expressed Antigen In Melanoma (PRAME) CLIA Kit

20-abx495430
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Melanoma Antigen Preferentially Expressed In Tumors (MAPE) Antibody

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20-abx004948
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Human Striated muscle preferentially expressed protein kinase (SPEG)

1-CSB-EP022529HU
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  • MW: 38.7 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Striated muscle preferentially expressed protein kinase(SPEG),partial expressed in E.coli

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

DLR-PRAME-Hu-48T 48T
EUR 620.4
  • Should the Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Preferentially Expressed Antigen In Melanoma (PRAME) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

DLR-PRAME-Hu-96T 96T
EUR 807.6
  • Should the Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Preferentially Expressed Antigen In Melanoma (PRAME) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

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Human Preferentially Expressed Antigen In Melanoma ELISA Kit (PRAME)

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Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

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Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

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Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

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Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

DLR-PRAME-Hu 96T
EUR 463
  • 0.105ng/mL
Description: tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

DL-PRAME-Hu 96T
EUR 441
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Description: tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

SEH323Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
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  • CV(%) = SD/meanX100
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Preferentially Expressed Antigen In Melanoma (PRAME) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

SEH323Hu-1x48wellstestplate 1x48-wells test plate
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  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Preferentially Expressed Antigen In Melanoma (PRAME) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<1
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Preferentially Expressed Antigen In Melanoma (PRAME) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

SEH323Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Preferentially Expressed Antigen In Melanoma (PRAME) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<1
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Preferentially Expressed Antigen In Melanoma (PRAME) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

SEH323Hu-5x96wellstestplate 5x96-wells test plate
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  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Preferentially Expressed Antigen In Melanoma (PRAME) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<1
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Preferentially Expressed Antigen In Melanoma (PRAME) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

4-SEH323Hu
  • EUR 5738.4
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  • EUR 768
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Preferentially Expressed Antigen In Melanoma elisa. Alternative names of the recognized antigen: MAPE
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  • CT130
  • Cancer/Testis Antigen 130
  • Opa-interacting protein 4
  • Melanoma antigen preferentially expressed in tumors
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Preferentially Expressed Antigen In Melanoma (PRAME) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

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Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

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EUR 679.04
  • sandwich
Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

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EUR 452.7
  • sandwich
Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

RD-PRAME-Hu-96T 96T
EUR 646.7
  • sandwich
Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

ELISA kit for Human PRAME (Preferentially Expressed Antigen In Melanoma)

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EUR 518.4
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Preferentially Expressed Antigen In Melanoma (PRAME). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated an
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Description: A sandwich ELISA kit for detection of Preferentially Expressed Antigen In Melanoma from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Dog Striated muscle preferentially expressed protein kinase ELISA kit

E08S0465-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Canine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Striated muscle preferentially expressed protein kinase ELISA kit

E08S0465-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Canine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Striated muscle preferentially expressed protein kinase ELISA kit

E08S0465-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Canine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Striated muscle preferentially expressed protein kinase ELISA kit

E02S0465-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rat Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Striated muscle preferentially expressed protein kinase ELISA kit

E02S0465-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rat Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Striated muscle preferentially expressed protein kinase ELISA kit

E02S0465-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Rat Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Striated muscle preferentially expressed protein kinase ELISA kit

E07S0465-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Porcine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Striated muscle preferentially expressed protein kinase ELISA kit

E07S0465-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Porcine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Striated muscle preferentially expressed protein kinase ELISA kit

E07S0465-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Porcine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Melanoma Antigen Preferentially Expressed In Tumors (PRAME) Antibody (FITC)

20-abx107068
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  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
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  • 20 ug
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  • Shipped within 5-10 working days.

Melanoma Antigen Preferentially Expressed In Tumors (PRAME) Antibody (HRP)

20-abx108489
  • EUR 493.2
  • EUR 2214
  • EUR 718.8
  • EUR 218.4
  • EUR 360
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.