purofied native dsDNA

DNA preferentially bind DNA presented on PolIV

ABSTRACT
While immunoglobulin G (IgG) antibodies against double-stranded (ds) DNA are serological markers of systemic lupus erythematosus (SLE), not all anti-DNA (anti-DNA) antibodies are positively damaged. It has been suggested that anti-DNA-induced renal damage may be associated with differences in the fine specificity of antibodies. In an effort to gain insight into their fine binding properties, we investigated the cross-reactivity of two human lupus anti-dsDNA monoclonal IgGs (B3 to RH14) to the recently described PolIV. These autoantibodies have distinct pathogenic properties in mice with severe combined immunodeficiency (SCID). Although both antibodies cause proteinuria, only RH14 induces the early histological features of lupus nephritis.

Both RH14 and B3 bound PolIV; however, they showed a significant difference in their reactivity to the PolIV – dsDNA complex. Although RH14 showed significant activity to the complex, the binding of B3 to PolIV complexed with dsDNA was almost abolished. In addition, there was a significant difference in the way lupus is recognized by naked dsDNA and which is presented on PolIV. Although 67% of lupus sera bound to bare dsDNA, ≈ 90% of these sera (93% calf thymus DNA; 90% synthetic oligonucleotide) responded to the complex when the dsDNA was presented on PolIV.

purofied native dsDNA

purofied native dsDNA

Thus, anti-dsDNA IgG, which is likely to exist in lupus patients, can be distinguished between those that recognize dsDNA in the context of PolIV and those that do not. This difference in binding ability can help differentiate those dsDNA antibodies that are more pathogenic.

Key words: autoantibodies, autoimmunity, human, lupus, PolIV

 

ADDITIONAL INFORMATION
Product NamePurified Native dsDNA, Calf Thymus, ELISA/Antigen grade
Supplier nameAlpha Diagnostic
Size1 mg
FormPowder
Datasheet URLView Datasheet
Product TypeAntibody Purification
PublicationsPublication List

Anti-dsDNA antibodies anti-ssDNA antibodies

2.1. Anti-DNA antibodies and their correlation with the pathogenesis of SLE
Systemic lupus erythematosus (SLE) is a chronic, potentially fatal autoimmune disease characterized by exacerbations and remissions with a variety of clinical manifestations affecting multiple organ systems, including the skin, kidneys, joints, cardiovascular and nervous systems.

A hallmark of systemic lupus erythematosus is the production of a number of IgG and IgM autoantibodies directed against one or more nuclear components, the most common of which are double-stranded (ds) DNA and / or single-stranded (ss) DNA. Both anti-ssDNA and anti-dsDNA are involved in disease development and have been eluted from the kidneys of experimental mouse models and patients with SLE [17]. Clinicians consider anti-dsDNA autoantibodies to be relatively disease-specific, while anti-ssDNA is non-specific.

The reason for the difference in disease specificity is that for antibodies against dsDNA, the sensitivity of the test is 60%, while for these antibodies the variability is 30-70% [18]. The level of anti-DNA antibodies varies in the plasma of different patients with SLE, with high levels of anti-ssDNA and / or anti-dsDNA antibodies being associated with flare-ups of symptoms [4, 5]. As a result, the level of anti-DNA antibodies in the serum of patients is used to monitor the activity and progression of the disease [4-6].

The exact mechanisms leading to the production of anti-DNA antibodies remain unknown. Subgroups of B-cell producers vary according to different authors [19-21]. In addition, the mechanisms of pathogenicity of anti-DNA antibodies and immune complexes in SLE are controversial.

Part of the pathogenicity may be due to the direct hydrolytic and cytotoxic activity of anti-DNA antibodies on cells of various tissues and organs affected by the disease [18]. Pathogenic anti-DNA antibodies are able to interact at the tissue level with alpha actinin in the renal glomeruli.

At the cellular level, antibodies have been shown to react with various cell surface proteins (eg, Myosin 1), presumably to allow them to penetrate the cell [22–25]. Upon entering the cell, the anti-DNA antibody is translocated to the nucleus, where it binds to the DNA and subsequently hydrolyzes it. An alternative mechanism by which an anti-DNA antibody may lead to autoimmune disease is its interaction with cell death receptors, which initiates a proapoptotic signal that apparently leads to tumor cell death in vitro and possibly lupus B-cell death in vivo [12, 15 , 26, 27].

Although this phenomenon is due in part to caspase activation, the possible link between DNA hydrolysis and programmed cell death, as well as the underlying mechanisms in SLE, are not clearly understood [12]. The differences between natural and pathogenic anti-DNA autoantibodies are summarized in Figure 1.

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Preferentially Expressed Antigen In Melanoma (PRAME) Antibody

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Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)

4-RPH323Hu02
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Human Melanoma antigen preferentially expressed in tumors (PRAME)

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Description: Recombinant Human Melanoma antigen preferentially expressed in tumors(PRAME) expressed in E.coli

Human Striated muscle preferentially expressed protein kinase (SPEG)

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  • MW: 38.7 kDa
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Description: Recombinant Human Striated muscle preferentially expressed protein kinase(SPEG),partial expressed in E.coli

Human Melanoma antigen preferentially expressed in tumors (PRAME)

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  • MW: 59.9 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Melanoma antigen preferentially expressed in tumors(PRAME) expressed in Yeast

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Description: A sandwich quantitative ELISA assay kit for detection of Human Preferentially Expressed Antigen In Melanoma (PRAME) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Preferentially Expressed Antigen In Melanoma (PRAME) ELISA Kit

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Description: A sandwich quantitative ELISA assay kit for detection of Human Preferentially Expressed Antigen In Melanoma (PRAME) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

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E04S0465-192T 192 tests
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  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Rabbit Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Striated muscle preferentially expressed protein kinase ELISA kit

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Description: A sandwich ELISA for quantitative measurement of Rabbit Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Striated muscle preferentially expressed protein kinase ELISA kit

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  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Rabbit Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Striated muscle preferentially expressed protein kinase ELISA kit

E02S0465-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Rat Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Striated muscle preferentially expressed protein kinase ELISA kit

E02S0465-48 1 plate of 48 wells
EUR 520
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Description: A sandwich ELISA for quantitative measurement of Rat Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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E02S0465-96 1 plate of 96 wells
EUR 685
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Description: A sandwich ELISA for quantitative measurement of Rat Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Striated muscle preferentially expressed protein kinase ELISA kit

E03S0465-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Mouse Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Striated muscle preferentially expressed protein kinase ELISA kit

E03S0465-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Mouse Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Striated muscle preferentially expressed protein kinase ELISA kit

E03S0465-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Mouse Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Striated muscle preferentially expressed protein kinase ELISA kit

E01S0465-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Human Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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E01S0465-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Human Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Striated muscle preferentially expressed protein kinase ELISA kit

E01S0465-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Human Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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20-abx105651
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EUR 1270
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Description: A sandwich ELISA for quantitative measurement of Canine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Striated muscle preferentially expressed protein kinase ELISA kit

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Description: A sandwich ELISA for quantitative measurement of Canine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Striated muscle preferentially expressed protein kinase ELISA kit

E08S0465-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Canine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Striated muscle preferentially expressed protein kinase ELISA kit

E07S0465-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Porcine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Striated muscle preferentially expressed protein kinase ELISA kit

E07S0465-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Porcine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Striated muscle preferentially expressed protein kinase ELISA kit

E07S0465-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Porcine Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Striated muscle preferentially expressed protein kinase ELISA kit

E09S0465-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Monkey Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Striated muscle preferentially expressed protein kinase ELISA kit

E09S0465-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Monkey Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Striated muscle preferentially expressed protein kinase ELISA kit

E09S0465-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Monkey Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Striated muscle preferentially expressed protein kinase ELISA kit

E06S0465-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Goat Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Striated muscle preferentially expressed protein kinase ELISA kit

E06S0465-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Goat Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Striated muscle preferentially expressed protein kinase ELISA kit

E06S0465-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A sandwich ELISA for quantitative measurement of Goat Striated muscle preferentially expressed protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Preferentially Expressed Antigen In Melanoma (PRAME) CLIA Kit

20-abx495430
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.