WeFaceNano: a user-friendly pipeline for complete ONT sequence assembly and detection of antibiotic resistance in multi-plasmid bacterial isolates

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Background: Bacterial plasmids typically carry antibiotic resistance genes and are a big issue within the unfold of antibiotic resistance. The flexibility to utterly assemble plasmid sequences would facilitate the localization of antibiotic resistance genes, the identification of genes that promote plasmid transmission and the correct monitoring of plasmid mobility. Nonetheless, the whole meeting of plasmid sequences utilizing the at present most generally used sequencing platform (Illumina-based sequencing) is restricted as a result of era of quick sequence lengths. The long-read Oxford Nanopore Applied sciences (ONT) sequencing platform overcomes this limitation. Nonetheless, the meeting of plasmid sequence knowledge stays difficult resulting from software program incompatibility with long-reads and the error fee generated utilizing ONT sequencing. Bioinformatics pipelines have been developed for ONT-generated sequencing however require computational expertise that regularly are past the skills of scientific researchers.

 

To beat this problem, the authors developed ‘WeFaceNano’, a user-friendly Net interFace for fast meeting and evaluation of plasmid DNA sequences generated utilizing the ONT platform. WeFaceNano contains: a learn statistics report; two assemblers (Miniasm and Flye); BLAST looking out; the detection of antibiotic resistance- and replicon genes and several other plasmid visualizations. A user-friendly interface shows the primary options of WeFaceNano and offers entry to the evaluation instruments.

Outcomes: Publicly out there ONT sequence knowledge of 21 plasmids have been used to validate WeFaceNano, with plasmid assemblages and anti-microbial resistance gene detection being concordant with the printed outcomes. Curiously, the “Flye” assembler with “meta” settings generated essentially the most full plasmids.

Conclusions: WeFaceNano is a user-friendly open-source software program pipeline appropriate for correct plasmid meeting and the detection of anti-microbial resistance genes in (scientific) samples the place a number of plasmids could be current.

Antibiotic resistance and plasmid evaluation of Enterobacteriaceae remoted from retail meat in Lagos Nigeria

 

Background: The presence of antibiotic resistant microorganisms in meals is of nice concern globally. This analysis was carried out to detect and characterize plasmid carriage and profiles amongst members of Enterobacteriaceae from completely different meat sorts in Nigeria.

Technique: From a complete of 80 meat samples comprising of mutton,pork, beef and rooster, organisms belonging to the household Enterobacteriaceae wereisolated by commonplace procedures and recognized by API 20E system. Antibiotics susceptibilities testing (AST) againstselected courses of antimicrobial brokers and plasmid extraction was carried outby disc diffusion and alkaline lysis strategies respectively.

Outcomes: One-hundred and ten Enterobacteriaceae have been remoted,species identification revealed isolates belonging to 7 genera comprising of Escherichia, Enterobacter, Klebsiella,Citrobacter, Proteus, Salmonella and Serratia. Total resistance of theorganisms to amoxycillin/clavulanic acid was 91 (82.7%), streptomycin 85(75.7%) and perfloxacin 74 (67.2%) whereas ofloxacin had the highestsusceptibility fee (91.8%). Plasmids profiling revealed ranges of plasmids from1 to three copies with estimated sizes vary of 700bp to 1.1kb amongst E. coli, Okay. pneumoniae, E. aerogenesand Proteus mirabilis. All theisolates with plasmids have been multidrug resistant and have been remoted from rooster excepta pressure of E. coli from pork whichharboured a single plasmid copy suggesting these meat as reservoirs forantibiotic resistant micro organism.

Conclusion: Our findings revealed excessive stage of meat contamination with antibioticresistant Enterobacteriaceae harbouring resistant plasmids. An integratedsurveillance system and security follow should be ensured among the many processorsand retailers.

Identification of a novel plasmid-mediated carbapenemase-encoding gene blaVMB-2 in Vibrio diabolicus

 

We characterised a carbapenem-resistant Vibrio diabolicus pressure of shrimp origin with numerous experiments and bioinformatics evaluation. A novel metallo-β-lactamase gene blaVMB-2, conferring resistance to β-lactams together with meropenem and cephalosporins, was recognized on a plasmid-borne composite transposon ISShfr9-ISCR1blaVMB-2blaCARB-12aadA1-ISShfr9 able to producing blaVMB-2-bearing round intermediate. ISShfr9 was discovered disseminated on MDR pathogens, arousing the priority of additional transmission of blaVMB-2-bearing round intermediate to scientific Enterobacterales through such insertion sequence, which warrants additional investigations.

Outcomes of Dynamic Distinction-Enhanced Ultrasound Correlate With Therapy Final result in Canine Neoplasia Handled With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer

 

Electrochemotherapy (ECT) and/or gene electrotransfer of plasmid DNA encoding interleukin-12 (GET pIL-12) are efficient remedies for canine cutaneous, subcutaneous, and maxillofacial tumors. Regardless of the scientific efficacy of the mixed remedies of ECT and GET, knowledge on parameters which may predict the result of the remedies are nonetheless missing. This research aimed to research whether or not dynamic contrast-enhanced ultrasound (DCE-US) outcomes of subcutaneous tumors differ between tumors with full response (CR) and tumors with out full response (non-CR) in canine handled with ECT and GET pIL-12. Eight canine with a complete of 12 tumor nodules handled with ECT and GET pIL-12 have been included. DCE-US examinations have been carried out in all animals earlier than and instantly after remedy in addition to Eight h and 1, 3, and seven days later.

Medical follow-up examinations have been carried out 7 and 14 days, 1 and 6 months, and 1 yr after therapy. Quite a few vital variations in DCE-US parameters have been famous between tumors with CR and non-CR tumors; perfusion and perfusion heterogeneity have been decrease in CR tumors than in non-CR tumors. Due to this fact, research with bigger numbers of sufferers are wanted to research whether or not DCE-US outcomes can be utilized to foretell therapy outcomes and to make efficient choices in regards to the want for repeated remedy or completely different therapy mixtures in particular person sufferers.

heraeus-targets

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Excessive-throughput analysis of polymeric nanoparticles for tissue-targeted gene expression utilizing barcoded plasmid DNA

More and more, analysis laboratories are fabricating libraries of novel nanoparticles, engineering each new biomaterial constructions and composition ratios of multicomponent methods. But, strategies for screening gene supply automobiles straight in vivo are typically low-throughout, limiting the variety of candidate nanoparticles that may be investigated. Right here, we report a complete, high-throughput technique to judge a library of polymeric nanoparticles in vivo for tissue-specific gene supply. The tactic entails pairing every nanoparticle formulation with a plasmid DNA (pDNA) that harbors a novel nucleotide sequence serving because the figuring out “barcode”. Utilizing actual time quantitative PCR (qPCR) for detection of the barcoded pDNA and quantitative reverse transcription PCR (RT-qPCR) for transcribed barcoded mRNA, we are able to quantify accumulation and transfection in tissues of curiosity. The barcode pDNA and primers have been designed with adequate sensitivity and specificity to judge a number of nanoparticle formulations per mouse, enhancing screening effectivity.

Utilizing this platform, we evaluated the biodistribution and transfection of Eight intravenously administered poly(beta-amino ester) (PBAE) nanoparticle formulations, every with a PBAE polymer of differential construction. Important ranges of nanoparticle accumulation and gene transfection have been noticed primarily in organs concerned in clearance, together with spleen, liver, and kidneys. Curiously, increased ranges of transfection of choose organs didn’t essentially correlate with increased ranges of tissue accumulation, highlighting the significance of straight measuring in vivo transfection effectivity as the important thing barcoded parameter in gene supply vector optimization. To validate this technique, nanoparticle formulations have been used individually for luciferase pDNA supply in vivo. The distribution of luciferase expression in tissues matched the transfection evaluation by the barcode qPCR technique, confirming that this platform can be utilized to precisely consider systemic gene supply.

 

RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 25-36

KS073012-III 1 Kit
EUR 1142
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 25-36

KS074012-III 1 Kit
EUR 1021
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody

K3171120-III 1200 cm2
EUR 246
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody

K3171250-III 2500 cm2
EUR 310
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Mounting Medium

4300 3 ml
EUR 180.5
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

VALI Medium

450 500 ml
EUR 265

BEGM Medium

451 500 ml
EUR 152

DC MEDIUM

D04-117-10kg 10 kg
EUR 1579

DC MEDIUM

D04-117-2kg 2kg
EUR 382

DC MEDIUM

D04-117-500g 500 g
EUR 140

EC MEDIUM

E05-100-10kg 10 kg
EUR 814

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 216

EC MEDIUM

E05-100-500g 500 g
EUR 95

Advanced Medium

C0003-04 RT 500 mL Bottle
EUR 103

Medium 199

C0012-01 RT 500 mL Bottle
EUR 105

COLIFORM MEDIUM

C03-127-10kg 10 kg
EUR 1324

COLIFORM MEDIUM

C03-127-2kg 2kg
EUR 327

COLIFORM MEDIUM

C03-127-500g 500 g
EUR 125

Heller's Medium

CP014-010 10X1L
EUR 99

Heller's Medium

CP014-500 50L
EUR 126

Hoagland's Medium

CP015-010 10X1L
EUR 99

Hoagland's Medium

CP015-500 50L
EUR 126

HLP MEDIUM

H08-107-10kg 10 kg
EUR 2278

HLP MEDIUM

H08-107-2Kg 2 Kg
EUR 534

HLP MEDIUM

H08-107-500g 500 g
EUR 182

NeuroProgenitor Medium

NM42400 125 ml
EUR 304

SIM MEDIUM

S19-110-10kg 10 kg
EUR 1021

SIM MEDIUM

S19-110-2kg 2kg
EUR 261

SIM MEDIUM

S19-110-500g 500 g
EUR 107

SOB MEDIUM

S19-124-10kg 10 kg
EUR 965

SOB MEDIUM

S19-124-2kg 2kg
EUR 249

SOB MEDIUM

S19-124-500g 500 g
EUR 104

A Medium

DJ1018 100g
EUR 84.8
  • Product category: Culture Media/Medium

M9 Medium

SD7024 250g
EUR 71.75
  • Product category: Culture Media/Medium

M9CA Medium

SD7025 250g
EUR 71.75
  • Product category: Culture Media/Medium

YM Medium

SD7031 250g
EUR 70.45
  • Product category: Culture Media/Medium

TYGPN Medium

SD7032 250g
EUR 70.01
  • Product category: Culture Media/Medium

M63 Medium

SD7033 250g
EUR 71.75
  • Product category: Culture Media/Medium

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 71

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 483
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 249
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 385
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 120.00
  • EUR 80.00
  • 1L
  • 500mL


  • Working procedure:
    1) Invert the bottle a few times to ensure the solution is properly suspended.
    2) Take the gel out of the gel tank and submerse the gel in enough SDS-Blue to cover the gel.
    3) Bands will be visi
  • Show more
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)

ABP-MED-10004 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:

EverBrite Mounting Medium

23001 10mL
EUR 178
Description: Minimum order quantity: 1 unit of 10mL

Recovery Medium - 6x12mL

1711 4/PK
EUR 128

SuperMOUNT? Mounting Medium

1211-20
EUR 201

Aqueous Mounting Medium

AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 80

Antifade Mounting Medium

AR1109 10mL (for 1000 assays)
EUR 65

EC MEDIUM, MODIFIED

E05-108-10kg 10 kg
EUR 858

EC MEDIUM, MODIFIED

E05-108-2Kg 2 Kg
EUR 226

EC MEDIUM, MODIFIED

E05-108-500g 500 g
EUR 98

A-1 MEDIUM

A01-116-10kg 10 kg
EUR 1034

A-1 MEDIUM

A01-116-2Kg 2 Kg
EUR 264

A-1 MEDIUM

A01-116-500g 500 g
EUR 108

RPMI 1640 Medium

abx098879-500ml 500 ml
EUR 175
  • Shipped within 5-10 working days.

MCDB 131 Medium

CM034-050 500ml
EUR 85

MCDB 131 Medium

CM034-300 6x500ml
EUR 165

MCDB 131 Medium

CM034-310 10x500ml
EUR 219

MCDB 131 Medium

CM034-320 20x500ml
EUR 340

MCDB 131 Medium

CM034-350 50x500ml
EUR 585

Williams' Medium E

CM063-050 500ml
EUR 91

Williams' Medium E

CM063-300 6x500ml
EUR 266

Williams' Medium E

CM063-310 10x500ml
EUR 313

Williams' Medium E

CM063-320 20x500ml
EUR 604

Williams' Medium E

CM063-350 50x500ml
EUR 975

AddexBio Cryopreservation Medium

C0002-20mL RT vial
EUR 104

CARY & BLAIR MEDIUM

C03-131-10kg 10 kg
EUR 2185

CARY & BLAIR MEDIUM

C03-131-2Kg 2 Kg
EUR 514

CARY & BLAIR MEDIUM

C03-131-500g 500 g
EUR 176

Cereal Crops Medium

CP003-005 5L
EUR 113

Cereal Crops Medium

CP003-010 10X1L
EUR 169

Kao Michayluk Medium

CP017-010 10X1L
EUR 99

Kao Michayluk Medium

CP017-500 50L
EUR 126

Orchid Maintenance Medium

CP037-010 10X1L
EUR 109

Orchid Maintenance Medium

CP037-500 50L
EUR 138

Woody Plants Medium

CP040-010 10X1L
EUR 101

Woody Plants Medium

CP040-500 50L
EUR 126

Hepatocyte Maintenance Medium

HM42600 100 ml
EUR 409

Neuron Growth Medium

HNM001 500 ml
EUR 331

FLUID THIOGLYCOLLATE MEDIUM

F06-101-10kg 10 kg
EUR 792

FLUID THIOGLYCOLLATE MEDIUM

F06-101-2kg 2kg
EUR 211

FLUID THIOGLYCOLLATE MEDIUM

F06-101-500g 500 g
EUR 94

Agarose, medium EEO

GE4750-100G 100 g
EUR 166

Agarose, medium EEO

GE4750-500G 500 g
EUR 429

FluoreGuard Mounting Medium

FMM030 30 ml
EUR 96

FluoreGuard Mounting Medium

FMM060 60 ml
EUR 116
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Effects of Emicizumab on APTT, FVIII assays and FVIII Inhibitor assays using different reagents: Results of a UK NEQAS proficiency testing exerciseEffects of Emicizumab on APTT, FVIII assays and FVIII Inhibitor assays using different reagents: Results of a UK NEQAS proficiency testing exercise

Introduction: Emicizumab (Hemlibra: Roche Switzerland) is a, humanized, bi-specific monoclonal modified immunoglobulin G4 (IgG4) which binds human FX, FIX and activated FIX (FIXa) to imitate activated FVIII exercise. Intention: Consider the consequences