WeFaceNano: a user-friendly pipeline for complete ONT sequence assembly and detection of antibiotic resistance in multi-plasmid bacterial isolates

heraeus-targets

Background: Bacterial plasmids typically carry antibiotic resistance genes and are a big issue within the unfold of antibiotic resistance. The flexibility to utterly assemble plasmid sequences would facilitate the localization of antibiotic resistance genes, the identification of genes that promote plasmid transmission and the correct monitoring of plasmid mobility. Nonetheless, the whole meeting of plasmid sequences utilizing the at present most generally used sequencing platform (Illumina-based sequencing) is restricted as a result of era of quick sequence lengths. The long-read Oxford Nanopore Applied sciences (ONT) sequencing platform overcomes this limitation. Nonetheless, the meeting of plasmid sequence knowledge stays difficult resulting from software program incompatibility with long-reads and the error fee generated utilizing ONT sequencing. Bioinformatics pipelines have been developed for ONT-generated sequencing however require computational expertise that regularly are past the skills of scientific researchers.

 

To beat this problem, the authors developed ‘WeFaceNano’, a user-friendly Net interFace for fast meeting and evaluation of plasmid DNA sequences generated utilizing the ONT platform. WeFaceNano contains: a learn statistics report; two assemblers (Miniasm and Flye); BLAST looking out; the detection of antibiotic resistance- and replicon genes and several other plasmid visualizations. A user-friendly interface shows the primary options of WeFaceNano and offers entry to the evaluation instruments.

Outcomes: Publicly out there ONT sequence knowledge of 21 plasmids have been used to validate WeFaceNano, with plasmid assemblages and anti-microbial resistance gene detection being concordant with the printed outcomes. Curiously, the “Flye” assembler with “meta” settings generated essentially the most full plasmids.

Conclusions: WeFaceNano is a user-friendly open-source software program pipeline appropriate for correct plasmid meeting and the detection of anti-microbial resistance genes in (scientific) samples the place a number of plasmids could be current.

Antibiotic resistance and plasmid evaluation of Enterobacteriaceae remoted from retail meat in Lagos Nigeria

 

Background: The presence of antibiotic resistant microorganisms in meals is of nice concern globally. This analysis was carried out to detect and characterize plasmid carriage and profiles amongst members of Enterobacteriaceae from completely different meat sorts in Nigeria.

Technique: From a complete of 80 meat samples comprising of mutton,pork, beef and rooster, organisms belonging to the household Enterobacteriaceae wereisolated by commonplace procedures and recognized by API 20E system. Antibiotics susceptibilities testing (AST) againstselected courses of antimicrobial brokers and plasmid extraction was carried outby disc diffusion and alkaline lysis strategies respectively.

Outcomes: One-hundred and ten Enterobacteriaceae have been remoted,species identification revealed isolates belonging to 7 genera comprising of Escherichia, Enterobacter, Klebsiella,Citrobacter, Proteus, Salmonella and Serratia. Total resistance of theorganisms to amoxycillin/clavulanic acid was 91 (82.7%), streptomycin 85(75.7%) and perfloxacin 74 (67.2%) whereas ofloxacin had the highestsusceptibility fee (91.8%). Plasmids profiling revealed ranges of plasmids from1 to three copies with estimated sizes vary of 700bp to 1.1kb amongst E. coli, Okay. pneumoniae, E. aerogenesand Proteus mirabilis. All theisolates with plasmids have been multidrug resistant and have been remoted from rooster excepta pressure of E. coli from pork whichharboured a single plasmid copy suggesting these meat as reservoirs forantibiotic resistant micro organism.

Conclusion: Our findings revealed excessive stage of meat contamination with antibioticresistant Enterobacteriaceae harbouring resistant plasmids. An integratedsurveillance system and security follow should be ensured among the many processorsand retailers.

Identification of a novel plasmid-mediated carbapenemase-encoding gene blaVMB-2 in Vibrio diabolicus

 

We characterised a carbapenem-resistant Vibrio diabolicus pressure of shrimp origin with numerous experiments and bioinformatics evaluation. A novel metallo-β-lactamase gene blaVMB-2, conferring resistance to β-lactams together with meropenem and cephalosporins, was recognized on a plasmid-borne composite transposon ISShfr9-ISCR1blaVMB-2blaCARB-12aadA1-ISShfr9 able to producing blaVMB-2-bearing round intermediate. ISShfr9 was discovered disseminated on MDR pathogens, arousing the priority of additional transmission of blaVMB-2-bearing round intermediate to scientific Enterobacterales through such insertion sequence, which warrants additional investigations.

Outcomes of Dynamic Distinction-Enhanced Ultrasound Correlate With Therapy Final result in Canine Neoplasia Handled With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer

 

Electrochemotherapy (ECT) and/or gene electrotransfer of plasmid DNA encoding interleukin-12 (GET pIL-12) are efficient remedies for canine cutaneous, subcutaneous, and maxillofacial tumors. Regardless of the scientific efficacy of the mixed remedies of ECT and GET, knowledge on parameters which may predict the result of the remedies are nonetheless missing. This research aimed to research whether or not dynamic contrast-enhanced ultrasound (DCE-US) outcomes of subcutaneous tumors differ between tumors with full response (CR) and tumors with out full response (non-CR) in canine handled with ECT and GET pIL-12. Eight canine with a complete of 12 tumor nodules handled with ECT and GET pIL-12 have been included. DCE-US examinations have been carried out in all animals earlier than and instantly after remedy in addition to Eight h and 1, 3, and seven days later.

Medical follow-up examinations have been carried out 7 and 14 days, 1 and 6 months, and 1 yr after therapy. Quite a few vital variations in DCE-US parameters have been famous between tumors with CR and non-CR tumors; perfusion and perfusion heterogeneity have been decrease in CR tumors than in non-CR tumors. Due to this fact, research with bigger numbers of sufferers are wanted to research whether or not DCE-US outcomes can be utilized to foretell therapy outcomes and to make efficient choices in regards to the want for repeated remedy or completely different therapy mixtures in particular person sufferers.

heraeus-targets

heraeus-targets

Excessive-throughput analysis of polymeric nanoparticles for tissue-targeted gene expression utilizing barcoded plasmid DNA

More and more, analysis laboratories are fabricating libraries of novel nanoparticles, engineering each new biomaterial constructions and composition ratios of multicomponent methods. But, strategies for screening gene supply automobiles straight in vivo are typically low-throughout, limiting the variety of candidate nanoparticles that may be investigated. Right here, we report a complete, high-throughput technique to judge a library of polymeric nanoparticles in vivo for tissue-specific gene supply. The tactic entails pairing every nanoparticle formulation with a plasmid DNA (pDNA) that harbors a novel nucleotide sequence serving because the figuring out “barcode”. Utilizing actual time quantitative PCR (qPCR) for detection of the barcoded pDNA and quantitative reverse transcription PCR (RT-qPCR) for transcribed barcoded mRNA, we are able to quantify accumulation and transfection in tissues of curiosity. The barcode pDNA and primers have been designed with adequate sensitivity and specificity to judge a number of nanoparticle formulations per mouse, enhancing screening effectivity.

Utilizing this platform, we evaluated the biodistribution and transfection of Eight intravenously administered poly(beta-amino ester) (PBAE) nanoparticle formulations, every with a PBAE polymer of differential construction. Important ranges of nanoparticle accumulation and gene transfection have been noticed primarily in organs concerned in clearance, together with spleen, liver, and kidneys. Curiously, increased ranges of transfection of choose organs didn’t essentially correlate with increased ranges of tissue accumulation, highlighting the significance of straight measuring in vivo transfection effectivity as the important thing barcoded parameter in gene supply vector optimization. To validate this technique, nanoparticle formulations have been used individually for luciferase pDNA supply in vivo. The distribution of luciferase expression in tissues matched the transfection evaluation by the barcode qPCR technique, confirming that this platform can be utilized to precisely consider systemic gene supply.

 

Prigrow II Medium

TM002 500ml
EUR 125

Prigrow IV Medium

TM004 500ml
EUR 125

Prigrow XI Medium

TM011 450ml
EUR 125

Prigrow V Medium

TM015 500ml
EUR 145

Prigrow VI Medium

TM016 500ml
EUR 145

Prigrow IX Medium

TM019 500ml
EUR 145

Prigrow XV Medium

TM027 500 ml
EUR 145

Prigrow CI Medium

TM101 500ml
EUR 145

Prigrow X.1 Medium

TM010 1 Kit
EUR 385

Prigrow XII Medium

TM012 500ml
EUR 125

Prigrow XIV Medium

TM014 500 ml
EUR 195

Prigrow VII Medium

TM017 500ml
EUR 145

Prigrow XIII Medium

TM013 500 ml
EUR 125

Prigrow VIII Medium

TM018 500ml
EUR 145

Prigrow XVIII Medium

TM039 500 ml
EUR 195

EXS III Basal Medium

30630164-1 25 L
EUR 23.69

EXS III Basal Medium

30630164-2 50 L
EUR 43

Artificial Seawater Nutrient Medium (III)

PT153-10X1L 1 unit
EUR 12.95
Description: Artificial Seawater Nutrient Medium (III)

Artificial Seawater Nutrient Medium (III)

PT153-25L 1 unit
EUR 25.14
Description: Artificial Seawater Nutrient Medium (III)

Artificial Seawater Nutrient Medium (III)

PT153-5L 1 unit
EUR 5.63
Description: Artificial Seawater Nutrient Medium (III)

Hosta Rooting Medium Stage III

30630167-3 25 L
EUR 61.84

Hosta Rooting Medium Stage III

30630167-4 50 L
EUR 116.82

Gentodenz

19-DENZ-500 500 g
EUR 416

EXS III Basal Salt Medium, w/ Adenine

30630163-1 25 L
EUR 21.41

EXS III Basal Salt Medium, w/ Adenine

30630163-2 50 L
EUR 36.33

KATO III [KATO 3] Cells Complete Medium

CM-0372-125mL4 125 mL×4
EUR 108
Description: Complete Growth Medium

KATO III [KATO 3] Cells Complete Medium

CM-0372 125mL×4
EUR 108
  • This product can be stored at 2-8°C for 3 months with shading light.
Description: Cell lines complete growth medium

KATO III [KATO 3] Cells Complete Medium

MBS2568801-4x125mL 4x125mL
EUR 160

Rye Agar A

765-M1854-500G 500 g
EUR 82

Rye Agar B

765-M1855-500G 500 g
EUR 93

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

QuantiChrom Hemoglobin Assay Kit

65-DIHB-250 250 tests
EUR 473

Malachite Green Phosphate Assay kit

65-POMG-25H 2500 tests
EUR 333

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 85.2

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 579.6
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • Ask for price
  • Ask for price
  • 1L
  • 500mL


  • Working procedure:
    1) Invert the bottle a few times to ensure the solution is properly suspended.
    2) Take the gel out of the gel tank and submerse the gel in enough SDS-Blue to cover the gel.
    3) Bands will be visi
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Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 462
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Mouse Anti TNP Immunotoxicity

198-TNPG-1 100 µL
EUR 469.2

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
EUR 469.2

1-Step Polymorphs, Human Cell Separation

71-AN221725 1
EUR 238.8

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
  • The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes
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Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
  • The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes
  • Show more
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

GMP Recombinant Human Interleukin-4 (IL-4)

GMPhuIL-4-50ug 50ug
EUR 528

Pringsheim's Medium

M698-100G 1 unit
EUR 23.3
Description: Pringsheim's Medium

BAT Medium (Alicyclobacillus Medium)

M1561-500G 1 unit
EUR 43.27
Description: BAT Medium (Alicyclobacillus Medium)

Cooked M Medium (R.C. Medium)

M149-100G 1 unit
EUR 27.32
Description: Cooked M Medium (R.C. Medium)

Cooked M Medium (R.C. Medium)

M149-500G 1 unit
EUR 130.49
Description: Cooked M Medium (R.C. Medium)

MSC Prime Medium, 25x

TBS8022-01 1 mL
EUR 76

MSC Prime Medium, 25x

TBS8022-05 5 mL
EUR 268

MSC Prime Medium, 25x

TBS8022-25 25 mL
EUR 968

Lowenstein - Jensen Medium (L.J. Medium)

MM162-100G 1 unit
EUR 12.1
Description: Lowenstein - Jensen Medium (L.J. Medium)

Lowenstein - Jensen Medium (L.J. Medium)

MM162-500G 1 unit
EUR 34.85
Description: Lowenstein - Jensen Medium (L.J. Medium)

ATCC Medium 1754: PETC Medium (Tetra Pac

M2104-500G 1 unit
EUR 63.72
Description: ATCC Medium 1754: PETC Medium (Tetra Pac

General Freeze Medium

PB180436-10mL10 10 mL×10
EUR 158
Description: Supplements & Reagents

General Freeze Medium

PB180436-10mL5 10 mL×5
EUR 98
Description: Supplements & Reagents

General Freeze Medium

MBS2567647-10x10mL 10x10mL
EUR 195

General Freeze Medium

MBS2567647-5x10mL 5x10mL
EUR 155

Human iPS Growth Medium (PSGro Medium)

TBS8023 500 mL
EUR 189

Rat Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-25023 5 x 100 ml Ask for price

General Freezing Medium

PB180436 10mL×5
EUR 98
  • This product can be stored at 2-8°C for 3 months or -20°C for 24 months.
Description: Others

Medium 199

C0012-01 RT 500 mL Bottle
EUR 34

Medium 199

MBS2557648-10L 10L
EUR 165

Medium 199

MBS2557648-50L 50L
EUR 345

Medium 199

MBS2557648-5L 5L
EUR 150

Medium 199

MBS2557648-5x50L 5x50L
EUR 1060

Human Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-46043 5 x 100 ml Ask for price

HS Medium (Hydrosulphite of Sodium Medium)

77707 100 Gms
EUR 7.7
  • 38210000
Description: Part D

HS Medium (Hydrosulphite of Sodium Medium)

77707-1 500 Gms
EUR 25.66
  • 38210000
Description: Part D

SIM Medium (Sulfite Indole Motility Medium)

49810 100 Gms
EUR 6.84
  • 38210000
Description: Part D

SIM Medium (Sulfite Indole Motility Medium)

49810-1 500 Gms
EUR 27.37
  • 38210000
Description: Part D

Listeria Enrichment Medium Base (UVM Medium)

28685 100 Gms
EUR 11.97
  • 38210000
Description: Part D

Listeria Enrichment Medium Base (UVM Medium)

28685-1 500 Gms
EUR 54.74
  • 38210000
Description: Part D

MlO Medium (Motility Indole Ornithine Medium)

88736 100 Gms
EUR 7.7
  • 38210000
Description: Part D

MlO Medium (Motility Indole Ornithine Medium)

88736-1 500 Gms
EUR 31.64
  • 38210000
Description: Part D

Phyto Proteose Agar Medium Base 
(KB Medium)

PHM008-100G 1 unit
EUR 13.81
Description: Phyto Proteose Agar Medium Base 
(KB Medium)

Phyto Proteose Agar Medium Base 
(KB Medium)

PHM008-500G 1 unit
EUR 60.56
Description: Phyto Proteose Agar Medium Base 
(KB Medium)

MIU Medium Base (Motility Indole Urea Medium)

16309 100 Gms
EUR 9.41
  • 38210000
Description: Part D

MIU Medium Base (Motility Indole Urea Medium)

16309-1 500 Gms
EUR 35.07
  • 38210000
Description: Part D

EC MEDIUM

E05-100-10kg 10 kg
EUR 976.8

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 259.2

EC MEDIUM

E05-100-500g 500 g
EUR 114

DC MEDIUM

D04-117-10kg 10 kg
EUR 1894.8

DC MEDIUM

D04-117-2kg 2kg
EUR 458.4

DC MEDIUM

D04-117-500g 500 g
EUR 168

A Medium

DJ1018 100g
EUR 101.76
  • Product category: Culture Media/Medium

VP Medium

M662-500G 1 unit
EUR 52.25
Description: VP Medium

E.T. Medium

M854-500G 1 unit
EUR 36.34
Description: E.T. Medium

E.T. Medium

M854-5KG 1 unit
EUR 355.04
Description: E.T. Medium
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heraeus-targets

Revealing photon transmission in an ultraviolet reactor: Advanced approaches for measuring fluence rate distribution in water for model validationRevealing photon transmission in an ultraviolet reactor: Advanced approaches for measuring fluence rate distribution in water for model validation

Fluence price (FR) distribution (optical discipline) is of nice significance within the optimum design of ultraviolet (UV) reactors for disinfection or oxidation processes in water remedy. Because the 1970s, varied