Antimicrobial resistance related to the unfold of plasmid-encoded extended-spectrum β-lactamase (ESBL) genes conferring resistance to 3rd technology cephalosporins is growing worldwide. Nonetheless, knowledge on the inhabitants of ESBL producing E. coli in several animal sources and their antimicrobial traits are restricted. The aim of this research was to research potential reservoirs of ESBL-encoded genes in E. coli remoted from swine, beef, dairy, and poultry collected from completely different areas of the USA utilizing whole-genome sequencing (WGS).
300 isolates had been typed into completely different phylogroups, characterised by BOX AIR-1 PCR and examined for resistance to antimicrobials. Of the 300 isolates, 59.7% had been immune to sulfisoxazole, 49.3% to tetracycline, 32.3% to cephalothin, 22.3% to ampicillin, 20% to streptomycin, 16% to ticarcillin; resistance to the remaining 12 antimicrobials was lower than 10%. Phylogroups A and B1 had been most prevalent with A (n = 92, 30%) and B1 (87 = 29%). A complete of 9 E. coli isolates had been confirmed as ESBL producers by double-disk synergy testing and multidrug resistant (MDR) to at the least three antimicrobial drug lessons. Utilizing WGS, considerably increased numbers of ESBL-E. coli had been detected in swine and dairy manure than from another animal sources, suggesting that these would be the main animal sources for ESBL producing E. coli. These isolates carry plasmids, reminiscent of IncFIA(B), IncFII, IncX1, IncX4, IncQ1, CollRNAI, Col440I, and purchased ARGs aph(6)-Id, aph(3″)-Ib, aadA5, aph(3′)-Ia, blaCTX-M-15, blaTEM-1B, mphA, ermB, catA1, sulsultetB, dfrA17. One of many E. coli isolates from swine with ST 410 was immune to 9 antibiotics and carried greater than 28 virulence elements, and this ST has been proven to belong to a global high-risk clone. Our knowledge means that ESBL producing E. coli are broadly distributed in several animal sources, however swine and dairy cattle could also be their most important reservoir.
Recombinant Antibody Manufacturing Utilizing a Twin-Promoter Single Plasmid System
Monoclonal antibodies (mAbs) have demonstrated large results on the remedy of assorted illness indications and stay the quickest rising class of therapeutics. Manufacturing of recombinant antibodies is carried out utilizing mammalian expression methods to facilitate native antibody folding and post-translational modifications. Typically, mAb expression methods make the most of co-transfection of heavy chain (hc) and light-weight chain (lc) genes encoded on separate plasmids. On this research, we look at the manufacturing of two FDA-approved antibodies utilizing a bidirectional (BiDi) vector encoding each hc and lc with mirrored promoter and enhancer components on a single plasmid, by analysing the person hc and lc mRNA expression ranges and subsequent quantification of fully-folded IgGs on the protein stage.
From the evaluation of various promoter combos, we’ve developed a generic expression vector comprised of mirrored enhanced CMV (eCMV) promoters exhibiting comparable mAb yields to a two-plasmid reference. This research paves the best way to facilitate small-scale mAb manufacturing by transient cell transfection with a single vector in a cost- and time-efficient method.
Genomic evaluation and phylogenetic place of the complicated IncC plasmid discovered within the Spanish monophasic clone of Salmonella enterica serovar Typhimurium
pUO-STmRV1 is an IncC plasmid found within the Spanish clone of the emergent monophasic variant of Salmonella enterica serovar Typhimurium, which has most likely contributed to its epidemiological success. The sequence of the whole plasmid decided herein revealed a largely degenerated spine with accent DNA integrated at 4 completely different places. The acquired DNA constitutes greater than two-thirds of the pUO-STmRV1 genome and originates from plasmids of various incompatibility teams, together with IncF (reminiscent of R100 and pSLT, the virulence plasmid particular of S. Typhimurium), IncN and IncI, from the integrative aspect GIsul2, or from but unknown sources. Along with pSLT virulence genes, the plasmid carries genes conferring resistance to widely-used antibiotics and heavy metals, along with a wealth of genetic components concerned in DNA mobility.
The latter comprise class 1 integrons, transposons, pseudo-transposons, and insertion sequences, strikingly with 14 copies of IS26, which may have performed a vital function within the meeting of the complicated plasmid. Typing of pUO-STmRV1 revealed spine options characteristically related to sort 1 and kind 2 IncC plasmids and will due to this fact be thought to be a hybrid plasmid. Nonetheless, a rooted phylogenetic tree based mostly on core genes signifies that it somewhat belongs to an historical lineage which diverged at an early stage from the department resulting in most extant IncC plasmids detected thus far. pUO-STmRV1 might have developed at a time when uncontrolled use of antibiotics and biocides favored the buildup of a number of resistance genes inside an IncC spine. The ensuing plasmid thus allowed the Spanish clone to face up to all kinds of hostile circumstances, whereas concurrently selling its personal propagation by means of vertical transmission.
Plasmid-Free System and Modular Design for Environment friendly 5-Aminolevulinic Acid Manufacturing by Engineered Escherichia coli
5-Aminolevulinic acid (ALA) is a vital intermediate for a lot of organisms and has been thought-about for the functions of medical particularly in photodynamic remedy of most cancers not too long ago. Nonetheless, ALA manufacturing through chemical strategy is difficult; therefore, microbial manufacturing has acquired extra attentions. On this research, a modular design to concurrently specific ALA synthase from Rhodobacter sphaeroides (RshemA), a non-specific ALA exporter (RhtA), and chaperones was first developed and mentioned. The ALA manufacturing was considerably elevated by coexpressing RhtA and RshemA.
Moreover, ALA was enhanced by the cofactor pyridoxal phosphate (PLP) which was provided by expressing genes of pdxK and pdxY or direct addition. Nonetheless, inclusion our bodies of RshemA served as an impediment; thus, chaperones DnaK and GroELS had been launched to reform the conformation of proteins and efficiently improved ALA manufacturing. Lastly, a plasmid-free pressure RrGI, because the strong chassis, was established and a 6.23-fold enhancement on ALA biosynthesis and led to 7.47 g/L titer and 0.588 g/L/h productiveness beneath the optimum cultural situation.
heraeus-targets
Uptake and replication in Acanthamoeba castellanii of a virulent (pVAPA-positive) pressure of Rhodococcus equi and its isogenic, plasmid-cured pressure
Rhodococcus equi is a soil saprophytic bacterium and intracellular pathogen that causes pneumonia in foals. Strains of R. equi which are virulent in foals include a plasmid that encodes a virulence-associated protein A (VapA) vital for replication in macrophages. As a result of different intracellular pathogens survive and replicate inside amoebae, we postulated that the VapA-bearing plasmid (pVAPA) confers a survival benefit for R. equi towards environmental predators like amoebae.
To check this speculation, we in contrast phagocytosis by and survival in Acanthamoeba castellanii of isogenic strains of pVAPA-positive and pVAPA-negative R. equi. Phagocytosis of the pVAPA-negative pressure by A. castellanii was considerably (P < 0.0001) larger than the pVAPA-positive pressure. Intracellular replication of the pVAPA-positive pressure in A. castellanii was considerably (P < 0.0001) larger than the pVAPA-negative pressure throughout each 48 h and 9 days. These outcomes point out that the presence of the VapA plasmid reduces uptake and aids replication of R. equi in A. castellanii.
 Gamborg B5 Medium, w/ Vitamins |
| 30630029-4 | Glycomatrix | 50 L | EUR 39.53 |
Gamborg B5 Medium, w/ Vitamins |
| 30630029-5 | Glycomatrix | 100 L | EUR 78.08 |
 Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar |
| PT127-10X1L | EWC Diagnostics | 1 unit | EUR 11.59 |
Description: Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar |
Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar |
| PT127-25L | EWC Diagnostics | 1 unit | EUR 15.69 |
Description: Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar |
Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar |
| PT127-5L | EWC Diagnostics | 1 unit | EUR 5.04 |
Description: Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar |
 Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ |
| PT017G-10X1L | EWC Diagnostics | 1 unit | EUR 22.37 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM |
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ |
| PT017G-25L | EWC Diagnostics | 1 unit | EUR 35.75 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM |
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ |
| PT017G-5L | EWC Diagnostics | 1 unit | EUR 9.73 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM |
 Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam |
| PT016-100X1L | EWC Diagnostics | 1 unit | EUR 85.44 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose |
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam |
| PT016-10X1L | EWC Diagnostics | 1 unit | EUR 10.65 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose |
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam |
| PT016-25L | EWC Diagnostics | 1 unit | EUR 21.81 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose |
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam |
| PT016-5L | EWC Diagnostics | 1 unit | EUR 6.39 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose |
 Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, |
| PT017-10X1L | EWC Diagnostics | 1 unit | EUR 17.94 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar |
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, |
| PT017-25L | EWC Diagnostics | 1 unit | EUR 28.74 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar |
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, |
| PT017-5L | EWC Diagnostics | 1 unit | EUR 7.8 |
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar |
 Medium, w/ Gamborg B5 Vitamins) Murashige and Skoog (MS) Medium, w/ Gamborg B5 Vitamins |
| 30630059-2 | Glycomatrix | 25 L | EUR 22.1 |
Murashige and Skoog (MS) Medium, w/ Gamborg B5 Vitamins |
| 30630059-3 | Glycomatrix | 50 L | EUR 43.43 |
 Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins |
| PT114-10X1L | EWC Diagnostics | 1 unit | EUR 15.85 |
Description: Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins & Sucrose;w/o Agar |
Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins |
| PT114-25L | EWC Diagnostics | 1 unit | EUR 23.84 |
Description: Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins & Sucrose;w/o Agar |
Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins |
| PT114-5L | EWC Diagnostics | 1 unit | EUR 6.89 |
Description: Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins & Sucrose;w/o Agar |
 Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin |
| PT025-100X1L | EWC Diagnostics | 1 unit | EUR 80.14 |
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins |
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin |
| PT025-10X1L | EWC Diagnostics | 1 unit | EUR 10.05 |
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins |
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin |
| PT025-25L | EWC Diagnostics | 1 unit | EUR 17.85 |
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins |
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin |
| PT025-5L | EWC Diagnostics | 1 unit | EUR 5.95 |
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins |
 Gamborg's B-5 Medium; With Vitamins |
| CP011-010 | GenDepot | 10X1L | EUR 121.2 |
Gamborg's B-5 Medium; With Vitamins |
| CP011-500 | GenDepot | 50L | EUR 151.2 |
) Gamborg B5 Vitamins Solution (1000X) |
| PL018-1X50ML | EWC Diagnostics | 1 unit | EUR 16.44 |
Description: Gamborg B5 Vitamins Solution (1000X) |
Gamborg B5 Vitamins Solution (1000X) |
| PL018-5X50ML | EWC Diagnostics | 1 unit | EUR 70.53 |
Description: Gamborg B5 Vitamins Solution (1000X) |
 Gamborg's B-5 Medium; With Vitamins and Sucrose |
| CP012-010 | GenDepot | 10X1L | EUR 124.8 |
Gamborg's B-5 Medium; With Vitamins and Sucrose |
| CP012-500 | GenDepot | 50L | EUR 151.2 |
 Gamborg B5 Medium |
| 30630028-3 | Glycomatrix | 50 L | EUR 36.33 |
 NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT094-25L | EWC Diagnostics | 1 unit | EUR 21.74 |
Description: NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT094-5L | EWC Diagnostics | 1 unit | EUR 5.78 |
Description: NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Gamborg B5 Vitamin Mix |
| 30630027-1 | Glycomatrix | 100 mL | EUR 32.46 |
 Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin |
| PT042-10X1L | EWC Diagnostics | 1 unit | EUR 11.59 |
Description: Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin |
| PT042-25L | EWC Diagnostics | 1 unit | EUR 18.5 |
Description: Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin |
| PT042-5L | EWC Diagnostics | 1 unit | EUR 5.04 |
Description: Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin |
| PT143-25L | EWC Diagnostics | 1 unit | EUR 20.46 |
Description: Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin |
| PT143-5L | EWC Diagnostics | 1 unit | EUR 5.37 |
Description: Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ |
| PT032-10X1L | EWC Diagnostics | 1 unit | EUR 11.59 |
Description: DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ |
| PT032-25L | EWC Diagnostics | 1 unit | EUR 15.09 |
Description: DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ |
| PT032-5L | EWC Diagnostics | 1 unit | EUR 5.04 |
Description: DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/) Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ |
| PT033-10X1L | EWC Diagnostics | 1 unit | EUR 10.62 |
Description: Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ |
| PT033-25L | EWC Diagnostics | 1 unit | EUR 13.85 |
Description: Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ |
| PT033-5L | EWC Diagnostics | 1 unit | EUR 4.61 |
Description: Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT018-100X1L | EWC Diagnostics | 1 unit | EUR 72.01 |
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT018-10X1L | EWC Diagnostics | 1 unit | EUR 12.35 |
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT018-25L | EWC Diagnostics | 1 unit | EUR 18.27 |
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT018-5L | EWC Diagnostics | 1 unit | EUR 5.37 |
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT040-10X1L | EWC Diagnostics | 1 unit | EUR 11.48 |
Description: Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT040-25L | EWC Diagnostics | 1 unit | EUR 15.28 |
Description: Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT040-5L | EWC Diagnostics | 1 unit | EUR 4.99 |
Description: Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins |
| PT056-10X1L | EWC Diagnostics | 1 unit | EUR 11.59 |
Description: Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins |
| PT056-25L | EWC Diagnostics | 1 unit | EUR 18.53 |
Description: Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins |
| PT056-5L | EWC Diagnostics | 1 unit | EUR 5.04 |
Description: Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V |
| PT057-10X1L | EWC Diagnostics | 1 unit | EUR 11.59 |
Description: Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V |
| PT057-25L | EWC Diagnostics | 1 unit | EUR 18.5 |
Description: Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V |
| PT057-5L | EWC Diagnostics | 1 unit | EUR 5.04 |
Description: Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V |
| PT086-10X1L | EWC Diagnostics | 1 unit | EUR 11.59 |
Description: Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V |
| PT086-25L | EWC Diagnostics | 1 unit | EUR 18.41 |
Description: Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V |
| PT086-5L | EWC Diagnostics | 1 unit | EUR 5.04 |
Description: Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V |
| PT139-25L | EWC Diagnostics | 1 unit | EUR 23.26 |
Description: Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V |
| PT139-5L | EWC Diagnostics | 1 unit | EUR 6.1 |
Description: Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT031-10X1L | EWC Diagnostics | 1 unit | EUR 11.59 |
Description: De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT031-25L | EWC Diagnostics | 1 unit | EUR 15.09 |
Description: De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; |
| PT031-5L | EWC Diagnostics | 1 unit | EUR 5.04 |
Description: De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg |
| PT107-10X1L | EWC Diagnostics | 1 unit | EUR 16.05 |
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar |
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg |
| PT107-25L | EWC Diagnostics | 1 unit | EUR 23.93 |
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar |
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg |
| PT107-5L | EWC Diagnostics | 1 unit | EUR 6.98 |
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar |
 Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ |
| PT012-10X1L | EWC Diagnostics | 1 unit | EUR 13.67 |
Description: Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose |
Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ |
| PT012-25L | EWC Diagnostics | 1 unit | EUR 20.46 |
Description: Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose |
Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ |
| PT012-5L | EWC Diagnostics | 1 unit | EUR 5.95 |
Description: Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose |
 Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ |
| PT013-10X1L | EWC Diagnostics | 1 unit | EUR 13.32 |
Description: Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose |
Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ |
| PT013-25L | EWC Diagnostics | 1 unit | EUR 19.97 |
Description: Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose |
Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ |
| PT013-5L | EWC Diagnostics | 1 unit | EUR 5.78 |
Description: Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose |
 NLN Medium, w/ Vitamins |
| 30630103-2 | Glycomatrix | 25 L | EUR 21.06 |
NLN Medium, w/ Vitamins |
| 30630103-3 | Glycomatrix | 50 L | EUR 43.43 |
 Medium w/ Vitamins; w/ Vitamins; w/ Vita) Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vita |
| PT035-10X1L | EWC Diagnostics | 1 unit | EUR 11.59 |
Description: Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vita |
| PT035-25L | EWC Diagnostics | 1 unit | EUR 14.96 |
Description: Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vita |
| PT035-5L | EWC Diagnostics | 1 unit | EUR 5.04 |
Description: Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins |
 Litvay Medium, w/ Vitamins |
| 30630049-3 | Glycomatrix | 25 L | EUR 32.37 |
Litvay Medium, w/ Vitamins |
| 30630049-4 | Glycomatrix | 50 L | EUR 43.46 |
 White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ V |
| PT014-10X1L | EWC Diagnostics | 1 unit | EUR 13.67 |
Description: White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose |
White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ V |
| PT014-25L | EWC Diagnostics | 1 unit | EUR 20.46 |
Description: White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose |
