Conditional reprogramming: subsequent technology cell culture

heraeus-targets

Prolonged-term main custom of mammalian cells has been always robust attributable to unavoidable senescence. Commonplace methods for producing immortalized cell strains usually require manipulation of genome which leads to change of significant natural and genetic traits. Not too way back, conditional reprogramming (CR) emerges as a novel subsequent know-how instrument for long-term custom of main epithelium cells derived from almost all origins with out alteration of genetic background of main cells.

CR co-cultures main cells with inactivated mouse 3T3-J2 fibroblasts inside the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling main cells to build up stem-like traits whereas retain their potential to utterly differentiate.

With just some years’ progress, CR displays broad prospects in functions in diversified areas along with sickness modeling, regenerative medicine, drug evaluation, drug discovery along with precision medicine. This consider is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its broad functions, highlighting the price of CR in every main and translational researches and discussing the challenges confronted with CR.

Outcomes of Kifunensine on Manufacturing and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture Bioreactor

The manufacturing and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a model extraordinarily glycosylated therapeutic protein, in a transgenic rice cell suspension custom dealt with with kifunensine, a robust α-mannosidase I inhibitor, was studied in a 5 L bioreactor.

A media commerce was carried out at day 7 of cultivation by eradicating spent sugar-rich medium (NB+S) and together with latest sugar-free (NB-S) medium to induce the rice α-amylase 3D (RAmy3D) promoter to offer rice recombinant human BChE (rrBChE). Using a 1.25X-concentrated sugar-free medium together with an 80% lowered working amount via the media commerce led to a whole energetic rrBChE manufacturing diploma of 79 ± 2 µg (g FW)-1 or 7.5 ± 0.4 mg L-1 inside the presence of kifunensine, which was 1.5-times higher than our earlier bioreactor runs using common sugar-free (NB-S) media with no kifunensine treatment.

Importantly, the amount of secreted energetic rrBChE in custom medium was enhanced inside the presence of kifunensine, comprising 44% of all the energetic rrBChE at day 5 following induction. Coomassie-stained SDS-PAGE gel and Western blot analyses revealed utterly completely different electrophoretic migration of purified rrBChE bands with and with out kifunensine treatment, which was attributed to utterly completely different N-glycoforms. N-Glycosylation analysis confirmed significantly elevated oligomannose glycans (Man5/6/7/8) in rrBChE dealt with with kifunensine compared with controls. Nonetheless, the mass-transfer limitation of kifunensine was seemingly the principle objective for incomplete inhibition of α-mannosidase I on this bioreactor look at.

 

Variations in drug sensitivity between two-dimensional and three-dimensional custom strategies in triple-negative breast most cancers cell strains

Three-dimensional (3D) custom shows tumor biology complexities in distinction with two-dimensional (2D) custom. Thus, 3D custom has attracted consideration in cell biology analysis along with drug sensitivity exams.

Herein, we investigated variations in anticancer drug sensitivities between 2D and 3D custom strategies in triple-negative breast most cancers (TNBC) cell strains. 13 TNBC cell strains have been maintained in 2D and 3D cultures for Three days sooner than drug publicity. Cell morphology inside the 3D custom was examined by phase-contrast microscopy.

Sensitivities to epirubicin (EPI), cisplatin (CDDP), and docetaxel (DTX) have been investigated by cell viability assay in every cultures and in distinction. The IC50s of all Three remedy have been significantly higher inside the 3D custom than inside the 2D custom in most cell strains.

These have been correlated between the 2D and 3D cultures in EPI (R = 0.555) and CDDP (R = 0.955), nonetheless not in DTX (R = 0.221). Spherical spheroid-forming cells have been additional proof in opposition to brokers than grape-like kinds. In conclusion, 3D custom was additional proof in opposition to all Three remedy than 2D custom in most TNBC cell strains. Sensitivity to CDDP was extraordinarily correlated between the 2D and 3D cultures, nonetheless to not DTX. 2D custom is also acceptable for sensitivity check out for DNA-damaging brokers.

 

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Growth of a 3D thoughts extracellular matrix model to overview the interaction between microglia and T cells in co-custom

Neurodegenerative points are characterised by the activation of brain-resident microglia cells and by the infiltration of peripheral T cells. Nonetheless, their interplay in sickness has not been clarified however. It is robust to analysis difficult cellular dynamics in dwelling animals, and simple two-dimensional (2D) cell custom fashions do not resemble the fragile 3D building of thoughts tissue. Subsequently, we developed a biomimetic 3D in vitro custom system for co-cultivation of microglia and T cells.

Given that activation and/or migration of immune cells inside the thoughts is maybe affected by components of the extracellular matrix, outlined 3D fibrillar collagen I-based matrices have been constructed and modified with hyaluronan and/or chondroitin sulphate, resembling factors of thoughts extracellular matrix. Murine microglia and spleen-derived T cells have been cultured alone or in co-culture on the constructed matrices. Microglia exhibited in vivo-like morphology and T cells confirmed enhanced survival when co-cultured with microglia or to a minor diploma inside the presence of glia-conditioned medium.

The open and porous fibrillar building of the matrix allowed for cell invasion and direct cell-cell interaction, with stronger invasion of T cells. Every cell kinds confirmed no dependence on the matrix modifications. Microglia could be activated on the matrices by lipopolysaccharide resulting in interleukin-6 and tumor necrosis factor-α secretion. The findings herein level out that biomimetic 3D matrices allow for co-cultivation and activation of main microglia and T cells and provide useful devices to overview their interaction in vitro.

 

A two-dimensional multiwell cellculture technique for the manufacturing of CYP3A4-expressing hepatocyte-like cells from HepaRG cells

Cytochrome P450 enzymes (CYP) function in drug metabolism inside the liver. To guage fairly just a few drug candidates, a high-content screening (HCS) system with hepatocyte-like cells (HLCs) that will change grownup human hepatocytes is required. Human hepatocellular carcinoma HepaRG is the one cell line capable of providing HLCs with extreme CYP3A4 expression akin to that in grownup hepatocytes after cell differentiation.

The aim of this look at was to design an excellent multiwell custom system for HLCs using transgenic HepaRG cells expressing the EGFP coding an enhanced inexperienced fluorescent protein beneath CYP3A4 transcriptional regulation. HLCs have been matured on 5 a number of varieties of 96-well black plates.

Culturing HLCs on glass-bottom Optical CVG plates significantly promoted cell maturation and elevated metabolic train by twofold beneath two-dimensional (2D) custom conditions, and these choices have been enhanced by 2% collagen coating.

Three plates for three-dimensional (3D) cell cultures with a gas-exchangeable material or dimethylpolysiloxane membrane bottom formed numerous spherical colonies, whereas they’ve been ineffective for CYP3A4 expression. Beneath optimized conditions provided proper right here,HLCs misplaced responsiveness to nuclear receptor-mediated transcriptional induction of CYP3A4, suggesting that CYP3A4 transcription has already been completely upregulated.

Subsequently, HepaRG-derived HLCs will current another option to human hepatocytes with extreme ranges of CYP3A4 enzyme train even beneath 2D custom conditions. This could improve numerous drug screening methods.

Gamborg B5 Medium, w/ Vitamins
30630029-5 100 L
EUR 78.08
Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar
PT127-10X1L 1 unit
EUR 11.59
Description: Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar
Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar
PT127-25L 1 unit
EUR 15.69
Description: Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar
Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar
PT127-5L 1 unit
EUR 5.04
Description: Gamborg B5 Medium;w/ Vitamins;w/o Sucrose & Agar
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/
PT017G-10X1L 1 unit
EUR 22.37
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/
PT017G-25L 1 unit
EUR 35.75
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/
PT017G-5L 1 unit
EUR 9.73
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam
PT016-100X1L 1 unit
EUR 85.44
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam
PT016-10X1L 1 unit
EUR 10.65
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam
PT016-25L 1 unit
EUR 21.81
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam
PT016-5L 1 unit
EUR 6.39
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2,
PT017-10X1L 1 unit
EUR 17.94
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2,
PT017-25L 1 unit
EUR 28.74
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2,
PT017-5L 1 unit
EUR 7.8
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar
MS BASAL MEDIUM w/ GAMBORG VITAMINS
M404 100L
EUR 29.84
Murashige and Skoog (MS) Medium, w/ Gamborg B5 Vitamins
30630059-2 25 L
EUR 22.1
Murashige and Skoog (MS) Medium, w/ Gamborg B5 Vitamins
30630059-3 50 L
EUR 43.43
Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins
PT114-10X1L 1 unit
EUR 15.85
Description: Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins & Sucrose;w/o Agar
Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins
PT114-25L 1 unit
EUR 23.84
Description: Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins & Sucrose;w/o Agar
Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins
PT114-5L 1 unit
EUR 6.89
Description: Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins & Sucrose;w/o Agar
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin
PT025-100X1L 1 unit
EUR 80.14
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin
PT025-10X1L 1 unit
EUR 10.05
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin
PT025-25L 1 unit
EUR 17.85
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin
PT025-5L 1 unit
EUR 5.95
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins
Gamborg's B-5 Medium; With Vitamins
CP011-010 10X1L
EUR 121.2
Gamborg's B-5 Medium; With Vitamins
CP011-500 50L
EUR 151.2
Gamborg B5 Vitamins Solution (1000X)
PL018-1X50ML 1 unit
EUR 16.44
Description: Gamborg B5 Vitamins Solution (1000X)
Gamborg B5 Vitamins Solution (1000X)
PL018-5X50ML 1 unit
EUR 70.53
Description: Gamborg B5 Vitamins Solution (1000X)
Gamborg's B-5 Medium; With Vitamins and Sucrose
CP012-010 10X1L
EUR 124.8
Gamborg's B-5 Medium; With Vitamins and Sucrose
CP012-500 50L
EUR 151.2
Gamborg B5 Medium
30630028-2 25 L
EUR 21.5
Gamborg B5 Medium
30630028-3 50 L
EUR 36.33
NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT094-25L 1 unit
EUR 21.74
Description: NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT094-5L 1 unit
EUR 5.78
Description: NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Gamborg B5 Vitamin Mix
30630027-1 100 mL
EUR 32.46
Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT042-10X1L 1 unit
EUR 11.59
Description: Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT042-25L 1 unit
EUR 18.5
Description: Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT042-5L 1 unit
EUR 5.04
Description: Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT143-25L 1 unit
EUR 20.46
Description: Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT143-5L 1 unit
EUR 5.37
Description: Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT032-10X1L 1 unit
EUR 11.59
Description: DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT032-25L 1 unit
EUR 15.09
Description: DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT032-5L 1 unit
EUR 5.04
Description: DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT033-10X1L 1 unit
EUR 10.62
Description: Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT033-25L 1 unit
EUR 13.85
Description: Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT033-5L 1 unit
EUR 4.61
Description: Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT018-100X1L 1 unit
EUR 72.01
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT018-10X1L 1 unit
EUR 12.35
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT018-25L 1 unit
EUR 18.27
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT018-5L 1 unit
EUR 5.37
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT040-10X1L 1 unit
EUR 11.48
Description: Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT040-25L 1 unit
EUR 15.28
Description: Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT040-5L 1 unit
EUR 4.99
Description: Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins
PT056-10X1L 1 unit
EUR 11.59
Description: Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins
PT056-25L 1 unit
EUR 18.53
Description: Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins
PT056-5L 1 unit
EUR 5.04
Description: Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT057-10X1L 1 unit
EUR 11.59
Description: Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT057-25L 1 unit
EUR 18.5
Description: Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT057-5L 1 unit
EUR 5.04
Description: Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT086-10X1L 1 unit
EUR 11.59
Description: Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT086-25L 1 unit
EUR 18.41
Description: Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT086-5L 1 unit
EUR 5.04
Description: Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT139-25L 1 unit
EUR 23.26
Description: Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT139-5L 1 unit
EUR 6.1
Description: Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT031-10X1L 1 unit
EUR 11.59
Description: De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT031-25L 1 unit
EUR 15.09
Description: De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT031-5L 1 unit
EUR 5.04
Description: De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg
PT107-10X1L 1 unit
EUR 16.05
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg
PT107-25L 1 unit
EUR 23.93
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg
PT107-5L 1 unit
EUR 6.98
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar
Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT012-10X1L 1 unit
EUR 13.67
Description: Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT012-25L 1 unit
EUR 20.46
Description: Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT012-5L 1 unit
EUR 5.95
Description: Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT013-10X1L 1 unit
EUR 13.32
Description: Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT013-25L 1 unit
EUR 19.97
Description: Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT013-5L 1 unit
EUR 5.78
Description: Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
NLN Medium, w/ Vitamins
30630103-2 25 L
EUR 21.06
NLN Medium, w/ Vitamins
30630103-3 50 L
EUR 43.43
DKW MEDIUM w/ VITAMINS
D2470 10L
EUR 33.15
Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vita
PT035-10X1L 1 unit
EUR 11.59
Description: Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vita
PT035-25L 1 unit
EUR 14.96
Description: Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vita
PT035-5L 1 unit
EUR 5.04
Description: Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Gabriel Gonzalez

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heraeus-targets

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Background: We examined the impact of dexamethasone prescribed within the preliminary three postoperative weeks on survival, steroid dependency, and an infection in glioblastoma sufferers. Strategies: On this single-center retrospective cohort evaluation, we