Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future.

Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future.

The Ebolavirus 2014/2015 epidemic has resulted in more than 28,000 cases and 11,323 deaths reported in March 2016. The internal transmission of the Guinea’s strain associated with the epidemic has occurred mainly in six African countries and a International transmission has been reported in four countries. The management of epidemics was limited by the inability to quickly diagnose infected cases. About fifteen countries in Africa should be threatened at risk of epidemics of ebolavirus in the future as a result of climate change and urbanization.

Early detection of cases and reduction of transmission rates is essential for preventing and managing severe future epidemics. We have designed a quick test for eboavirus detection using polymerase recombinase amplification, rapid isothermal amplification technology that can be combined with portable lateral feed detection technology. The rapid dosage developed operates in 30 minutes and was comparable to the Taqman ™ PCR in real time. Our dosage could be suitable for implementation in district and primary health laboratories, because only a heating block and a centrifuge are required for operation. The technique could provide a way for rapid screening of patients and animals for better management of epidemics.

Sperm test at home for epidemiological studies: evaluation of the trak men’s fertility test system in a preconception cohort based on the Internet.

Evaluation of the quality of sperm in population-based epidemiological studies presents logistical and financial challenges due to dependence on the centralized analysis of laboratory sperm. The Trak Men Fertility Testing System is a home test erased and validated by the FDA for sperm concentration and cum volume, with a search use only sperm motility test. We evaluate here the overall use of the Trak system among men participating in an online pregnancy study (Presto), a web study on North American couples planning the pregnancy.us male participants aged 21 with ≤6 months attempt at pregnancy at the time of studying the study. Invited to participate in subsidy cum tests after completing their basic questionnaire. Constant participants received a TRAK engine (drum centrifuge) and two test kits. Participants shared their test results via downloaded smartphones images to online questionnaires. The data were then linked to covariate data from the basic questionnaire. Of the 688 men invited, 373 (54%) provided consent and 271 (73%) completed at least one sperm test result.

Sperm volume distributions, sperm concentration, spermatozoa concentration, the total number of spermatozoa and the total number of WHO spermatozoa of the Global Health Organization (WHO) of the Semen Men’s Organization in the general population. The Global Conviviality Score of the TRK system was 1.4 on a 5-point Likert scale (1 = very easy, 5 = difficult) and 92% of the participants thought they did the test correctly and received a precise result. Finally, men with a higher number of spermatozoa were more likely to report the “comfortable” or “excited” feeling as a result of the tests, while men with a small number of motile spermatozoa were more likely to report The feeling “concerned” or “frustrated”. Overall, 91% of men stated that they want to restore again. The TRK system provides a simple and potential cost-effective way to measure significant sperm parameters and can be useful in population-based epidemiological fertility studies.

Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future.

Removal of sperm bacteria with a low density colloid.

Antibiotics are added to sperm extensions when preparing commercial sperm doses for artificial insemination in accordance with national and international guidelines. However, this addition of antibiotics represents non-therapeutic use and could contribute to the development of antibiotic resistance. It has been shown that colloidal centrifugation reduced the load of bacteria present in the sperm of boar and was able to eliminate all bacteria if they are made directly after the collection of sperm, although some losses of spermatozoa.

The present experience was carried out with a low density colloid to investigate the question of whether it was possible to separate all seminal plasma spermatozoons. Without selection of robust spermatozoa or if it would have a harmful effect on the quality of sperm. The ejaculations of nine boars were extended in the thaw solution of Beltsville without antibiotics and were transported to the laboratory for centrifugation at a single layer (SLC) on the modified porcicoll at a low density (s). Another modification was that a sterile internal tube was included in some of the 50 ml centrifuge tubes to facilitate the harvest of the sperm pellet (M). Aliquots of all samples (control, s and m) were cultured for bacterial quantification and identification using standard microbiological methods.

The quality of the sperm has been evaluated daily. Three of the samples C and M and five of S samples do not contain any bacteria. The average bacterial accounts for the remaining samples (colonial training units / ml) were as follows. Citrobacter spp., Staphylococcus simulans, Klebsiella Variica, Escherichia coli, Myroids Odoratimimus, Proteus spp. and Enterococcus fecalis have been identified in the control samples. There were marginal differences in the quality of sperm in spermatozoa, the speed of sperm and linearity being higher in s samples and m than in c at all times.

However, the viability of spermatozoa, capacity capacity and the status of acrosome have been marginally better in commands than in S or M day 0, but these differences have disappeared during storage. Conclusions: Centrifugation through a low density colloid can eliminate or reduce bacterial contamination in wild boar cumshots without using antibiotics. In addition, it is possible to collect wild boar ejaculations without bacterial contamination by paying strict attention to hygiene.

Gabriel Gonzalez

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