Application of next generation sequencing technology on contamination monitoring in microbiology laboratory

The surveillance and prevention of pathogenic microbiological contamination are the most important tasks of biosecurity management in the laboratory. It is urgent to create an effective and impartial method for evaluating and monitoring such contamination. This study aims to study the usefulness of the next-generation sequencing method (NGS) to detect possible contamination in the microbiology laboratory.

Environmental samples have been taken over several sites in the laboratory, including the internal site of the centrifuge, the bench used for molecular biological tests, the biotechnological cabinet benches used for viral cultivation, pretreatment of clinical samples and nucleic acids, cleaning sites using sterile flocked swabs. The total extracted total nucleic acids were used to build libraries for deep sequencing depending on the protocol of the torrent ion platform. At least 1 g of raw data was obtained for each sample.

Virus and bacteria reads accountedively accounted for 0.01 ± 0.02% and 77.76 ± 12.53% of total reading. Viral sequences should be derived from genes amplification products, contaminated nucleic acids in fetal bovine serum. The readings of environmental microorganisms have also been identified. Our results suggested that the NGS method was able to monitor the contaminant nucleic acids from different sources in the laboratory, demonstrating its promising utility in monitoring and assessing the risk of potential laboratory contamination.

The risk of reagent contamination, remaining DNA and the environment must be taken into account in the data analysis and the interpretation of the results. A simple and fast manual centrifuge has been developed to rotate solutions in the reaction plates of the 96-well polymerase chain (PCR). A commercially available salad spin has been used for this purpose. The acceleration and deceleration of the centrifuge were faster than those of a conventional electric centrifuge using 96 well PCR plates. Solutions in a 96-well PCR plate installed quickly after centrifugation for only 3 s. This light centrifuge can be stored under a laboratory bench or on a shelf and can be placed on the bench only when necessary, while the electric centrifuge is immobile because of its weight and the need for electrical cables. This simple centrifuge is inexpensive, requires minimal effort for manufacturing and can be used anywhere.

Outstanding Anodic Antigen (CAA): Highly sensitive diagnostic biomarker for detecting active schistosome infections – improvement and use during score.

The consortium of schistosomiasis for research and operational assessment was funded in 2008 to conduct research that would support the anti-schistosomiasis programs of countries. The prevalence of schistosomiasis decreases in many places and elimination is increasingly at hand, a sensitive and specific test for detecting Schistosoma Mansoni and Schistosoma Haematobium infection has become an urgent need. After getting a large entrance, the Supported Leiden University Medical Center (CLMC) score to change the serum-based antigen test to use with the urine, simplify the test and improve its sensitivity.

The urine test finally contributed to many of the higher education. For example, in Zanzibar, we have shown that the filtration of the urine, the standard parasite egg detection diagnostic test for S. haematobium, a considerably underestimated prevalence in the low prevalence settings. In Burundi and Rwanda, the outstanding anodic antigen test (CAA) provided critical information on the boundaries of the Kato-Katz parasite eggs detection test for S. Mansoni stool in the settings of the Low prevalence. The other work of CAA supported by the score has shown that frozen and banking urine specimens gave results similar to fresh fees; The pooling of the samples can be a useful and cost-effective approach for monitoring in certain contexts; And the test can be performed in local laboratories with adequate centrifuge capacity.

Application of next generation sequencing technology on contamination monitoring in microbiology laboratory

Laser check of molecular rotation: expanding the utility of an optical centrifuge.

Since its invention in 1999, the optical centrifuge has become a powerful tool for controlling molecular rotation and studying molecular dynamics and molecular properties at extreme levels of rotation excitation. This technique has been applied to a variety of molecular species, from simple linear molecules to symmetrical and asymmetric vertices, molecular ions and chiral enantiomers. Properties of ultra-insulated rotating molecules, so-called molecular superriots have been studied, as well as their collisions with each other and interaction with external fields. The ability of an optical centrifuge to rotate a particular interest molecule depends both on the molecular structure and the parameters of the laser pulse of centrifugal. An interaction between these two factors dictates the usefulness of an optical centrifuge in a specific application. We discuss here the strategy for evaluating and adjusting the properties of the centrifuge to those of molecular rotors and describe two practical examples of optical centrifuges with very different characteristics, implemented experimentally in our laboratory.

We present a versatile tool for the generation of monodisperse droplets in water-fluores-oil in standard reaction tubes in centrifugal stages emulsify. The microfluidic cartridge is designed as an insertion into a standard 2 ml reaction tube and can be treated in standard laboratory centrifuges. It allows a generation of droplets and a subsequent transfer for downstream analysis or subsequent use, requires no specialized device and manufacturing is simple because it consists of two parts only: a structured substrate and a sealing sheet. The structure of the structured substrate is compatible with injection molding to allow large-scale manufacturing. The droplets are generated in fluorinated oil and collected in the reaction tube for later analysis.

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